Theoretical and Applied Genetics

, Volume 132, Issue 4, pp 871–882 | Cite as

Fine mapping and molecular marker development of the Sm gene conferring resistance to gray leaf spot (Stemphylium spp.) in tomato

  • Xiaomei Su
  • Guangtao Zhu
  • Zejun Huang
  • Xiaoxuan Wang
  • Yanmei Guo
  • Baoju Li
  • Yongchen Du
  • Wencai YangEmail author
  • Jianchang GaoEmail author
Original Article


Key message

The tomato gray leaf spot resistance gene Sm was fine-mapped in a 185-kb region through a map-based cloning strategy and genome-wide association study; a candidate gene was proved to be involved in Sm-mediated resistance through transient gene silencing.


Gray leaf spot, caused by Stemphylium spp., is a warm weather foliar disease in tomato (Solanum lycopersicum L). Resistance against gray leaf spot is conferred by a single incompletely dominant gene (Sm) located on chromosome 11. This study aimed to map and identify molecular marker tightly linked to the Sm gene for the use of marker-assisted selection in breeding. Using an F2 population derived from a cross between the resistant line ‘9706’ and the susceptible line ‘Heinz 1706’, the Sm gene was mapped to a 185-kb interval between two markers, InDel343 and InDel-FT-32 on chromosome 11, which was consistent with the result of a genome-wide association study using 289 diverse accessions. An ORF predicted in this region was proved to be involved in Sm-mediated resistance through transient gene silencing and seems to be a good candidate of the Sm locus. To clone the Sm gene, a bacterial artificial chromosome (BAC) library was screened and one BAC clone B80B15 containing the predicted ORF was identified. The analysis of sequence and structure characteristics demonstrated that the candidate gene was not a typical type resistance gene. Additionally, a co-dominant marker Sm-InDel, which produced a 122-bp or 140-bp fragment for resistant or susceptible alleles, respectively, was developed. This marker was validated in 289 germplasm and could be used in marker-assisted selection for gray leaf spot resistance.



This work was supported by the National Key Research and Development Program of China (2016YFD0101703), the National Natural Science Foundation of China (31471874) and the Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture of the People’s Republic of China.

Compliance with ethical standards

Conflict of interest

The authors of this manuscript declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xiaomei Su
    • 1
    • 2
  • Guangtao Zhu
    • 3
  • Zejun Huang
    • 1
  • Xiaoxuan Wang
    • 1
  • Yanmei Guo
    • 1
  • Baoju Li
    • 1
  • Yongchen Du
    • 1
  • Wencai Yang
    • 2
    Email author
  • Jianchang Gao
    • 1
    Email author
  1. 1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Department of Vegetable Science, College of HorticultureChina Agricultural UniversityBeijingChina
  3. 3.The CAAS-YNU Joint Academy of Potato SciencesYunnan Normal UniversityKunmingChina

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