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Construction of restorer lines and molecular mapping for restorer gene of hau cytoplasmic male sterility in Brassica napus

Theoretical and Applied Genetics volume 132, pages 2525–2539 (2019)Cite this article

Abstract

Key message

Successfully constructing restorer lines for the hau CMS line and molecular mapping of Rfh to a 94 kb candidate region on chromosome A03 in Brassica napus.

Abstract

Cytoplasmic male sterility is a general phenomenon in almost 200 species, and the interaction between chimeric genes in mitochondria and restorer genes in nucleus may be responsible for restoration of male fertility. Orf288 has been identified as a CMS-associated gene in the hau CMS line of Brassica napus and Brassica juncea; however, the restorer lines/genes have not been found yet. We therefore have successfully constructed two restorer lines in B. napus by extensive testcrossing and have mapped a major restorer gene Rfh to a physical distance of 94 kb on chromosome A03 by whole-genome resequencing and molecular markers. We found that the restorer line is indeed restored to male fertility at histological level. Comparative genomics and collinearity analysis between close relatives revealed that rearrangements and recombination may have happened and thus caused the production of Rfh or components of the restoration of fertility complex. Meanwhile, nuclear backgrounds with multiple loci and temperature were related to the variation and instability of restoration of fertility in three different populations. Our study provides new sights into the coevolution between restorer genes and CMS-associated genes as well as the cultivation of superior hybrids via molecular breeding.

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (Grant Number 2016YFD0101300), the National Natural Science Foundation of China (NSFC Grant Number 31271761), and the Program for Modern Agricultural Industrial Technology System of China (Grant Number CARS-12).

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Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan City, 430070, People’s Republic of China

    Chao Wei, Huadong Wang, Shuangping Heng, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Jinxiong Shen & Tingdong Fu

  2. College of Life Science, Zhaoqing University, Zhaoqing, 526061, People’s Republic of China

    Chao Wei

  3. College of Life Science, Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, People’s Republic of China

    Shuangping Heng

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  1. Chao Wei
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Contributions

CW, SH and JS conceived and designed the study project. JW, CM, JT, and TF gave advises to the experimental design. CW performed most of the experiments and analyses and then wrote the manuscript. HW took part in marker selection, genetic map construction, and field hybridization. All authors reviewed and edited this manuscript.

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Correspondence to Jinxiong Shen.

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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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The project was submitted to NCBI BioProject with BioProject PRJNA472662. The raw reads were deposited in NCBI SRA (Short Read Archive) with the accession number SRP148667. Other data sets supporting the results of this article are included within the article and its additional files.

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122_2019_3368_MOESM1_ESM.tif

Supplementary Figure S1 The distribution and chromosome positions of all 3,770,805 SNPs on the reference genome. (TIFF 2428 kb)

Supplementary Figure S2 ED4 values for selected 78,171 SNPs. (TIFF 3305 kb)

122_2019_3368_MOESM3_ESM.tif

Supplementary Figure S3 Enlarged curves of loess fit of ED4 in the (A) chr.A03, (B) chr.A03_random, and (C) chr.C01_random candidate regions. (TIFF 1832 kb)

122_2019_3368_MOESM4_ESM.xlsx

Supplementary Table S1 The statistics of data produced by sequencing and the evaluation of sequencing quality. (XLSX 9 kb)

Supplementary Table S2 The sequencing depths of coverage of the hauA and hauR samples. (XLSX 9 kb)

Supplementary Table S3 The rates of reads mapped or properly mapped to the reference genome. (XLSX 9 kb)

Supplementary Table S4 The distribution of filtered SNPs on different chromosomes. (XLSX 10 kb)

122_2019_3368_MOESM8_ESM.xlsx

Supplementary Table S5 The corresponding genes and their annotations of Arabidopsis thaliana in the candidate region on chrA03 of B. napus genome. (XLSX 16 kb)

Supplementary Table S6 Primers used to select markers for primary mapping of the restorer gene. (XLSX 29 kb)

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Wei, C., Wang, H., Heng, S. et al. Construction of restorer lines and molecular mapping for restorer gene of hau cytoplasmic male sterility in Brassica napus. Theor Appl Genet 132, 2525–2539 (2019). https://doi.org/10.1007/s00122-019-03368-3

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