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Novel and major QTL for branch angle detected by using DH population from an exotic introgression in rapeseed (Brassica napus L.)

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Abstract

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A high-density SNP map was constructed and several novel QTL for branch angle across six environments in Brassica napus were identified.

Abstract

Branch angle is a major determinant for the ideotype of a plant, while the mechanisms underlying this trait in Brassica napus remain elusive. Herein, we developed one doubled haploid population from a cross involving one Capsella bursa-pastoris derived B. napus intertribal introgression line with the compressed branches and wooden stems, and constructed a high-density SNP map covering the genetic distance of 2242.14 cM, with an average marker interval of 0.73 cM. After phenotypic measurements across six environments, the inclusive composite interval mapping algorithm was conducted to analyze the QTL associated with branch angle. In single-environment analysis, a total of 17 QTL were detected and mainly distributed on chromosomes A01, A03, A09 and C03. Of these, three major QTL, qBA.A03-2, qBA.C03-3 and qBA.C03-4 were steadily expressed, each explaining more than 10% of the phenotypic variation in at least two environments. Compared with other results on rapeseed branch angle, these major QTL were newly detected. In QTL by environment interactions (QEI) mapping, 10 QTL were identified, and the QTL average effect and QEI effect were estimated. Of these, 7 QTL were detected in both single-environment analysis and QEI mapping. Based on the physical positions of SNPs and the functional annotation of the Arabidopsis thaliana genome, 27 genes within the QTL regions were selected as candidate genes, including early auxin-responsive genes, small auxin-up RNA, auxin/indoleacetic acid and gretchenhagen-3. These results may pave the way for deciphering the genetic control of branch angle in B. napus.

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Acknowledgements

We are grateful for Drs. Liangcai Jiang, Cheng Cui from Crop Research Institute, Sichuan Academy of Agricultural Sciences, and Profs. Dianrong Li, Yonghong Li, Hybrid Rapeseed Research Center of Shanxi Province, for arranging the field experiments in Chengdu and Weinan. The study was supported by National Sci-Tech Support Plan (2013BAD01B03), Hubei Provincial Sci-Tech Support Plan (201604051006045), Science and Technology Special Project of Guizhou Academy of Agricultural Sciences (no. [2017] 08).

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Authors and Affiliations

  1. National Key Lab of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Yusen Shen, Yi Yang, Ensheng Xu, Xianhong Ge & Zaiyun Li

  2. Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang, 550008, People’s Republic of China

    Yang Xiang

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  1. Yusen Shen
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  2. Yi Yang
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  4. Xianhong Ge
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  5. Yang Xiang
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  6. Zaiyun Li
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Correspondence to Yang Xiang or Zaiyun Li.

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The authors declare that the experiments complied with current laws of China.

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The authors declare that they have no conflict of interest.

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Communicated by Benjamin Stich.

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Shen, Y., Yang, Y., Xu, E. et al. Novel and major QTL for branch angle detected by using DH population from an exotic introgression in rapeseed (Brassica napus L.). Theor Appl Genet 131, 67–78 (2018). https://doi.org/10.1007/s00122-017-2986-1

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