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Identification of a polymorphism within the Rosa multiflora muRdr1A gene linked to resistance to multiple races of Diplocarpon rosae W. in tetraploid garden roses (Rosa × hybrida)

  • Cindy RouetEmail author
  • Elizabeth A. Lee
  • Travis Banks
  • Joseph O’Neill
  • Rachael LeBlanc
  • Daryl J. Somers
Original Article

Abstract

Key message

A QTL for resistance to several races of black spot co-located with the known Rrd1 locus in Rosa. A polymorphism in muRdr1A linked to black spot resistance was identified and molecular markers were designed.

Abstract

Black spot, caused by Diplocarpon rosae, is one of the most serious foliar diseases of landscape roses that reduces the marketability and weakens the plants against winter survival. Genetic resistance to black spot (BS) exists and race-specific resistance is a good target to implement marker-assisted selection. High-density single nucleotide polymorphism-based genetic maps were created for the female parent of a tetraploid cross between ‘CA60’ and ‘Singing in the Rain’ using genotyping-by-sequencing following a two-way pseudo-testcross strategy. The female linkage map was generated based on 227 individuals and included 31 linkage groups, 1055 markers, with a length of 1980 cM. Race-specific resistance to four D. rosae races (5, 7, 10, 14) was evaluated using a detached leaf assay. BS resistance was also evaluated under natural infection in the field. Resistance to races 5, 10 and 14 of D. rosae and field resistance co-located on chromosome 1. A unique sequence of 32 bp in exon 4 of the muRdr1A gene was identified in ‘CA60’ that co-segregates with D. rosae resistance. Two diagnostic markers, a presence/absence marker and an INDEL marker, specific to this sequence were designed and validated in the mapping population and a backcross population derived from ‘CA60.’ Resistance to D. rosae race 7 mapped to a different location on chromosome 1.

Keywords

Tetraploid Genotyping-by-sequencing (GBS) Single nucleotide polymorphism (SNP) High-density genetic map Marker-assisted breeding Disease resistance 

Notes

Acknowledgments

This work was supported by funding from the Vineland Research and Innovation Centre, Agriculture and Agri-Food Canada Growing Forward 2 project #AIP-P013, the Canadian Nursery Landscape Association, Landscape Manitoba and Landscape Alberta.

Author contributions

CR and DS designed the experiments. CR implemented the experiments, conducted the phenotyping, mapping, and data analysis. TB and JO contributed to GBS library preparation and bioinformatics support. RL contributed to marker development. CR authored the manuscript. DS, TB and EL edited the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2019_3443_MOESM1_ESM.docx (21 kb)
Supplementary file1 (DOCX 21 kb)
122_2019_3443_MOESM2_ESM.docx (15 kb)
Supplementary file2 (DOCX 14 kb)
122_2019_3443_MOESM3_ESM.pdf (39 kb)
Supplementary file3 (PDF 39 kb)
122_2019_3443_MOESM4_ESM.pdf (201 kb)
Supplementary file4 (PDF 201 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Cindy Rouet
    • 1
    • 2
    Email author
  • Elizabeth A. Lee
    • 2
  • Travis Banks
    • 1
  • Joseph O’Neill
    • 1
  • Rachael LeBlanc
    • 1
  • Daryl J. Somers
    • 1
  1. 1.Vineland Research and Innovation CentreVineland StationCanada
  2. 2.Department of Plant AgricultureUniversity of GuelphGuelphCanada

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