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Major locus for spontaneous haploid genome doubling detected by a case–control GWAS in exotic maize germplasm

Abstract

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A major locus for spontaneous haploid genome doubling was detected by a case–control GWAS in an exotic maize germplasm. The combination of double haploid breeding method with this locus leads to segregation distortion on genomic regions of chromosome five.

Abstract

Temperate maize (Zea mays L.) breeding programs often rely on limited genetic diversity, which can be expanded by incorporating exotic germplasm. The aims of this study were to perform characterization of inbred lines derived from the tropical BS39 population using different breeding methods, to identify genomic regions showing segregation distortion in lines derived by the DH process using spontaneous haploid genome doubling (SHGD), and use case–control association mapping to identify loci controlling SHGD. Four different sets were used: BS39_DH and BS39_SSD were derived from the BS39 population by DH and single-seed descendent (SSD) methods, and BS39 × A427_DH and BS39 × A427_SSD from the cross between BS39 and A427. A total of 663 inbred lines were genotyped. The analyses of gene diversity and genetic differentiation for the DH sets provided evidence of the presence of a SHGD locus near the centromere of chromosome 5. The case–control GWAS for the DH set also pinpointed this locus. Haplotype sharing analysis showed almost 100% exclusive contribution of the A427 genome in the same region on chromosome 5 of BS39 × A427_DH, presumably due to an allele in this region affecting SHGD. This locus enables DH line production in exotic populations without colchicine or other artificial haploid genome doubling.

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Funding

This work was supported by USDA’s National Institute of Food and Agriculture (grant numbers: IOW04314, IOW01018, IOW05520; IOW05510; NIFA award 2018–51181-28419), the Plant Sciences Institute, Crop Bioengineering Center, R.F. Baker Center for Plant Breeding, K.J. Frey Chair in Agronomy at Iowa State University.

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ALV, UKF and TL design the project and performed the experiments. ALV, IGS, MDK, MH, JC, VCA, LTZ and NB analyzed the data. ALV and IGS wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Iara Gonçalves dos Santos.

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The authors declare that they have no conflict of interest.

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Communicated by Yunbi Xu.

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Verzegnazzi, A.L., dos Santos, I.G., Krause, M.D. et al. Major locus for spontaneous haploid genome doubling detected by a case–control GWAS in exotic maize germplasm. Theor Appl Genet 134, 1423–1434 (2021). https://doi.org/10.1007/s00122-021-03780-8

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  • DOI: https://doi.org/10.1007/s00122-021-03780-8