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Thatcher wheat line RL6149 carries Lr64 and a second leaf rust resistance gene on chromosome 1DS

Theoretical and Applied Genetics volume 132, pages 2809–2814 (2019)Cite this article

Abstract

Key message

The leaf rust resistance gene Lr64 in the Thatcher wheat RL6149 was mapped to chromosome 6AL with SNP and KASP markers and a second leaf rust resistance gene was mapped to chromosome 1DS.

Abstract

RL6149, a near-isogenic line of Thatcher wheat, carries leaf rust resistance gene Lr64 on chromosome arm 6AL. The objective of this study was to develop molecular markers that can be easily used to select wheat lines with Lr64. RL6149 was crossed with Thatcher and F2 plants derived from a single F1 plant were advanced to F6 lines by single seed descent. The 100 F7 recombinant inbred lines (RIL) were inoculated with two races of P.triticina that differed widely for virulence in order to identify resistant and susceptible RIL. Thirty RIL that differed for resistance and the parental lines were genotyped with the 90 K Infinium iSelect single nucleotide polymorphism (SNP) array to find closely linked markers with Lr64. Seven linked SNPs on chromosome arm 6AL were converted into Kompetitive Allele Specific PCR (KASP) markers that were genotyped on the 100 RIL. A genetic linkage map for the seven KASP markers spanned 19.1 cM on chromosome arm 6AL. KASP marker K-IWB59855 was tightly linked to Lr64. A second unexpected gene for leaf rust resistance also segregated in the F7 lines. Four KASP markers that spanned 18.6 cM located the gene on chromosome 1DS. The KASP marker K-IWB38437 was tightly linked to the second leaf rust resistance gene.

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Acknowledgements

We thank K. Xiao for excellent technical assistance.

Author information

Affiliations

  1. United States Department of Agriculture - Agricultural Research Service, Cereal Disease Laboratory, St. Paul, MN, 55108, USA

    J. A. Kolmer

  2. Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA

    A. Bernardo

  3. United States Department of Agriculture - Agricultural Research Service, Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA

    G. Bai

  4. Agriculture Victoria Research, AgriBio, Bundoora, VIC, 3083, Australia

    M. J. Hayden

  5. School of Applied Biology, La Trobe University, Bundoora, VIC, 3083, Australia

    M. J. Hayden

  6. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, 55108, USA

    J. A. Anderson

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  1. J. A. Kolmer
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  2. A. Bernardo
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  3. G. Bai
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  4. M. J. Hayden
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  5. J. A. Anderson
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Contributions

JAK designed the study, developed the mapping population, phenotyped the population, mapped the population, did the quantitative trait analysis, and prepared the manuscript. MJH genotyped the selected RIL, identified SNPs linked to the resistance genes, and revised the manuscript. AB and GB designed the KASP markers and conducted the KASP assays on the population and revised the manuscript. JAA participated in the initial mapping of Lr64 and revised the manuscript.

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Correspondence to J. A. Kolmer.

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Kolmer, J.A., Bernardo, A., Bai, G. et al. Thatcher wheat line RL6149 carries Lr64 and a second leaf rust resistance gene on chromosome 1DS. Theor Appl Genet 132, 2809–2814 (2019). https://doi.org/10.1007/s00122-019-03389-y

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  • DOI: https://doi.org/10.1007/s00122-019-03389-y

Keyword

  • Race-specific resistance
  • Brown rust
  • Seedling resistance gene