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A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus

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Abstract

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A mitochondria-localized pentatricopeptide repeat protein was identified by positional cloning and transferred into the hau CMS line, where it successfully restored fertility

Abstract

Cytoplasmic male sterility (CMS) is a maternally inherited trait that can be controlled by restorer-of-fertility (Rf) genes present in the nucleus. The hau CMS was identified as a new form of CMS associated with the mitochondrial transcript orf288; however, a lack of a restorer gene has limited its utilization in Brassica crops. Here, the combination of Brassica 60 K array with bulk segregant analysis and map-based cloning was used to delimit the Rfh locus to an 82.2-kb region on chromosome A09. A candidate gene encoding a mitochondria-localized pentatricopeptide repeat (PPR) protein was identified and transferred into the hau CMS line, where it successfully restored the fertility of the hau CMS plants. Furthermore, the expression analysis showed that Rfh was highly expressed in the flower buds, and the sequence analysis results implied that functional divergence between RFH and rfh could be due to 59 amino acid residue differences in the deduced protein sequences. In addition, a co-separated molecular marker was developed based on the divergent sequences between the dominant and recessive alleles. These results will help enable the heterosis of Brassica crops in the future.

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All data generated or analysed and germplasm involved in this study are included in this published article and its supplementary information files.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31930032 and 31871701) and the National Key Research and Development Programme of China (2016YFD0100804).

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HW, BY, and JS conceived and designed the study. CD, JW, CM, JT, and TF provided advice on the experimental design. HW performed most of the experiments, analysed the data, and wrote the manuscript. QX participated in marker selection and genetic transformation. CW was involved in field hybridisation. HC and XC were involved in vector construction. All authors reviewed and edited this manuscript.

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Correspondence to Jinxiong Shen or Bin Yi.

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We declare that none of the manuscript report data was collected from humans or animals and does not involve any human participants or report any health-related outcomes.

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Communicated by Carlos F. Quiros.

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Wang, H., Xiao, Q., Wei, C. et al. A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus. Theor Appl Genet 134, 1377–1386 (2021). https://doi.org/10.1007/s00122-021-03777-3

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