Theoretical and Applied Genetics

, Volume 130, Issue 11, pp 2375–2393 | Cite as

Quantitative trait locus mapping under irrigated and drought treatments based on a novel genetic linkage map in mungbean (Vigna radiata L.)

  • Changyou Liu
  • Jing Wu
  • Lanfen Wang
  • Baojie Fan
  • Zhimin Cao
  • Qiuzhu Su
  • Zhixiao Zhang
  • Yan Wang
  • Jing Tian
  • Shumin Wang
Original Article

Abstract

Key message

A novel genetic linkage map was constructed using SSR markers and stable QTLs were identified for six drought tolerance related-traits using single-environment analysis under irrigation and drought treatments.

Abstract

Mungbean (Vigna radiata L.) is one of the most important leguminous food crops. However, mungbean production is seriously constrained by drought. Isolation of drought-responsive genetic elements and marker-assisted selection breeding will benefit from the detection of quantitative trait locus (QTLs) for traits related to drought tolerance. In this study, we developed a full-coverage genetic linkage map based on simple sequence repeat (SSR) markers using a recombinant inbred line (RIL) population derived from an intra-specific cross between two drought-resistant varieties. This novel map was anchored with 313 markers. The total map length was 1010.18 cM across 11 linkage groups, covering the entire genome of mungbean with a saturation of one marker every 3.23 cM. We subsequently detected 58 QTLs for plant height (PH), maximum leaf area (MLA), biomass (BM), relative water content, days to first flowering, and seed yield (Yield) and 5 for the drought tolerance index of 3 traits in irrigated and drought environments at 2 locations. Thirty-eight of these QTLs were consistently detected two or more times at similar linkage positions. Notably, qPH5A and qMLA2A were consistently identified in marker intervals from GMES5773 to MUS128 in LG05 and from Mchr11-34 to the HAAS_VR_1812 region in LG02 in four environments, contributing 6.40–20.06% and 6.97–7.94% of the observed phenotypic variation, respectively. None of these QTLs shared loci with previously identified drought-related loci from mungbean. The results of these analyses might facilitate the isolation of drought-related genes and help to clarify the mechanism of drought tolerance in mungbean.

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31671758), the China Agriculture Research System (CARS-08), the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2013BAD01B05-2) and the Agricultural Science and Technology Innovation Program (ASTIP) of CAAS.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

122_2017_2965_MOESM1_ESM.pdf (399 kb)
Supplemental Table S1 Primer information for the SSR and EST-SSR markers mapped in this study. Supplemental Table S2 Summary of two linkage maps aligned with the physical map of mungbean. Supplemental Fig. S1 Phenotypic response of two mungbean parents under drought stress. (a) Phenotypic response of two mungbean parents under drought stress in pot culture. (b) Phenotypic response of two mungbean parents under drought stress in the field. Supplemental Fig. S2 Rainfall distribution and soil moisture during the crop growing period at the Shijiazhuang location. (a) Rainfall distribution during the crop growing period. (b) Soil moisture during the crop growing period. A soil moisture meter was used to record observations around noon every 5 days at a 20 cm depth. Supplemental Fig. S3 Population distribution of plant height (PH), maximum leaf area (MLA), biomass (BM), relative water content (RWC), days to first flowering (FLD), and seed yield (Yield) within the RIL population. Supplemental Fig. S4 QTLs for plant height (PH), maximum leaf area (MLA), biomass (BM), relative water content (RWC), days to first flowering (FLD), and seed yield (Yield) in the genetic map of the RIL population. Map distances (cM) are indicated on the left of each linkage group, and marker names are on the right. The bars for each QTL represent the range of the QTL above the LOD (PDF 399 kb)

