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Identification and fine mapping of alien fragments associated with enhanced grain weight from Agropyron cristatum chromosome 7P in common wheat backgrounds

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An enhanced grain weight locus from Agropyron cristatum chromosome 7P was verified in two wheat backgrounds, localized to the 7PS1-2 region. Novel translocation lines with this locus were evaluated.

Abstract

Agropyron cristatum is a wild relative of wheat that harbours elite genes for wheat improvement. The wheat-A. cristatum 7P disomic addition line II-5-1 exhibits high grain weight. Here, to dissect the genetic basis of grain weight contributed by A. cristatum chromosome 7P in wheat backgrounds, four segregated populations of the addition line were developed and evaluated in two wheat backgrounds. The results showed that A. cristatum chromosome 7P can stably and significantly increase the grain weight by approximately 2 g, mainly by increasing grain length at different grain weight levels of the wheat background. The locus for increased grain weight from chromosome 7P shows dominant inheritance independent of the wheat background. Moreover, two deletion lines and 23 translocation lines were identified by cytological methods and molecular markers, and an enlarged chromosome 7P bin map was constructed with 158 STS markers and 40 bin intervals. With the genetic populations of these deletion and translocation lines, the genetic locus of increased grain weight was narrowed down to bin 7PS1-2. Two translocation lines (7PT-A18 and 7PT-B4) with smaller 7P chromosomal segments exhibited an increase in grain weight, grain length and grain width simultaneously. These translocation lines carrying the 7PS1-2 chromosomal fragment will be valuable genetic resources for wheat grain weight improvement. Collectively, this study uncovers the grain weight locus from chromosome 7P and provides novel pre-breeding lines with enhanced grain weight.

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Funding

This work was funded by the Chinese Agriculture Research System (CARS-03), the National Natural Science Foundation of China (NSFC) No. 31971879 and the National Key Research and Development Program of China (2016YFD0100102).

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Authors and Affiliations

  1. National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yangyang Sun, Mingjie Lyu, Haiming Han, Shenghui Zhou, Yuqing Lu, Weihua Liu, Xinming Yang, Xiuquan Li, Jinpeng Zhang, Xu Liu & Lihui Li

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  1. Yangyang Sun
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  2. Mingjie Lyu
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  3. Haiming Han
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  4. Shenghui Zhou
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  5. Yuqing Lu
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  6. Weihua Liu
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  7. Xinming Yang
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  8. Xiuquan Li
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  9. Jinpeng Zhang
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  10. Xu Liu
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  11. Lihui Li
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Contributions

LHL and XL conceived the research. YYS and JPZ performed the research and wrote the paper. YYS collected and analysed the data. MJL produced the plant materials, and HMH, SHZ, YQL, WHL, XMY and XQL participated in the preparation of the reagents and materials in this study. All authors contributed to and approved the final manuscript.

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Correspondence to Jinpeng Zhang, Xu Liu or Lihui Li.

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Communicated by Steven S. Xu.

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Sun, Y., Lyu, M., Han, H. et al. Identification and fine mapping of alien fragments associated with enhanced grain weight from Agropyron cristatum chromosome 7P in common wheat backgrounds. Theor Appl Genet 134, 3759–3772 (2021). https://doi.org/10.1007/s00122-021-03927-7

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  • DOI: https://doi.org/10.1007/s00122-021-03927-7