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Theoretical and Applied Genetics

, Volume 131, Issue 1, pp 183–191 | Cite as

Fine mapping of the genic male-sterile ms 1 gene in Capsicum annuum L.

  • Kyumi Jeong
  • Doil Choi
  • Jundae Lee
Original Article

Abstract

Key message

The genomic region cosegregating with the genic male-sterile ms 1 gene of Capsicum annuum L. was delimited to a region of 869.9 kb on chromosome 5 through fine mapping analysis. A strong candidate gene, CA05g06780, a homolog of the Arabidopsis MALE STERILITY 1 gene that controls pollen development, was identified in this region.

Abstract

Genic male sterility caused by the ms 1 gene has been used for the economically efficient production of massive hybrid seeds in paprika (Capsicum annuum L.), a colored bell-type sweet pepper. Previously, a CAPS marker, PmsM1-CAPS, located about 2–3 cM from the ms 1 locus, was reported. In this study, we constructed a fine map near the ms 1 locus using high-resolution melting (HRM) markers in an F2 population consisting of 1118 individual plants, which segregated into 867 male-fertile and 251 male-sterile plants. A total of 12 HRM markers linked to the ms 1 locus were developed from 53 primer sets targeting intraspecific SNPs derived by comparing genome-wide sequences obtained by next-generation resequencing analysis. Using this approach, we narrowed down the region cosegregating with the ms 1 gene to 869.9 kb of sequence. Gene prediction analysis revealed 11 open reading frames in this region. A strong candidate gene, CA05g06780, was identified; this gene is a homolog of the Arabidopsis MALE STERILITY 1 (MS1) gene, which encodes a PHD-type transcription factor that regulates pollen and tapetum development. Sequence comparison analysis suggested that the CA05g06780 gene is the strongest candidate for the ms 1 gene of paprika. To summarize, we developed a cosegregated marker, 32187928-HRM, for marker-assisted selection and identified a strong candidate for the ms 1 gene.

Notes

Acknowledgements

This research was supported by the “Cooperative Research Program for Agriculture Science & Technology Development” (Project no. PJ 01229801), Rural Development Administration, Republic of Korea. We are grateful to Soung-Woo Park for providing paprika F2 segregating seeds (NHSEED, South Korea).

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest to declare.

Supplementary material

122_2017_2995_MOESM1_ESM.docx (1.5 mb)
Supplementary material 1 (DOCX 1540 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of HorticultureChonbuk National UniversityJeonjuSouth Korea
  2. 2.Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life SciencesSeoul National UniversitySeoulSouth Korea

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