References

  1. Afanador LK, Haley SD, Kelly JD, Beebe S (1993) Adoption of a “mini-prep” DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris L.). Annu Rep Bean Improv Coop 36:10–11Google Scholar
  2. Akmm A, Somta P, Srinives P (2014) Identification and confirmation of quantitative trait loci controlling resistance to mungbean yellow mosaic disease in mungbean [Vigna radiata (L.) Wilczek]. Mol Breed 34:1497–1506CrossRefGoogle Scholar
  3. Anyia AO, Herzog H (2004) Water-use efficiency, leaf area and leaf gas exchange of cowpeas under mid-season drought. Eur JAgron 20:327–339CrossRefGoogle Scholar
  4. Asfaw A, Blair MW (2012) Quantitative trait loci for rooting pattern traits of common beans grown under drought stress versus non-stress conditions. Mol Breed 30:681–695CrossRefGoogle Scholar
  5. Babu RC, Nguyen BD, Chamarerk V, Shanmugasundaram P, Chezhian P, Jeyaprakash P, Ganesh SK, Palchamy A, Sadasivam S, Sarkarung S (2003) Genetic analysis of drought resistance in rice by molecular markers: association between secondary traits and field performance. Crop Sci 43:1457–1469CrossRefGoogle Scholar
  6. Blair MW, Galeano CH, Tovar E, Munoz Torres MC, Castrillon AV, Beebe SE, Rao IM (2012) Development of a Mesoamerican intra-genepool genetic map for quantitative trait loci detection in a drought tolerant x susceptible common bean (Phaseolus vulgaris L.) cross. Mol Breed 29:71–88CrossRefPubMedGoogle Scholar
  7. Boutin SR, Young ND, Olson TC, Yu ZH, Vallejos CE, Shoemaker RC (1995) Genome conservation among three legume genera detected with DNA markers. Genome 38:928–937CrossRefPubMedGoogle Scholar
  8. Bruce WB, Edmeades GO, Barker TC (2002) Molecular and physiological approaches to maize improvement for drought tolerance. J Exp Bot 53:13–25CrossRefPubMedGoogle Scholar
  9. Chaitieng B, Kaga A, Han OK, Wang XW, Wongkaew S, Laosuwan P, Tomooka N, Vaughan DA (2002) Mapping a new source of resistance to powdery mildew in mungbean. Plant Breed 121:521–525CrossRefGoogle Scholar
  10. Chankaew S, Somta P, Sorajjapinun W, Srinives P (2011) Quantitative trait loci mapping of Cercospora leaf spot resistance in mungbean, Vigna radiata (L.) Wilczek. Mol Breed 28:255–264CrossRefGoogle Scholar
  11. Chankaew S, Somta P, Isemura T, Tomooka N, Kaga A, Vaughan DA, Srinives P (2013) Quantitative trait locus mapping reveals conservation of major and minor loci for powdery mildew resistance in four sources of resistance in mungbean [Vigna radiata (L.) Wilczek]. Mol Breed 32:121–130CrossRefGoogle Scholar
  12. Chen HM, Ku HM, Schafleitner R, Bains TS, Kuo GC, Liu CA, Nair RM (2013) The major quantitative trait locus for mungbean yellow mosaic Indian virus resistance is tightly linked in repulsion phase to the major bruchid resistance locus in a cross between mungbean [Vigna radiata (L.) Wilczek] and its wild relative Vigna radiata ssp. sublobata. Euphytica 192:205–216CrossRefGoogle Scholar
  13. Chen H, Wang L, Wang S, Liu C, Blair MW, Cheng X (2015) Transcriptome sequencing of mung bean (Vigna radiate L.) genes and the identification of EST-SSR markers. PLoS One 10:e0120273CrossRefPubMedPubMedCentralGoogle Scholar
  14. Chen J, Prakit S, Chen X, Cui X, Yuan X, Peerasak S (2016) Gene mapping of a mutant mungbean (Vigna radiata L.) using new molecular markers suggests a gene encoding a yuc4-like protein regulates the chasmogamous flower trait. Front. Plant Sci 7:830Google Scholar
  15. Chotechung S, Somta P, Chen J, Yimram T, Chen X, Srinives P (2016) A gene encoding a polygalacturonase-inhibiting protein (PGIP) is a candidate gene for bruchid (Coleoptera: bruchidae) resistance in mungbean (Vigna radiata). Theor Appl Genet 129:1673–1683CrossRefPubMedGoogle Scholar
  16. da Maia LC, Palmieri DA, de Souza VQ, Kopp MM, de Carvalho FIF, de Oliveira AC (2008) SSR locator: tool for simple sequence repeat discovery integrated with primer design and PCR simulation. Int J Plant Genom 2008:1–9Google Scholar
  17. Fatokun CA, Menancio-Hautea DI, Danesh D, Young ND (1992) Evidence for orthologous seed weight genes in cowpea and mung bean based on RFLP mapping. Genetics 132:841–846PubMedPubMedCentralGoogle Scholar
