A major QTL (QLr.cau-2BL) for APR to leaf rust was detected on 2BL; an SSR marker was developed to closely link with QLr.cau-2BL and validated for effectiveness of MAS.
The wheat landrace Hongmazha (HMZ) possesses adult plant resistance (APR) to leaf rust. To detect and validate quantitative trait locus (QTL) for the APR, four wheat populations were assessed for leaf rust severity in a total of eight field and greenhouse experiments. The mapping population Aquileja × HMZ (120 recombinant inbred lines, RILs) was genotyped using 90 K SNP markers. A major QTL (QLr.cau-2BL) was detected between the markers IWB3854 and IWB21922 on chromosome 2BL. IWB3854 and IWB21922 were positioned at approximately 531.14 Mb and 616.48 Mb, respectively, on 2BL of IWGSC RefSeq v1.0 physical map. Based on the sequences between 531.14 and 616.48 Mb on 2BL of IWGSC RefSeq v1.0, 415 simple sequence repeat (SSR) markers were developed. These markers and 28 previously published SSR makers were screened; the resulted polymorphic markers were used to genotype the relatively larger population RL6058 × HMZ (371 RILs). QLr.cau-2BL was mapped within a 1.5 cM interval on 2BL map of RL6058 × HMZ, and a marker (Ta2BL_ssr7) was identified to closely link with QLr.cau-2BL. Effectiveness of selection for QLr.cau-2BL based on Ta2BL_ssr7 was validated using two populations (RL6058 × HMZ F2:3 and Jimai22 × HMZ BC4F2:3). In addition, polymorphism at Ta2BL_ssr7 was detected among a panel of 282 commercial wheat cultivars. We believe, therefore, that Ta2BL_ssr7 should be useful for introducing QLr.cau-2BL into commercial wheat cultivars and for accumulating QLr.cau-2BL with other APR QTL.
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The long arm of chromosome 2B
Analysis of variance
Adult plant resistance
The area under the disease progress curve
- H 2 :
Hongmazha, a wheat landrace
Logarithm of odds
- Lr gene:
Leaf rust resistance gene
Phenotypic variation explained
Quantitative trait locus / loci
Recombinant inbred line
Single nucleotide polymorphism
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We thank Junzhi Wang, Xinfa Mou, Xinhu Wang, Zonghai Bai, and Yanrun Lin for providing excellent technical assistance during the field work. We thank Dr. Ziyi Du for providing the picture of Figure 1. We acknowledge the enormous help of numerous graduate, undergraduate, and high school students who were associated with the Program for Accumulating Quantitative Resistance to Diseases, Plant Pathology Department, China Agricultural University. This study was supported by the China Postdoctoral Science Foundation (2020M670538), National Key Research and Development Program of China (2016YFD02000 and 2018YFD0200500), and Beijing Academy of Agriculture and Forestry Sciences Sci-Tech Innovation Capacity Building Program (KJCX20200115).
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Communicated by Urmil Bansal.
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Zhang, Y., Wang, Z., Quan, W. et al. Mapping of a QTL with major effect on reducing leaf rust severity at the adult plant growth stage on chromosome 2BL in wheat landrace Hongmazha. Theor Appl Genet 134, 1363–1376 (2021). https://doi.org/10.1007/s00122-021-03776-4