GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon
Map-based cloning identified CmGL that encodes a HD-ZIP type IV transcription factor that controls multicellular trichome initiation in melon.
Trichomes are small hairs covering the aerial parts of plants that originate from the epidermal cells, which can protect plants against the damage by insects and pathogens. The regulatory pathway of unicellular trichomes has been well studied in the model plant Arabidopsis. Little is known about the genetic control and regulation of trichome development in melon (Cucumis melo L.) which has multicellular trichomes. In this study, we identified a melon mutant, cmgl, which showed completely glabrous on all aerial organs. A bulked segregant analysis was conducted to identify polymorphic markers for linkage analysis in a population with 256 F2 plants, which allowed to locate the cmgl locus in melon chromosome VIII. Next-generation sequencing-aided marker discovery and fine mapping in a large population with 1536 F2 plants narrowed the candidate gene region to 12 kb that harbored only one candidate gene for cmgl, which encoded a class IV homeodomain-associated leucine zipper transcription factor. Four SNPs in the coding region of the CmGL gene were identified between the two parental lines; a single base substitution from C to A resulted in a premature termination codon and a truncated protein in the cmgl. The SNP was converted into a dCAPS marker, which showed co-segregation in the F2 population and 564 melon accessions. Result of this study will be helpful for better understanding of genetic control of trichome development in melon and marker-assisted selection in developing new cultivars.
This work was supported by grants from the National Natural Science Foundation of China (No. 31601776), the Scientific Innovation Fund of Henan Agricultural University (KJCX2016A15), and the International Scientific and Technological Cooperation Project of Henan Province.
Compliance with ethical standards
The experiments comply with the current laws of the country in which we were performed.
Conflict of interest
The authors declare that they have no conflict of interest.
The genomic resequence datasets of two melon materials M465 and NSL73046 have been deposited into the NCBI sequence read archive (SRA) SRP119828 under accessions SRS2585930 and SRS2585931.
- Chen K, Wallis JW, McLellan MD, Larson DE, Kalicki JM, Pohl CS, McGrath SD, Wendl MC, Zhang Q, Locke DP, Shi X, Fulton RS, Ley TJ, Wilson RK, Ding L, Mardis ER (2009) BreakDancer: an algorithm for high-resolution mapping of genomic structural variation. Nat Methods 6:677–681CrossRefPubMedPubMedCentralGoogle Scholar
- Chen CH, Liu ML, Jiang L, Liu XF, Zhao JY, Yan SS, Yang S, Ren HZ, Liu RY, Zhang XL (2014) Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.). J Exp Bot 65:4943–4958CrossRefPubMedPubMedCentralGoogle Scholar
- Feder A, Burger J, Gao S, Lewinsohn E, Katzir N, Schaffer AA, Meir A, Davidovich-Rikanati R, Portnoy V, Gal-On A, Fei Z, Kashi Y, Tadmor Y (2015) A Kelch domain-containing F-box coding gene negatively regulates flavonoid accumulation in muskmelon. Plant Physiol 169:1714–1726PubMedPubMedCentralGoogle Scholar
- Gregory P, Tingey WM, Ave DA, Bouthyette PY (1986) Potato glandular trichomes: a physicochemical defense mechanism against insects. ACS Symp Ser Am Chem Soc 296:160–167Google Scholar
- Kirkbride JH (1993) Biosystematic monograph of the genus Cucumis (Cucurbitaceae): botanical identification of cucumbers and melons. Parkway Publishers, BooneGoogle Scholar
- Walker AR, Davison PA, Bolognesi-Winfield AC, James CM, Srinivasan N, Blundell TL, EschJJ Marks MD, Gray JC (1999) The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein. Plant Cell 11:1337–1350CrossRefPubMedPubMedCentralGoogle Scholar
- Yang L, Koo DH, Li D, Zhang T, Jiang J, Luan F, Renner SS, Henaff E, Sanseverino W, Garcia-Mas J, Casacuberta J, Senalik DA, Simon PW, Chen J, Weng Y (2014) Next-generation sequencing, FISH mapping and synteny-based modeling reveal mechanisms of decreasing dysploidy in Cucumis. Plant J 77:16–30CrossRefPubMedGoogle Scholar