  18. Gaitan-Solis E, Duque MC, Edwards KJ, Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization, and cross-species amplification in phaseolus ssp. Crop Sci 42:426–428CrossRefGoogle Scholar
  19. Grisi MC, Blair MW, Gepts P, Brondani C, Pereira PA, Brondani RP (2007) Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93 × Jalo EEP558. Genet Mol Res 6:691–706PubMedGoogle Scholar
  20. Gupta SK, Bansal R, Gopalakrishna T (2013) Development and characterization of genic SSR markers for mungbean (Vigna radiata (L.) Wilczek). Euphytica 195:245–258CrossRefGoogle Scholar
  21. Hisano H, Sato S, Isobe S, Sasamoto S, Wada T, Matsuno A, Fujishiro T, Yamada M, Nakayama S, Nakamura Y, Watanabe S, Harada K, Tabata S (2007) Characterization of the soybean genome using EST-derived microsatellite markers. DNA Res 14:271–281CrossRefPubMedGoogle Scholar
  22. Humphry M, Konduri V, Lambrides C, Magner T (2002) Development of a mungbean (Vigna radiata) RFLP linkage map and its comparison with lablab (Lablab purpureus) reveals a high level of colinearity between the two genomes. Theor Appl Genet 105:160–166CrossRefPubMedGoogle Scholar
  23. Humphry ME, Magner T, Mcintyre CL, Aitken EA, Liu CJ (2003) Identification of a major locus conferring resistance to powdery mildew (Erysiphe polygoni DC) in mungbean (Vigna radiata L. Wilczek) by QTL analysis. Genome 46:738–744CrossRefPubMedGoogle Scholar
  24. Isemura T, Kaga A, Tabata S, Somta P, Srinives P, Shimizu T, Jo U, Vaughan DA, Tomooka N (2012) Construction of a genetic linkage map and genetic analysis of domestication related traits in mungbean (Vigna radiata). PLoS One 7:e41304CrossRefPubMedPubMedCentralGoogle Scholar
  25. Islam MR, Hamid A, Khaliq QA, Ahmed JU, Haque MM, Karim MA (2007) Genetic variability in flooding tolerance of mungbean (Vigna radiata, L. wilczek) genotypes. Euphytica 156:247–255CrossRefGoogle Scholar
  26. Jaganathan D, Thudi M, Kale S, Azam S, Roorkiwal M, Gaur PM, Kishor PB, Nguyen H, Sutton T, Varshney RK (2015) Genotyping-by-sequencing based intra-specific genetic map refines a “QTL-hotspot” region for drought tolerance in chickpea. Mol Genet Genomics 290:559–571CrossRefPubMedGoogle Scholar
  27. Kaga A, Ishimoto M (1998) Genetic localization of a bruchid resistance gene and its relationship to insecticidal cyclopeptide alkaloids, the vignatic acids, in mungbean (Vigna radiata L. Wilczek). Mol Genet Genomics 258:378–384CrossRefGoogle Scholar
  28. Kaga A, Isemura T, Tomooka N, Vaughan DA (2008) The genetics of domestication of the azuki bean (Vigna angularis). Genetics 178:1013–1036CrossRefPubMedPubMedCentralGoogle Scholar
  29. Kajonphol T, Sangsiri C, Somta P, Toojinda T, Srinives P (2012) Ssr map construction and quantitative trait loci (qtl) identification of major agronomic traits in mungbean (Vigna radiata (L.) wilczek). Sabrao J Breed Genet 44:71–86Google Scholar
  30. Kang YJ, Kim SK, Kim MY, Lestari P, Kim KH, Ha BK, Jun TH, Hwang WJ, Lee T, Lee J, Shim S, Yoon MY, Jang YE, Han KS, Taeprayoon P, Yoon N, Somta P, Tanya P, Kim KS, Gwag JG, Moon JK, Lee YH, Park BS, Bombarely A, Doyle JJ, Jackson SA, Schafleitner R, Srinives P, Varshney RK, Lee SH (2014) Genome sequence of mungbean and insights into evolution within Vigna species. Nat Commun 5:5443CrossRefPubMedPubMedCentralGoogle Scholar
  31. Kasettranan W, Somta P, Srinives P (2010) Mapping ff quantitative trait loci controlling powdery mildew resistance in mungbean (Vigna radiata (L.) Wilczek). J Crop Sci Biot 13:155–161CrossRefGoogle Scholar
  32. Kitsanachandee R, Somta P, Chatchawankanphanich O, Akhtar KP, Shah TM, Nair RM, Bains TS, Sirari A, Kaur L, Srinives P (2013) Detection of quantitative trait loci for mungbean yellow mosaic India virus (MYMIV) resistance in mungbean (Vigna radiata (L.) Wilczek) in India and Pakistan. Breed Sci 63:367–373CrossRefPubMedPubMedCentralGoogle Scholar
  33. Kumar SV, Tan SG, Quah SC, Yusoff K (2002a) Isolation and characterization of seven tetranucleotide microsatellite loci in mungbean, Vigna radiata. Mol Ecol Notes 2:293–295CrossRefGoogle Scholar
  34. Kumar SV, Tan SG, Quah SC, Yusoff K (2002b) Isolation of microsatellite markers in mungbean, Vigna radiata. Mol Ecol Notes 2:96–98CrossRefGoogle Scholar
