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Quantitative trait locus mapping under irrigated and drought treatments based on a novel genetic linkage map in mungbean (Vigna radiata L.)

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Abstract

Key message

A novel genetic linkage map was constructed using SSR markers and stable QTLs were identified for six drought tolerance related-traits using single-environment analysis under irrigation and drought treatments.

Abstract

Mungbean (Vigna radiata L.) is one of the most important leguminous food crops. However, mungbean production is seriously constrained by drought. Isolation of drought-responsive genetic elements and marker-assisted selection breeding will benefit from the detection of quantitative trait locus (QTLs) for traits related to drought tolerance. In this study, we developed a full-coverage genetic linkage map based on simple sequence repeat (SSR) markers using a recombinant inbred line (RIL) population derived from an intra-specific cross between two drought-resistant varieties. This novel map was anchored with 313 markers. The total map length was 1010.18 cM across 11 linkage groups, covering the entire genome of mungbean with a saturation of one marker every 3.23 cM. We subsequently detected 58 QTLs for plant height (PH), maximum leaf area (MLA), biomass (BM), relative water content, days to first flowering, and seed yield (Yield) and 5 for the drought tolerance index of 3 traits in irrigated and drought environments at 2 locations. Thirty-eight of these QTLs were consistently detected two or more times at similar linkage positions. Notably, qPH5A and qMLA2A were consistently identified in marker intervals from GMES5773 to MUS128 in LG05 and from Mchr11-34 to the HAAS_VR_1812 region in LG02 in four environments, contributing 6.40–20.06% and 6.97–7.94% of the observed phenotypic variation, respectively. None of these QTLs shared loci with previously identified drought-related loci from mungbean. The results of these analyses might facilitate the isolation of drought-related genes and help to clarify the mechanism of drought tolerance in mungbean.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31671758), the China Agriculture Research System (CARS-08), the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2013BAD01B05-2) and the Agricultural Science and Technology Innovation Program (ASTIP) of CAAS.

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  1. Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture, The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Changyou Liu, Jing Wu, Lanfen Wang & Shumin Wang

  2. Key Laboratory of Crop Genetics and Breeding of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035, China

    Changyou Liu, Baojie Fan, Zhimin Cao, Qiuzhu Su, Zhixiao Zhang, Yan Wang & Jing Tian

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  1. Changyou Liu
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Communicated by Dr. Matthew N. Nelson.

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122_2017_2965_MOESM1_ESM.pdf

Supplemental Table S1 Primer information for the SSR and EST-SSR markers mapped in this study. Supplemental Table S2 Summary of two linkage maps aligned with the physical map of mungbean. Supplemental Fig. S1 Phenotypic response of two mungbean parents under drought stress. (a) Phenotypic response of two mungbean parents under drought stress in pot culture. (b) Phenotypic response of two mungbean parents under drought stress in the field. Supplemental Fig. S2 Rainfall distribution and soil moisture during the crop growing period at the Shijiazhuang location. (a) Rainfall distribution during the crop growing period. (b) Soil moisture during the crop growing period. A soil moisture meter was used to record observations around noon every 5 days at a 20 cm depth. Supplemental Fig. S3 Population distribution of plant height (PH), maximum leaf area (MLA), biomass (BM), relative water content (RWC), days to first flowering (FLD), and seed yield (Yield) within the RIL population. Supplemental Fig. S4 QTLs for plant height (PH), maximum leaf area (MLA), biomass (BM), relative water content (RWC), days to first flowering (FLD), and seed yield (Yield) in the genetic map of the RIL population. Map distances (cM) are indicated on the left of each linkage group, and marker names are on the right. The bars for each QTL represent the range of the QTL above the LOD (PDF 399 kb)

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Liu, C., Wu, J., Wang, L. et al. Quantitative trait locus mapping under irrigated and drought treatments based on a novel genetic linkage map in mungbean (Vigna radiata L.). Theor Appl Genet 130, 2375–2393 (2017). https://doi.org/10.1007/s00122-017-2965-6

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