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Fine-mapping and identification of a novel locus Rsc15 underlying soybean resistance to Soybean mosaic virus

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Abstract

Key message

Rsc15, a novel locus underlying soybean resistance to SMV, was fine mapped to a 95-kb region on chromosome 6. The Rsc15- mediated resistance is likely attributed to the gene GmPEX14 , the relative expression of which was highly correlated with the accumulation of H 2 O 2 along with the activities of POD and CAT during the early stages of SMV infection in RN-9.

Abstract

Soybean mosaic virus (SMV) causes severe yield losses and seed quality deterioration in soybean [Glycine max (L.) Merr.] worldwide. A series of single dominant SMV resistance genes have been identified on respective soybean chromosomes 2, 13 and 14, while one novel locus, Rsc15, underlying resistance to the virulent SMV strain SC15 from soybean cultivar RN-9 has been recently mapped to a 14.6-cM region on chromosome 6. However, candidate gene has not yet been identified within this region. In the present study, we aimed to fine map the Rsc15 region and identify candidate gene(s) for this invaluable locus. High-resolution fine-mapping revealed that the Rsc15 gene was located in a 95-kb genomic region which was flanked by the two simple sequence repeat (SSR) markers SSR_06_17 and BARCSOYSSR_06_0835. Allelic sequence comparison and expression profile analysis of candidate genes inferred that the gene Glyma.06g182600 (designated as GmPEX14) was the best candidate gene attributing for the resistance of Rsc15, and that genes encoding receptor-like kinase (RLK) (i.e., Glyma.06g175100 and Glyma.06g184400) and serine/threonine kinase (STK) (i.e., Glyma.06g182900 and Glyma.06g183500) were also potential candidates. High correlations were established between the relative expression level of GmPEX14 and the hydrogen peroxide (H2O2) concentration and activities of catalase (CAT) and peroxidase (POD) during the early stages of SMV-SC15 infection in RN-9. The results of the present study will be useful in marker-assisted breeding for SMV resistance and will lead to further understanding of the molecular mechanisms of host resistance against SMV.

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Acknowledgements

This work was financially supported through grants from the National Natural Science Foundation of China (Grant Nos. 31671718, 31371646, 31571690), the Fundamental Research Funds for the Central Universities (Y0201600115), the National Soybean Industrial Technology System of China (No. CARS-04), Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), the Fund of Transgenic Breeding for Soybean Resistance to Soybean mosaic virus (No. 2008ZX08004-004) and Changjiang Scholars and Innovative Research Team in University (PCSIRT13073).

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  1. National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing, People’s Republic of China

    Ren Rui, Shichao Liu, Adhimoolam Karthikeyan, Tao Wang, Haopeng Niu, Jinlong Yin, Yunhua Yang, Liqun Wang, Qinghua Yang, Haijian Zhi & Kai Li

  2. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, People’s Republic of China

    Ren Rui, Shichao Liu, Adhimoolam Karthikeyan, Tao Wang, Haopeng Niu, Jinlong Yin, Yunhua Yang, Liqun Wang, Qinghua Yang, Haijian Zhi & Kai Li

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Rui, R., Liu, S., Karthikeyan, A. et al. Fine-mapping and identification of a novel locus Rsc15 underlying soybean resistance to Soybean mosaic virus . Theor Appl Genet 130, 2395–2410 (2017). https://doi.org/10.1007/s00122-017-2966-5

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