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Dissecting the genetic control of seed coat color in a RIL population of common bean (Phaseolus vulgaris L.)

Abstract

Key message

Three genes associated with the seed coat color in a TU/Musica RIL population were located on a genetic map, and two candidate genes proposed to control black seed coat in the TU genotype were characterized.

Abstract

Seed coat color is an important characteristic of common bean (Phaseolus vulgaris L.) associated with the marketability of dry bean cultivars, quality and nutritional characteristics of seed, as well as response to pathogens. In this study, the genetic control of seed coat color in a recombinant inbred line population (175 lines) obtained from the cross ‘TU’ × ‘Musica’ was investigated. Phenotypic segregation fitted 1:1 for white vs. nonwhite, and 3:1 for brown versus black, indicating the involvement of three independent genes, one controlling white color and two (with epistatic interaction) controlling black color. Using a genetic map built with 842 SNPs, the gene responsible for the white seed coat was mapped on the linkage group Pv07, in the position previously described for the P gene. For the black seed coat phenotype, two genes were mapped to the beginning of chromosomes Pv06 and Pv08, in the positions estimated for the V gene and the complex C locus, respectively, by classical studies. The involvement of these two genomic regions was verified through two crosses between three selected RILs exhibiting complementary and dominant inheritance, in which the TU alleles for both genes resulted in a black phenotype. Two genes involved in the anthocyanin biosynthesis pathway were proposed as candidate genes: Phvul.006G018800 encoding a flavonoid 3′5’hydroxylase and Phvul.008G038400 encoding MYB113 transcription factor. These findings add knowledge to the complex network of genes controlling seed coat color in common bean as well as providing genetic markers to be used in future genetic analysis or plant breeding.

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Acknowledgements

The authors thank M. Bueno, J.A. Poladura, and F. Díaz for their technical assistance.

Funding

This work was supported in part by the Grant AGL2017-87050-R of the AEI-Spanish Government. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 774244.

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Contributions

CGF performed the phenotyping, genomic, and genetic analysis and prepared the manuscript. AC performed the genotyping and genetic analysis. JJF conceived the work and developed the mapping population. All authors have read and approved the last version.

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Correspondence to Carmen García-Fernández.

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The authors declare that they have not conflict of interest.

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The authors declare that the work was original research that has not been published previously, and is not under consideration for publication elsewhere.

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Communicated by Elena Bitocchi.

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García-Fernández, C., Campa, A. & Ferreira, J.J. Dissecting the genetic control of seed coat color in a RIL population of common bean (Phaseolus vulgaris L.). Theor Appl Genet 134, 3687–3698 (2021). https://doi.org/10.1007/s00122-021-03922-y

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  • DOI: https://doi.org/10.1007/s00122-021-03922-y