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Genetic dissection of a pericentromeric region of barley chromosome 6H associated with Fusarium head blight resistance, grain protein content and agronomic traits

Theoretical and Applied Genetics volume 134, pages 3963–3981 (2021)Cite this article

Abstract

Key message

Fine mapping of barley 6H pericentromeric region identified FHB QTL with opposite effects, and high grain protein content was associated with increased FHB severity.

Abstract

Resistance to Fusarium head blight (FHB), kernel discoloration (KD), deoxynivalenol (DON) accumulation and grain protein content (GPC) are important traits for breeding malting barley varieties. Previous work mapped a Chevron-derived FHB QTL to the pericentromeric region of 6H, coinciding with QTL for KD resistance and GPC. The Chevron allele reduced FHB and KD, but unfavorably increased GPC. To determine whether the correlations are caused by linkage or pleiotropy, a fine mapping approach was used to dissect the QTL underlying these quality and disease traits. Two populations, referred to as Gen10 and Gen10/Lacey, derived from a recombinant near-isogenic line (rNIL) were developed. Recombinants were phenotyped for FHB, KD, DON, GPC and other agronomic traits. Three FHB, two DON and two KD QTLs were identified. One of the three FHB QTLs, one DON QTL and one KD QTL were coincident with the GPC QTL, which contains the Hv-NAM1 locus affecting grain protein accumulation. The Chevron allele at the GPC QTL increased GPC and FHB and decreased DON and KD. The other two FHB QTL and the other DON and KD QTL were identified in the regions flanking the Hv-NAM1 locus, and the Chevron alleles decreased FHB, DON and KD. Our results suggested that the QTL associated with FHB, KD, DON and GPC in the pericentromeric region of 6H was controlled by both pleiotropy and tightly linked loci. The rNILs identified in this study with low FHB severity and moderate GPC may be used for breeding malting barley cultivars.

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Availability of data and materials

Phenotype and genotype data of mapping populations are presented in the supplemental materials and deposited in T3 barley (https://triticeaetoolbox.org/barley/) for the Gen10/Lacey population and Data Repository for University of Minnesota (https://doi.org/10.13020/hwkm-ev34) for the Gen10 population. Materials reported in this study are available upon request.

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Acknowledgements

We thank Dr. Shiaoman Chao (USDA-ARS, Fargo, ND) for genotyping the Gen10 population with GBS markers and the Gen10/Lacey population with SSR markers. Edward Schiefelbein and Guillermo Velasquez (Department of Agronomy and Plant Genetics, University of Minnesota) are thanked for assistance with field trials.

Funding

This material is based upon work supported by the U.S. Department of Agriculture, under Agreement Nos. 59-0206-4-021 and 59-0206-8-203. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture.

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Author notes

  1. Lin Li

    Present address: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China

  2. Yadong Huang and Lu Yin have contributed equally.

Authors and Affiliations

  1. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, 55108, USA

    Yadong Huang, Lu Yin, Ahmad H. Sallam, Shane Heinen, Lin Li, Karen Beaubien, Kevin P. Smith & Gary J. Muehlbauer

  2. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA

    Ruth Dill-Macky, Yanhong Dong & Brian J. Steffenson

  3. Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN, 55108, USA

    Gary J. Muehlbauer

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Contributions

GM and KS conceived and directed the study. RD and BS supervised FHB field trials. YD collected and analyzed mycotoxin data. LL and KB aided with genotyping of populations. SH helped with development of the Gen10/Lacey population. YH, LY and AS performed greenhouse and field trials and analyzed genotypic and phenotypic data. YH and LY drafted the manuscript. All authors reviewed and approved the final manuscript.

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Correspondence to Gary J. Muehlbauer.

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Huang, Y., Yin, L., Sallam, A.H. et al. Genetic dissection of a pericentromeric region of barley chromosome 6H associated with Fusarium head blight resistance, grain protein content and agronomic traits. Theor Appl Genet 134, 3963–3981 (2021). https://doi.org/10.1007/s00122-021-03941-9

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