Theoretical and Applied Genetics

, Volume 131, Issue 3, pp 513–524 | Cite as

Mapping and confirmation of loci for salt tolerance in a novel soybean germplasm, Fiskeby III

  • Tuyen D. Do
  • Tri D. Vuong
  • David Dunn
  • Scotty Smothers
  • Gunvant Patil
  • Dennis C. Yungbluth
  • Pengyin Chen
  • Andrew Scaboo
  • Dong Xu
  • Thomas E. Carter
  • Henry T. Nguyen
  • J. Grover Shannon
Original Article

Abstract

Key message

The confirmation of a major locus associated with salt tolerance and mapping of a new locus, which could be beneficial for improving salt tolerance in soybean.

Abstract

Breeding soybean for tolerance to high salt conditions is important in some regions of the USA and world. Soybean cultivar Fiskeby III (PI 438471) in maturity group 000 has been reported to be highly tolerant to multiple abiotic stress conditions, including salinity. In this study, a mapping population of 132 F2 families derived from a cross of cultivar Williams 82 (PI 518671, moderately salt sensitive) and Fiskeby III (salt tolerant) was analyzed to map salt tolerance genes. The evaluation for salt tolerance was performed by analyzing leaf scorch score (LSS), chlorophyll content ratio (CCR), leaf sodium content (LSC), and leaf chloride content (LCC) after treatment with 120 mM NaCl under greenhouse conditions. Genotypic data for the F2 population were obtained using the SoySNP6K Illumina Infinium BeadChip assay. A major allele from Fiskeby III was significantly associated with LSS, CCR, LSC, and LCC on chromosome (Chr.) 03 with LOD scores of 19.1, 11.0, 7.7 and 25.6, respectively. In addition, a second locus associated with salt tolerance for LSC was detected and mapped on Chr. 13 with an LOD score of 4.6 and an R 2 of 0.115. Three gene-based polymorphic molecular markers (Salt-20, Salt14056 and Salt11655) on Chr.03 showed a strong predictive association with phenotypic salt tolerance in the present mapping population. These molecular markers will be useful for marker-assisted selection to improve salt tolerance in soybean.

Notes

Acknowledgements

This research was supported in part by the Missouri Soybean Merchandising Council and the Missouri Agricultural Experiment Station. Mr. Tuyen Do would like to thank Cuu Long Delta Rice Research Institute and the Vietnam Ministry of Agriculture for a graduate student scholarship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2017_3015_MOESM1_ESM.docx (279 kb)
Supplementary Figure S1: A genetic linkage map was constructed in an F2 population derived from a cross of Williams 82 and Fiskeby III (DOCX 279 kb)
122_2017_3015_MOESM2_ESM.docx (574 kb)
Supplementary Figure S2: Physical positions of the most significant markers associated with salt tolerance, Gm13_38988256 (ss715616164), Gm13_39054715 (ss715616173) and Gm13_3965528 (ss715616176) and three candidate genes (Glyma.13g305700, Glyma.13g305800 and Glyma.13g305900) (http://soybase.org) with salt stress response function in the physical map of Chr. 13 (DOCX 573 kb)
122_2017_3015_MOESM3_ESM.docx (15 kb)
Supplementary material 3 (DOCX 14 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Tuyen D. Do
    • 1
  • Tri D. Vuong
    • 1
  • David Dunn
    • 2
  • Scotty Smothers
    • 2
  • Gunvant Patil
    • 1
  • Dennis C. Yungbluth
    • 1
  • Pengyin Chen
    • 2
  • Andrew Scaboo
    • 1
  • Dong Xu
    • 3
  • Thomas E. Carter
    • 4
  • Henry T. Nguyen
    • 1
  • J. Grover Shannon
    • 2
  1. 1.Division of Plant SciencesUniversity of MissouriColumbiaUSA
  2. 2.Division of Plant SciencesUniversity of Missouri, Delta Research CenterPortagevilleUSA
  3. 3.Department of Electric Engineering and Computer Science, Christopher S. Bond Life Sciences CenterUniversity of MissouriColumbiaUSA
  4. 4.Soybean and Nitrogen Fixation UnitUSDA-ARSRaleighUSA

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