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Linkage and association mapping and Kompetitive allele-specific PCR marker development for improving grain protein content in wheat

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Linkage and association mapping identified nine candidate intervals for wheat GPC, and large-scale association mapping based on 9 corresponding KASP markers and 1163 F4 breeding lines revealed 3 significant markers.

Abstract

Wheat grain protein content (GPC) is an important quality indicator. The GPC of wheat grown in the middle and lower reaches of the Yangtze River is often low. Marker-assisted selection (MAS) is an effective tool for improving quantitative traits; however, most markers have not been effectively applied in GPC improvement except Gpc-B1, although many loci associated with GPC were identified. In this study, linkage analysis using a recombinant inbred line population from the cross of core parents of Ningmai 9 and Yangmai 158 and association mapping using the local cultivated varieties were performed and nine candidate intervals were identified. The appropriate kompetitive allele-specific PCR (KASP) markers associated with GPC were successfully developed and screened in 1163 F4 breeding lines. Three markers, Kgpc-2B, Kgpc-2D, and Kgpc-4A, were validated to be significantly related to GPC by large-scale association mapping, and they were combined to achieve the highest efficiency to enhance GPC. We applied these markers in 164 F6 breeding lines and obtained 15 lines with high GPC, indicating their high selective efficiency. Further, strategies for gene exploration in the three significant intervals were proposed. These results were expected to provide a novel route for improving GPC in wheat quality breeding.

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (2016YFD0101802 and 2017YFD0100806) and Jiangsu Agricultural Science and Technology Innovation Fund (CX(18)1001).

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Authors and Affiliations

  1. Provincial Key Lab for Agrobiology, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing, 210014, Jiangsu, China

    Peng Jiang, Peng Zhang, Lei Wu, Yi He, Chang Li, Hongxiang Ma & Xu Zhang

  2. Jiangsu Key Lab of Crop Genome & Molecular Breeding/Jiangsu Co-Innovation Center of Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, Jiangsu, China

    Hongxiang Ma

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  1. Peng Jiang
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  2. Peng Zhang
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  3. Lei Wu
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Contributions

PJ, HXM, and XZ conceived and designed the research. PZ, LW, and YH conducted the investigation of phenotype. PJ and CL conducted molecular marker analysis. PJ and PZ analyzed the data and wrote the article. HXM and XZ revised the article. All the authors read and approved the final version for publication.

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Correspondence to Hongxiang Ma or Xu Zhang.

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Communicated by Steven S. Xu.

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Peng Jiang and Peng Zhang these authors should be considered as joint first authors.

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Jiang, P., Zhang, P., Wu, L. et al. Linkage and association mapping and Kompetitive allele-specific PCR marker development for improving grain protein content in wheat. Theor Appl Genet 134, 3563–3575 (2021). https://doi.org/10.1007/s00122-021-03913-z

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