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Identification of three closely linked loci conferring broad-spectrum Phytophthora sojae resistance in soybean variety Tosan-231

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A variable genomic region containing two Harosoy-derived loci related to Rps7 and one Nemashirazu-derived locus confers broad-spectrum Phytophthora sojae resistance in Tosan-231 and is useful for developing resistant cultivars.

Abstract

We investigated resistance to pathotypically variable Phytophthora sojae isolates in the soybean variety Tosan-231, which has broad-spectrum resistance. Mapping analysis using descendent lines from a cross between Shuurei and Tosan-231 demonstrated that a genomic region between SSR markers BARCSOYSSR_03_0209 and BARCSOYSSR_03_0385 (termed “Region T”), confers broad-spectrum resistance in Tosan-231 and contains three closely linked resistance loci. Inoculation tests with 20 P. sojae isolates of different pathotypes and simple sequence repeat (SSR) analysis of progenitors of Tosan-231 facilitated identification and characterization of Rps genes at the three resistance loci. Two resistance genes, RpsT1 and RpsT2, were found to be derived from Harosoy carrying Rps7. This result suggested two mutually exclusive possibilities: (1) either RpsT1 or RpsT2 is Rps7, and the other is a locally functional novel gene; (2) Rps7 is not a single gene but in fact comprises RpsT1 and RpsT2. The resistance locus containing RpsT3 is derived from Nemashirazu, in which Rps genes have remained poorly defined. Moreover, we identified two genomic regions with relatively high recombination frequencies on the basis of mapping information and proposed a strategy to readily assemble useful resistance genes in or around Region T.

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Acknowledgements

This work was supported in part by a Commissioned Project Study entitled “Development of Soybean and Buckwheat Cultivars with Processability and Wide Area Adaptability According to the Users’ Needs” from the Ministry of Agriculture, Forestry and Fisheries of Japan. We thank Dr. M. Hajika for invaluable suggestions and technical staffs of Central Region Agricultural Research Center of NARO for technical assistance.

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Author notes

  1. Naohiro Yamada

    Present address: , Nagano Agricultural Experiment Station, 492 Ogawara, Suzaka, Nagano, 382-0072, Japan

  2. Hajime Akamatsu

    Present address: Business Promotion Office, Department of Regional Strategy, Central Region Agricultural Research Center, National Agriculture and Food Research Organization (NARO), C/O Institute of Vegetable and Floriculture Science, NARO, 360 Kusawa, Ano, Tsu, Mie, 514-2392, Japan

Authors and Affiliations

  1. Hokuriku Research Center, Central Region Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 1-2-1 Inada, Joetsu, Niigata, 943-0193, Japan

    Jun-ichi Matsuoka, Mami Takahashi, Yuhi Kono & Hajime Akamatsu

  2. Institute of Crop Science, NARO, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    Tetsuya Yamada & Koji Takahashi

  3. Nagano Vegetable and Ornamental Crops Experiment Station, 1066-1 Souga, Shiojiri, Nagano, 399-646, Japan

    Naohiro Yamada

  4. Kyushu Okinawa Agricultural Research Center, NARO, 1823-1 Miimachi, Kurume, Fukuoka, 839-8503, Japan

    Jouji Moriwaki

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  1. Jun-ichi Matsuoka
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Contributions

JM and MT designed the study, analyzed the data and wrote the manuscript. JM, MT, and TY performed experiment. YK, NY, KT, JM, MT, and HA revised the manuscript. All authors contributed to and approved the final manuscript.

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Correspondence to Jun-ichi Matsuoka.

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The experiments were performed in accordance with all relevant Japanese laws.

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Communicated by Henry T. Nguyen.

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Matsuoka, Ji., Takahashi, M., Yamada, T. et al. Identification of three closely linked loci conferring broad-spectrum Phytophthora sojae resistance in soybean variety Tosan-231. Theor Appl Genet 134, 2151–2165 (2021). https://doi.org/10.1007/s00122-021-03813-2

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