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Fine mapping of the qHTB1-1QTL, which confers heat tolerance at the booting stage, using an Oryza rufipogon Griff. introgression line

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The qHTB1-1 QTL, controlling heat tolerance at the booting stage in rice, was fine mapped to a 47.1 kb region containing eight candidate genes. Two positional candidate genes showed significant changes in expression levels under heat stress.

Abstract

High-temperature stress at the booting stage has the potential to significantly limit rice production. An interspecific advanced backcrossed population between the Oryza sativa L. cultivar R53 and the wild Oryza rufipogon Griff accession HHT4 was used as the source material to develop a set of chromosome segment introgression lines to elucidate the genetic mechanism of the qHTB1-1 QTL in heat tolerance. A single-chromosome-segment introgression line, IL01-15, was used to develop secondary populations for the mapping of qHTB1-1 on chromosome 1 for heat tolerance at the booting stage. Using the BC5F2, BC5F3, and BC5F4 populations, we first confirmed qHTB1-1 and validated the phenotypic effect. The qHTB1-1 QTL explained 13.1%, 16.9%, and 17.8% of the phenotypic variance observed in the BC5F2, BC5F3, and BC5F4 generations, respectively. Using homozygous recombinants screened from larger BC6F2 and BC6F3 populations, qHTB1-1 was fine mapped within a 47.1 kb region between markers RM11633 and RM11642. Eight putative predicted genes were annotated in the region, and six genes were predicted to encode expressed proteins. The expression patterns of these six genes demonstrated that LOC_Os01g53160 and LOC_Os01g53220 were highly induced by heat stress in IL01-15 compared to R53. Sequence comparison of the gene-coding regions of LOC_Os01g53160 and LOC_Os01g53220 between R53 and IL01-15 revealed one synonymous and two nonsynonymous SNPs in exons, respectively. Our results provide a basis for identifying the genes underlying qHTB1-1 and indicate that markers linked to the qHTB1-1 locus can be used to improve the heat tolerance of rice at the booting stage by marker-assisted selection.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31560382), the Natural Science Foundation of Jiangxi Province Distinguished Young Scholars, China (Grant No. 2018ACB21026), the Natural Science Foundation of Jiangxi Province (Grant Nos. 20151BAB214013; 2017BAB204020), the Key Research and Development Program of Jiangxi Province, China (Grant No. 20161BBF60129), the Key Laboratory Project of Jiangxi Province, China (Grant No. 20181BCD40010), the Jiangxi Special Fund for Agro-scientific Research in the Collaborative Innovation, China (Grant No. JXXTCXFY201902), and the National Key R&D Program of China, China (Grant No. 2017YFD0301601; Grant No.2018YFD0301103).

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  1. Zhibin Cao and Yao Li contributed equally to this work.

Authors and Affiliations

  1. Rice National Engineering Laboratory (Nanchang), Jiangxi Research and Development Center of Super Rice, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China

    Zhibin Cao, Yao Li, Bohong Zeng, Xiuying Tang, Qizhang Long, Xiaofeng Wu, Yaohui Cai, Linfeng Yuan & Jianlin Wan

  2. Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China

    Yao Li

  3. College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510550, China

    Huiwu Tang

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Contributions

CZB contributed to genotyping, data analysis, and writing; LY contributed to genotyping and data collection; THW contributed to genotyping; TXY contributed to population construction; ZBH contributed to the q-PCR analysis; LQZ provided material and reviewed the manuscript; WXF contributed to population construction; CYH provided material and reviewed the manuscript; WJL and YLF contributed to the experimental design and writing.

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Correspondence to Linfeng Yuan or Jianlin Wan.

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Communicated by Albrecht E. Melchinger.

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Cao, Z., Li, Y., Tang, H. et al. Fine mapping of the qHTB1-1QTL, which confers heat tolerance at the booting stage, using an Oryza rufipogon Griff. introgression line. Theor Appl Genet 133, 1161–1175 (2020). https://doi.org/10.1007/s00122-020-03539-7

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