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CsMYB36 is involved in the formation of yellow green peel in cucumber (Cucumis sativus L.)

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A yellow green peel mutant (ygp) in cucumber was caused by a mutation in Csa2G352940 encoding MYB36 transcription factor.

Abstract

Peel color is one of the important agronomic traits of cucumber (Cucumis sativus L.). However, studies on the molecular regulation mechanism of peel color in cucumber are few. In this study, a cucumber yellow green peel mutant (ygp) of cucumber mutagenized with ethylmethylsulfone by using a wild type cucumber with dark green peel was identified. Pigment measurements indicated that the chlorophyll content of the ygp mutant was less than that of the wild type. Genetic analysis revealed that the phenotype of the ygp mutant was monogenic recessive inheritance. MutMap and genotyping results demonstrated that Csa2G352940 (CsMYB36), encoding the transcription factor MYB36, was the causal gene of the ygp mutant in cucumber. CsMYB36 was downregulated in the fruit of the ygp mutant. Transcriptome profile analysis of the fruit peel of the ygp mutant identified 92 candidate genes including genes that encode Casparian strip (CsCASP1) and pigment synthesis protein (CsMYC2) involved in peel color development in cucumber. CsMYB36 may regulate yellow green coloration in cucumber by interacting with these genes. Overall, these results showed that CsMYB36 can regulate the yellow green peel coloration in cucumber.

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Funding

This work was supported by National Natural Science Foundation of China (Grant Number 31701934), University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (Grant Number UNPYSCT-2015001), ‘Academic backbone’ Project of Northeast Agricultural University (Grant Number 16XG05), Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture/Northeast Agricultural University (Grant Number neauhc201601), Natural Science Foundation of Shanghai (Grant Number 15ZR1429800), and The Science and Technology Planning Project of Guangxi Province (Grant Number 1598006-3-2).

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  1. Ning Hao, Yalin Du, and Huiyuan Li have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin, 150030, China

    Ning Hao, Yalin Du, Huiyuan Li, Chao Wang, Chen Wang, Siyu Gong & Tao Wu

  2. College of Horticulture and Landscape Architecture, Northeast Agricultural University, 600 Changjiang Road, Harbin, 150030, China

    Ning Hao, Yalin Du, Huiyuan Li, Chao Wang, Chen Wang, Siyu Gong & Tao Wu

  3. Guangxi Academy of Agricultural Science, 174 Daxuedong Road, Nanning, 530007, China

    Shengmao Zhou

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  1. Ning Hao
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Correspondence to Tao Wu.

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Communicated by Sanwen Huang.

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Hao, N., Du, Y., Li, H. et al. CsMYB36 is involved in the formation of yellow green peel in cucumber (Cucumis sativus L.). Theor Appl Genet 131, 1659–1669 (2018). https://doi.org/10.1007/s00122-018-3105-7

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