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Identification and molecular mapping of Rps14, a gene conferring broad-spectrum resistance to Phytophthora sojae in soybean

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Abstract

Key message

A soybean landrace carries broad-spectrum resistance to Phytophthora sojae, which is conferred by a single gene, designated Rps14, on the short arm of chromosome 3.

Abstract

Phytophthora sojae is the causative agent for Phytophthora root and stem rot in soybean [Glycine max (L.) Merr.] and can be managed by deployment of resistance to P. sojae (Rps) genes. PI 340,029 is a soybean landrace carrying broad-spectrum resistance to the pathogen. Analysis of an F2 population derived from a cross between PI 340,029 and a susceptible cultivar ‘Williams’ reveals that the resistance to P. sojae race 1 is conferred by a single gene, designated Rps14, which was initially mapped to a 4.5-cM region on the short arm of chromosome 3 by bulked segregant analysis (BSA), and subsequently narrowed to a 1.48 cM region corresponding to 229-kb in the Williams 82 reference genome (Wm82 v2.a1), using F3:4 families derived from the F2 population. Further analysis indicates that the broad-spectrum resistance carried by PI 340,029 is fully attributable to Rps14. The genomic sequences corresponding to the defined Rps14 region from a set of diverse soybean varieties exhibit drastic NBS-LRR gene copy number variation, ranging from 3 to 17 copies. Ultimate isolation of Rps14 would be critical for precise selection and deployment of the gene for soybean protection.

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Acknowledgements

We would like thank Jeff Thompson, Jon Massman and John Woodward for their leadership in the project collaboration that resulted in this manuscript, and Dechun Wang and Wenxiang Wang for providing SNP genotyping service.

Funding

This work was mainly supported by Corteva Agriscience and partially supported by Indiana Soybean Alliance.

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Author notes

  1. Jieqing Ping

    Present address: Seed Technologies and Analytics, BASF Corporation, Morrisville, NC, 27560, USA

  2. Joshua C. Fitzgerald

    Present address: Virginia Agricultural Research and Extension Centers, Virginia Polytechnic Institute and State University, Warsaw, VA, 22572, USA

  3. Liyang Chen and Weidong Wang contribute equally to this work.

Authors and Affiliations

  1. Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA

    Liyang Chen, Weidong Wang, Jieqing Ping, Joshua C. Fitzgerald, Chancelor B. Clark & Jianxin Ma

  2. Crop Production and Pest Control Research Unit, USDA, ARS, West Lafayette, IN, 47907, USA

    Guohong Cai

  3. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA

    Guohong Cai

  4. Research and Development, Corteva AgriscienceTM, Johnston, IA, 50131, USA

    Rajat Aggarwal

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  1. Liyang Chen
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Contributions

JM and RA designed research, LC, WW, JP, JCF, CBC performed research, LC, WW, GC, and JM analyzed data, LC drafted the manuscript, and JM edited the manuscript.

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Correspondence to Rajat Aggarwal or Jianxin Ma.

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Communicated by Dechun Wang.

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Chen, L., Wang, W., Ping, J. et al. Identification and molecular mapping of Rps14, a gene conferring broad-spectrum resistance to Phytophthora sojae in soybean. Theor Appl Genet 134, 3863–3872 (2021). https://doi.org/10.1007/s00122-021-03933-9

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  • DOI: https://doi.org/10.1007/s00122-021-03933-9

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