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Identification of a new source of stripe rust resistance Yr82 in wheat

Theoretical and Applied Genetics volume 132, pages 3169–3176 (2019)Cite this article

Abstract

Key message

Stripe rust resistance gene, Yr82, was mapped in chromosome 3BL using SNP markers. Yr82 interacted with Yr29 to produce lower stripe rust responses at the adult plant stage.

Abstract

Landrace Aus27969 produced low infection types against Australian Puccinia striiformis f. sp. tritici (Pst) pathotypes. A recombinant inbred line (RIL) F7 population from the Aus27969/Avocet S cross was developed. Monogenic segregation for seedling stripe rust response was observed among the RIL population, and the resistance locus was named Yr82. Bulk segregant analysis performed using the iSelect wheat 90 K Infinium SNP array located Yr82 in the long arm of chromosome 3B. The RIL population was screened against stripe rust under field conditions and was genotyped with targeted genotyping-by-sequencing assay. QTL analysis detected the involvement of chromosomes 1B and 3B in controlling stripe rust resistance carried by Aus27969. Incorporation of Yr82 and marker SNPLr46G22 into the linkage map showed that the QTL in 1B and 3B represented Yr29 and Yr82, respectively. Kompetitive allele-specific PCR (KASP) markers sun KASP_300 and KASP_8775 flanked Yr82 distally and proximally, respectively, each at 2 cM distance. These Yr82-linked markers were polymorphic among 84% of Australian cultivars and can be used for marker-assisted selection of Yr82.

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Acknowledgements

The first author thanks the Australian Government for an International Postgraduate Research Scholarship and Australian Postgraduate Award (IPRS and APA) to pursue Ph.D. studies at the University of Sydney and acknowledges the University of Jaffna for granting study leave. Financial support from the GRDC Australia is gratefully acknowledged.

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  1. Kandiah Pakeerathan

    Present address: Department of Agricultural Biology, The University of Jaffna, Kilinochchi, Sri Lanka

Affiliations

  1. School of Life and Environmental Sciences, Faculty of Science, The University of Sydney Plant Breeding Institute, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia

    Kandiah Pakeerathan, Harbans Bariana, Naeela Qureshi & Urmil Bansal

  2. Centre for AgriBioscience, Agriculture Victoria, AgriBio, 5 Ring Road, Bundoora, VIC, 3083, Australia

    Naeela Qureshi, Debbie Wong & Matthew Hayden

  3. School of Applied Systems Biology, La Trobe University, Bundoora, VIC, 3086, Australia

    Matthew Hayden

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  1. Kandiah Pakeerathan
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Pakeerathan, K., Bariana, H., Qureshi, N. et al. Identification of a new source of stripe rust resistance Yr82 in wheat. Theor Appl Genet 132, 3169–3176 (2019). https://doi.org/10.1007/s00122-019-03416-y

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  • DOI: https://doi.org/10.1007/s00122-019-03416-y

Keywords

  • Adult plant resistance
  • All-stage resistance
  • KASP
  • Puccinia striiformis