QTL mapping of resistance to Fusarium oxysporum f. sp. niveum race 2 and Papaya ringspot virus in Citrullus amarus

Branham, Sandra E.; Patrick Wechter, W.; Ling, Kai-Shu; Chanda, Bidisha; Massey, Laura; Zhao, Guangwei; Guner, Nihat; Bello, Marco; Kabelka, Eileen; Fei, Zhangjun; Levi, Amnon

doi:10.1007/s00122-019-03500-3

Original Article
Published: 10 December 2019

QTL mapping of resistance to Fusarium oxysporum f. sp. niveum race 2 and Papaya ringspot virus in Citrullus amarus

Sandra E. Branham ORCID: orcid.org/0000-0002-0373-4566^1,2,
W. Patrick Wechter¹,
Kai-Shu Ling¹,
Bidisha Chanda¹,
Laura Massey¹,
Guangwei Zhao³,
Nihat Guner⁴,
Marco Bello⁴,
Eileen Kabelka⁵,
Zhangjun Fei^6,7 &
Amnon Levi¹

Theoretical and Applied Genetics volume 133, pages 677–687 (2020)Cite this article

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Abstract

Key message

A Citrullus amarus mapping population segregating for resistance to Fusarium oxysporum f. sp. niveum race 2 and Papaya ringspot virus was used to identify novel QTL, important for the improvement in watermelon disease resistance.

Abstract

Multiple disease screens of the USDA Citrullus spp. germplasm collection have highlighted the value of Citrullus amarus (citron melon or wild watermelon) as a resource for enhancing modern watermelon cultivars (Citrullus lanatus) with resistance to a broad range of fungal, bacterial and viral diseases of watermelon. We have generated a genetic population of C. amarus segregating for resistance to two important watermelon diseases: Fusarium wilt (caused by the fungus Fusarium oxysporum f. sp. niveum; Fon race 2) and Papaya ringspot virus-watermelon strain (PRSV-W). QTL mapping of Fon race 2 resistance identified seven significant QTLs, with the major QTL representing a novel genetic source of resistance and an opportunity for gene pyramiding. A single QTL was associated with resistance to PRSV-W, which adhered to expectations of a prior study indicating a single-gene recessive inheritance in watermelon. The resistance loci identified here provide valuable genetic resources for introgression into cultivated watermelon for the improvement in disease resistance.

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Acknowledgements

This research is a part of the Cucurbit Coordinated Agricultural Project (CucCAP) supported by the USDA, National Institute of Food and Agriculture (NIFA), Specialty Crop Research Initiative (SCRI) Grant # 2015-51181-24285, and used resources provided by the SCINet project of the USDA Agricultural Research Service, ARS project number 0500-00093-001-00-D.

Funding

This study was funded, in part, by the US Department of Agriculture (USDA) project number 6080-22000-028-00 and the National Institute of Food and Agriculture, Specialty Crops Research Initiative project number 6080-21000-019-08.

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Affiliations

U.S. Department of Agriculture, Agricultural Research Service, U.S. Vegetable Laboratory, 2700 Savannah Highway, Charleston, SC, 29414, USA
Sandra E. Branham, W. Patrick Wechter, Kai-Shu Ling, Bidisha Chanda, Laura Massey & Amnon Levi
Coastal Research and Education Center, Clemson University, 2700 Savannah Highway, Charleston, SC, 29414, USA
Sandra E. Branham
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Hanghai East Road, Zhengzhou, 450009, China
Guangwei Zhao
Sakata Seed America, 20900 State Road 82, Fort Myers, FL, 33913, USA
Nihat Guner & Marco Bello
Sakata Seed America, 18095 Serene Drive, Morgan Hill, CA, 95037, USA
Eileen Kabelka
Boyce Thompson Institute, Cornell University, 533 Tower Road, Ithaca, NY, 14853, USA
Zhangjun Fei
Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture, Agricultural Research Service, Ithaca, NY, 14853, USA
Zhangjun Fei

Authors

Sandra E. Branham
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W. Patrick Wechter
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Kai-Shu Ling
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Bidisha Chanda
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Laura Massey
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Guangwei Zhao
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Nihat Guner
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Marco Bello
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Eileen Kabelka
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Zhangjun Fei
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Amnon Levi
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Contributions

AL, WPW, SB and KL designed and implemented the experiments. NG, MB and EK developed F₂ and F₃ population and maintained and supplied PRSV inoculum, WPW, SB, KL and AL phenotyped the population. LM and BC assisted in increasing genetic populations, inoculating and maintaining plants in greenhouse, and in conducting DNA isolation, genotyping and ELISA tests. SB analyzed the data. SB, WPW, KL and AL wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Amnon Levi.

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Branham, S.E., Patrick Wechter, W., Ling, KS. et al. QTL mapping of resistance to Fusarium oxysporum f. sp. niveum race 2 and Papaya ringspot virus in Citrullus amarus. Theor Appl Genet 133, 677–687 (2020). https://doi.org/10.1007/s00122-019-03500-3

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Received: 16 May 2019
Accepted: 30 November 2019
Published: 10 December 2019
Issue Date: February 2020
DOI: https://doi.org/10.1007/s00122-019-03500-3

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