Production of a complete set of wheat–barley group-7 chromosome recombinants with increased grain β-glucan content
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Wheat–barley group-7 recombinant chromosomes were selected using molecular cytogenetics and SNP markers; increased grain β-glucan content was observed in wheat plants with two and four copies of HvCslF6.
The soluble dietary fiber (1–3)(1–4) mixed linked β-d-glucan from cereal grains is a valuable component of a healthy diet, which reduces risks of coronary disease and diabetes. Although wheat is an important cereal crop providing a substantial portion of daily calories and protein intake in the human diet, it has a low level of β-glucan. Owing to the plasticity of the polyploid wheat genome, agronomically important traits absent in the wheat primary gene pool can be introgressed from distant relatives. Barley (Hordeum vulgare L.) has a high grain β-glucan content. Earlier, we introgressed this trait into wheat in the form of whole arm compensating Robertsonian translocations (RobT) involving group-7 chromosomes of barley and all three sub-genomes of hexaploid wheat (Triticum aestivum L). In the presented research, we shortened the barley 7HL arms in these RobTs to small pericentromeric segments, using induced wheat–barley homoeologous recombination. The recombinants were selected using SNP markers and molecular cytogenetics. Plants, comprising barley cellulose synthase-like F6 gene (HvCslF6), responsible for β-glucan synthesis, had a higher grain β-glucan content than the wheat control. Three wheat–barley group-7 recombinant chromosomes involving the A, B and D sub-genomes laid the basis for a multiple-copy gene introgression to hexaploid wheat. It is hypothesized that further increases in the β-glucan content in wheat grain can be obtained by increasing the number of HvCslF6 copies through combining several recombinant chromosomes in one line. The wheat lines with four copies of HvCslF6 exceeded the β-glucan content of the lines with two copies.
We thank W. John Raupp for critical editorial review of the manuscript, Duane Wilson for technical assistance, Dr. Gengjun Chen for providing equipment for grain moisture measurement; Dr. Alina Akhunova for real time PCR and spectrophotometry equipment; Drs. Anita Dille and James Stack for providing growth chambers. This is contribution number 19-288-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502, U.S.A.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Alaux M, Rogers J, Letellier T, Flores R, Alfama F, Pommier C, Mohellibi N, Durand S, Kimmel E, Michotey C et al (2018) Linking the International Wheat Genome Sequencing Consortium bread wheat reference genome sequence to wheat genetic and phenomic data. Genome Biol 19:111CrossRefPubMedPubMedCentralGoogle Scholar
- Burton RA, Collins HM, Kibble NAJ, Smith JA, Shirley NJ, Jobling SA, Henderson M, Singh RR, Pettolino F, Wilson SM, Bird AR, Topping DL, Bacic A, Fincher GB (2011) Over-expression of specific HvCslF cellulose synthase-like genes in transgenic barley increases the levels of cell wall (1,3;1,4)-beta-d-glucans and alters their fine structure. Plant Biotechnol J 9:117–135CrossRefPubMedPubMedCentralGoogle Scholar
- Danilova TV, Zhang G, Liu W, Friebe B, Gill BS (2017b) Homoeologous recombination-based transfer and molecular cytogenetic mapping of a wheat streak mosaic virus and Triticum mosaic virus resistance gene Wsm3 from Thinopyrum intermedium to wheat. Theor Appl Genet 130:549–556CrossRefPubMedPubMedCentralGoogle Scholar
- Darrier B, Rimbert H, Balfourier F, Pingault L, Josselin AA, Servin B, Navarro J, Choulet F, Paux E, Sourdille P (2017) High-resolution mapping of crossover events in the hexaploid wheat genome suggests a universal recombination mechanism. Genetics 206:1373–1388CrossRefPubMedPubMedCentralGoogle Scholar
- FAO (2018) FAOSTAT. Food balance sheets. Crop production. http://www.fao.org/faostat/en/#compare (verified 25 June 2018). FAO, Rome, Italy
