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Fine mapping of the wheat powdery mildew resistance gene Pm52 using comparative genomics analysis and the Chinese Spring reference genomic sequence

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Abstract

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A high-resolution genetic linkage map was constructed using the comparative genomics analysis approach and the wheat reference genome, which placed wheat powdery mildew resistance gene Pm52 in a 0.21-cM genetic interval on chromosome arm 2BL.

Abstract

The gene Pm52 confers resistance to powdery mildew and has been previously mapped on chromosome arm 2BL in winter wheat cultivar Liangxing 99. Because of its effectiveness against the disease, this study was initiated to finely map Pm52 using the comparative genomics analysis approach and the wheat reference genomic sequence. Based on the EST sequences that were located in the chromosome region flanking Pm52, four EST-SSR markers were developed, and another nine SSR markers were developed using the comparative genomics technology. These thirteen markers were integrated into a genetic linkage map using an F2:3 subpopulation of the Liangxing 99 × Zhongzuo 9504 cross. Pm52 was mapped within a 3.2-cM genetic interval in the subpopulation that corresponded to a ~40-Mb genomic interval on chromosome arm 2BL of the Chinese Spring reference genome. The Pm52-flanking markers Xicsl163 and Xicsl62 identified 344 recombinant individuals from 8820 F2 plants. Nine SSR markers generated from the Chinese Spring genomic interval were incorporated into a high-resolution genetic linkage map, which placed Pm52 in a 0.21-cM genetic interval corresponding to 5.6-Mb genomic region. The constructed high-resolution genetic linkage map will facilitate the map-based cloning of Pm52 and its marker-assisted selection.

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Acknowledgements

The authors thank Dr. Robert L. Conner, Morden Research and Developmental Center, Agriculture and Agri-Food Canada, for his critical review of the manuscript. The financial support provided by the National Natural Science Foundation of China (31471491, 31871621, and 31501310), the National Key Research and Development Program of China (2017YFD0101000), the Scientific and Technological Research Project of Henan Province of China (172102110110), and the CAAS Innovation Team is gratefully appreciated.

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Authors and Affiliations

  1. National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Peipei Wu, Jinghuang Hu, Jingwei Zou, Dan Qiu, Yahui Li, Teng Li, Hongjun Zhang, Li Yang, Hongwei Liu, Yang Zhou & Hongjie Li

  2. College of Chemistry and Environment Engineering, Pingdingshan University, Pingdingshan, 467000, China

    Jingting Li

  3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Zhiyong Liu

  4. Department of Plant Pathology, China Agricultural University, Beijing, 100193, China

    Peipei Wu & Zhongjun Zhang

  5. College of Life Science and Technology, Harbin Normal University, Harbin, 150080, China

    Yunfeng Qu

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Wu, P., Hu, J., Zou, J. et al. Fine mapping of the wheat powdery mildew resistance gene Pm52 using comparative genomics analysis and the Chinese Spring reference genomic sequence. Theor Appl Genet 132, 1451–1461 (2019). https://doi.org/10.1007/s00122-019-03291-7

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