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Molecular insights into the origin of the brown rust resistance gene Bru1 among Saccharum species

Abstract

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Analysis of 387 sugarcane clones using Bru 1 diagnostic markers revealed two possible sources of Bru 1 in Chinese cultivars: one from Saccharum spontaneum and another from Saccharum robustum of New Guinea.

Abstract

Sugarcane brown rust (SBR) is an important fungal disease in many sugarcane production areas around the world, and can cause considerable yield losses in susceptible sugarcane cultivars. One major SBR resistance gene, named Bru1, initially identified from cultivar R570, was shown to be a major SBR resistance source in most of the sugarcane producing areas of the world. In this study, by using the two Bru1-associated markers, R12H16 and 9O20-F4, we surveyed the presence of Bru1 in a Chinese sugarcane germplasm collection of 387 clones, consisting of 228 hybrid cultivars bred by different Chinese sugarcane breeding establishments, 54 exotic hybrid cultivars introduced from other countries and 105 clones of sugarcane ancestral species. The Bru1-bearing haplotype was detected in 43.4% of Chinese sugarcane cultivars, 20.4% of exotic hybrid cultivars, and only 3.8% of ancestral species. Among the 33 Chinese cultivars for which phenotypes of resistance to SBR were available, Bru1 was present in 69.2% (18/26) of the resistant clones. Analyses of the allelic sequence variations of R12H16 and 9O20-F4 suggested two possible sources of Bru1 in Chinese cultivars: one from S. spontaneum and another from S. robustum of New Guinea. In addition, we developed an improved Bru1 diagnostic marker, 9O20-F4-HaeIII, which can eliminate all the false results of 9O20-F4-RsaI observed among S. spontaneum, as well as a new dominant Bru1 diagnostic marker, R12E03-2, from the BAC ShCIR12E03. Our results provide valuable information for further efforts of breeding SBR-resistant varieties, searching new SBR resistance sources and cloning of Bru1 in sugarcane.

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Acknowledgements

This work was supported by a startup fund for distinguished scholars of Fujian Agriculture and Forestry University, and the Project of Education and Scientific Research of Young Teacher of Fujian (JA13102). The authors would like to thank Xin Lu for providing facilities and help in obtaining the leaf tissues from NNSGR.

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YL conceived and designed the experiments; HW, PC and YY conducted the experiments; HW and YL processed the data; YL wrote the manuscript; AD reviewed the manuscript; all authors read and approved the manuscript.

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Correspondence to Yun-Hai Lu.

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The authors declare that the experiments presented in this publication comply with the current laws of China and France.

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Communicated by Dr. Antonio Augusto Franco Garcia.

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Wang, HB., Chen, PH., Yang, YQ. et al. Molecular insights into the origin of the brown rust resistance gene Bru1 among Saccharum species. Theor Appl Genet 130, 2431–2443 (2017). https://doi.org/10.1007/s00122-017-2968-3

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  • DOI: https://doi.org/10.1007/s00122-017-2968-3