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Meiotic homoeologous recombination-based mapping of wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum

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We physically dissected and mapped wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum based on meiotic homoeologous recombination, providing a unique physical framework for genome studies.

Abstract

Common wheat has a large and complex genome with narrow genetic diversity and various degrees of recombination between the A, B, and D subgenomes. This has limited the homologous recombination-based genome studies in wheat. Here, we exploited meiotic homoeologous recombination for molecular mapping of wheat chromosome 2B and its homoeologue 2S from Aegilops speltoides and 2E from Thinopyrum elongatum. The 2B–2S and 2B–2E recombination was induced by the ph1b mutant, and recovered using molecular markers and fluorescent genomic in situ hybridization (FGISH). A total of 112 2B–2S and 87 2B–2E recombinants involving different chromosome regions were developed and physically delineated by FGISH. The 2B–2S and 2B–2E recombination hotspots mapped to the subterminal regions on both arms. Recombination hotspots with the highest recombination rates mapped to the short arms. Eighty-three 2B–2S and 67 2B–2E recombinants were genotyped using the wheat 90 K SNP arrays. Based on the genotyping results and FGISH patterns of the recombinants, chromosomes 2B, 2S, and 2E were partitioned into 93, 66, and 46 bins, respectively. In total, 1037 SNPs physically mapped onto distinct bins of these three homoeologous chromosomes. A homoeologous recombination-based bin map was constructed for chromosome 2B, providing a unique physical framework for genome studies in wheat and its relatives. Meiotic homoeologous recombination also facilitates gene introgression to diversify the wheat genome for germplasm development. Therefore, homoeologous recombination-based studies enhance understanding of the wheat genome and its homoeologous counterparts from wild grasses, and expand the genetic variability of the wheat genome.

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Acknowledgements

We thank members of the laboratories involved for their help to this research and Drs. Lili Qi and Rebekah Oliver for their critical review of the manuscript. This project is supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-67013-21121 from the USDA National Institute of Food and Agriculture.

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Authors and Affiliations

  1. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    Wei Zhang, Xianwen Zhu, Mingyi Zhang & Xiwen Cai

  2. USDA-ARS, Red River Valley Agricultural Research Center, Fargo, ND, 58102, USA

    Shiaoman Chao & Steven Xu

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  1. Wei Zhang
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  2. Xianwen Zhu
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  3. Mingyi Zhang
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  4. Shiaoman Chao
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  5. Steven Xu
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Correspondence to Xiwen Cai.

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Communicated by P. Heslop-Harrison.

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Zhang, W., Zhu, X., Zhang, M. et al. Meiotic homoeologous recombination-based mapping of wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum. Theor Appl Genet 131, 2381–2395 (2018). https://doi.org/10.1007/s00122-018-3160-0

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