- Havrlentova M, Kraic J (2006) Content of beta-d-glucan in cereal grains. J Food Nutr Res 45:97–103Google Scholar
- Jordan KW, Wang S, He F, Chao S, Lun Y, Paux E, Sourdille P, Sherman J, Akhunova A, Blake NK, Pumphrey MO, Glover K, Dubcovsky J, Talbert L, Akhunov ED (2018) The genetic architecture of genome-wide recombination rate variation in allopolyploid wheat revealed by nested association mapping. Plant J 95:1039–1054CrossRefPubMedPubMedCentralGoogle Scholar
- Marcotuli I, Gadaleta A, Mangini G, Signorile AM, Zacheo SA, Blanco A, Simeone R, Colasuonno P (2017) Development of a high-density SNP-based linkage map and detection of QTL for beta-glucans, protein content, grain yield per spike and heading time in durum wheat. Int J Mol Sci 18:1329. https://doi.org/10.3390/ijms18061329 CrossRefPubMedCentralGoogle Scholar
- Mayer KFX, Rogers J, Dolezel J, Pozniak C, Eversole K, Feuillet C, Gill B, Friebe B, Lukaszewski AJ, Sourdille P et al (2014) A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome. Science 345:286Google Scholar
- Molnar I, Vrana J, Buresova V, Capal P, Farkas A, Darko E, Cseh A, Kubalakova M, Molnar-Lang M, Dolezel J (2016) Dissecting the U, M, S and C genomes of wild relatives of bread wheat (Aegilops spp.) into chromosomes and exploring their synteny with wheat. Plant J 88:452–467CrossRefPubMedPubMedCentralGoogle Scholar
- Molnár-Láng M, Linc G (2015) Wheat–barley hybrids and introgression lines. In: Molnár-Láng M, Ceoloni C, Doležel J (eds) Alien introgression in wheat. Cytogenetics, molecular biology, and genomics. Springer, Cham, pp 315–346Google Scholar
- Nemeth C, Freeman J, Jones HD, Sparks C, Pellny TK, Wilkinson MD, Dunwell J, Andersson AA, Aman P, Guillon F, Saulnier L, Mitchell RA, Shewry PR (2010) Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-d-glucan in endosperm of wheat. Plant Physiol 152:1209–1218CrossRefPubMedPubMedCentralGoogle Scholar
- Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD, Akhunov ED, Dvorak J, Linkiewicz AM, Ratnasiri A et al (2004) A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics 168:701–712CrossRefPubMedPubMedCentralGoogle Scholar
- Rey E, Abrouk M, Keeble-Gagnere G, Karafiatova M, Vrana J, Balzergue S, Soubigou-Taconnat L, Brunaud V, Martin-Magniette ML, Endo TR, Bartos J, International Wheat Genome Sequencing C, Appels R, Dolezel J (2018) Transcriptome reprogramming due to the introduction of a barley telosome into bread wheat affects more barley genes than wheat. Plant Biotech J 16:1767–1777CrossRefGoogle Scholar
- Saintenac C, Faure S, Remay A, Choulet F, Ravel C, Paux E, Balfourier F, Feuillet C, Sourdille P (2011) Variation in crossover rates across a 3-Mb contig of bread wheat (Triticum aestivum) reveals the presence of a meiotic recombination hotspot. Chromosoma 120:185–198CrossRefPubMedPubMedCentralGoogle Scholar
- Schreiber M, Wright F, MacKenzie K, Hedley PE, Schwerdt JG, Little A et al (2014) The barley genome sequence assembly reveals three additional members of the CslF (1,3;1,4)-β-glucan synthase gene family. PLoS ONE 9(3):e90888. https://doi.org/10.1371/journal.pone.0090888 CrossRefPubMedPubMedCentralGoogle Scholar
- Sears ER (1981) Transfer of alien genetic material to wheat. In: Evans IT, Peacock WJ (eds) Wheat science—today and tomorrow. Cambridge University Press, Cambridge, pp 75–89Google Scholar
- Sibakov J, Lehtinen P, Poutanen K (2013) Cereal brans as dietary fibre ingredients. Elsevier Science, ProQuest Ebook Central, Amsterdam, pp 153–168Google Scholar
- U.S. Department of Health and Human Services and U.S. Department of Agriculture. 2015–2020 dietary guidelines for Americans. 8th edn. December 2015. https://health.gov/dietaryguidelines/2015/guidelines/
- Wicker T, Taudien S, Houben A, Keller B, Graner A, Platzer M, Stein N (2009) A whole-genome snapshot of 454 sequences exposes the composition of the barley genome and provides evidence for parallel evolution of genome size in wheat and barley. Plant J 59:712–722CrossRefPubMedPubMedCentralGoogle Scholar