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Gb8, a new gene conferring resistance to economically important greenbug biotypes in wheat

Theoretical and Applied Genetics volume 133, pages 615–622 (2020)Cite this article

Abstract

Key message

A new greenbug resistance gene Gb8 conferring broad resistance to US greenbug biotypes was identified in hard red winter wheat line PI 595379-1 and was mapped to the terminal region of chromosome 7DL.

Abstract

Greenbug [Schizaphis graminum (Rondani)] is a worldwide insect pest that poses a serious threat to wheat production. New greenbug resistance genes that can be readily used in wheat breeding are urgently needed. The objective of this study was to characterize a greenbug resistance gene in PI 595379-1, a single plant selection from PI 595379. Genetic analysis of response to greenbug biotype E in an F2:3 population derived from a cross between PI 595379-1 and PI 243735 indicated that a single gene, designated Gb8, conditioned resistance. Linkage analysis placed Gb8 in a 2.7-Mb interval in the terminal bin of chromosome 7DL (7DL3-082-1.0), spanning 595.6 to 598.3 Mb in the Chinese Spring IWGSC RefSeq version 1.0 reference sequence. Gb8 co-segregated with a newly developed SSR marker Xstars508, positioned at 596.4 Mb in the reference sequence. Allelism tests showed that Gb8 was different from three permanently named genes on the same chromosome arm and the estimated genetic distance between Gb8 and Gb3 was 15.35 ± 1.35 cM. Gb8 can be directly used in wheat breeding to enhance greenbug resistance.

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Acknowledgements

We are very grateful to Dr. Robert McIntosh of Sydney University for his insightful comments and suggestions. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.

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Affiliations

  1. Wheat, Peanut, and Other Field Crops Research Unit, USDA-ARS, Stillwater, OK, 74075, USA

    Xiangyang Xu, Genqiao Li & J. Scott Armstrong

  2. Plant and Soil Science Department, Oklahoma State University, Stillwater, OK, 74078, USA

    Brett F. Carver

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  1. Xiangyang Xu
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  2. Genqiao Li
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  3. Brett F. Carver
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  4. J. Scott Armstrong
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Contributions

XX and GL designed and performed the research; BFC and JSA provided overall supports; XX wrote the paper. All authors read, revised and approved the manuscript.

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Correspondence to Xiangyang Xu.

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Communicated by Hermann Buerstmayr.

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Xu, X., Li, G., Carver, B.F. et al. Gb8, a new gene conferring resistance to economically important greenbug biotypes in wheat. Theor Appl Genet 133, 615–622 (2020). https://doi.org/10.1007/s00122-019-03491-1

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  • DOI: https://doi.org/10.1007/s00122-019-03491-1