  35. Lambrides CJ, Lawn RJ, Godwin ID, Manners J, Imrie BC (2000) Two genetic linkage maps of mungbean using RFLP and RAPD markers. Aust J Agric Res 51:415–425CrossRefGoogle Scholar
  36. Li C, Fatokun CA, Ubi B, Singh BB, Scoles GJ (2001) Determining genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers. Crop Sci 41:189–197CrossRefGoogle Scholar
  37. Li H, Ribaut JM, Li Z, Wang J (2008) Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations. Theor Appl Genet 116:243–260CrossRefPubMedGoogle Scholar
  38. Li HH, Zhang LY, Wang JK (2010) Analysis and answers to frequently asked questions in quantitative trait locus mapping. Acta Agron Sin 36:918–931CrossRefGoogle Scholar
  39. Liu H (2012) Current situation and development prospect of mungbean production in China. Agric Outlook 8:36–39Google Scholar
  40. Liu C, Fan B, Cao Z, Su Q, Wang YAN, Zhang Z, Wu J, Tian J (2016) A deep sequencing analysis of transcriptomes and the development of EST-SSR markers in mungbean (Vigna radiata). J Genet 95:527–535CrossRefPubMedGoogle Scholar
  41. Lu Y, Xu J, Yuan Z, Hao Z, Xie C, Li X, Shah T, Lan H, Zhang S, Rong T, Xu Y (2012) Comparative LD mapping using single SNPs and haplotypes identifies QTL for plant height and biomass as secondary traits of drought tolerance in maize. Mol Breed 30:407–418CrossRefGoogle Scholar
  42. Manavalan LP, Guttikonda SK, Tran LS, Nguyen HT (2009) Physiological and molecular approaches to improve drought resistance in soybean. Plant Cell Physiol 50:1260–1276CrossRefPubMedGoogle Scholar
  43. McKay JK, Richards JH, Nemali KS, Sen S, Mitchell-Olds T, Boles S, Stahl EA, Wayne T, Juenger TE (2008) Genetics of drought adaptation in Arabidopsis thaliana II. QTL analysis of a new mapping population, Kas-1 × Tsu-1. Evolution 62:3014–3026CrossRefPubMedGoogle Scholar
  44. Mei L, Cheng XZ, Wang SH, Wang LX, Liu CY, Sun L, Xu N, Humphry ME, Lambrides CJ, Li HB, Liu CJ (2009) Relationship between bruchid resistance and seed mass in mungbean based on QTL analysis. Genome 52:589–596CrossRefPubMedGoogle Scholar
  45. Menancio-Hautea D, Fatokun CA, Kumar L, Danesh D, Young ND (1993) Comparative genome analysis of mungbean (Vigna radiata L. Wilczek) and cowpea (V. unguiculata L. Walpers) using RFLP mapping data. Theor Appl Genet 86:797–810CrossRefPubMedGoogle Scholar
  46. Meng L, Li HH, Zhang LY, Wang JK (2015) Qtl icimapping: integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J 3:269–283CrossRefGoogle Scholar
  47. Miyagi M, Humphry M, Ma ZY, Lambrides CJ, Bateson M, Liu CJ (2004) Construction of bacterial artificial chromosome libraries and their application in developing PCR-based markers closely linked to a major locus conditioning bruchid resistance in mungbean (Vigna radiata L. Wilczek). Theor Appl Genet 110:151–156CrossRefPubMedGoogle Scholar
  48. Moe KT, Chung JW, Cho YI, Moon JK, Ku JH, Jung JK, Lee J, Park YJ (2011) Sequence information on simple sequence repeats and single nucleotide polymorphisms through transcriptome analysis of mungbean. J Integr Plant Biol 53:63–73CrossRefPubMedGoogle Scholar
  49. Muchero W, Ehlers JD, Close TJ, Roberts PA (2009) Mapping QTL for drought stress-induced premature senescence and maturity in cowpea [Vigna unguiculata (L.) Walp.]. Theor Appl Genet 118:849–863CrossRefPubMedGoogle Scholar
  50. Pooprompan P, Wasee S, Toojinda T, Abe J, Chanprame S, Srinives P (2006) Molecular marker analysis of days to flowering in vegetable soybean (glycine max (L.) merrill). Kasetsart J Nat Sci 40:2487–2489Google Scholar
  51. Rehman AU, Malhotra RS, Bett K, Tar’an B, Bueckert R, Warkentin TD (2010) Mapping QTL associated with traits affecting grain yield in chickpea (Cicer arietinum L.) under terminal drought stress. Crop Sci 51:141–146Google Scholar
  52. Schafleitner R, Huang SM, Chu SH, Yen JY, Lin CY, Yan MR, Krishnan B, Liu MS, Lo HF, Chen CY, Chen LF, Wu DC, Bui TG, Ramasamy S, Tung CW, Nair R (2016) Identification of single nucleotide polymorphism markers associated with resistance to bruchids (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) and cultivated V. radiata through genotyping by sequencing and quantitative trait locus analysis. BMC Plant Biol 16:159CrossRefPubMedPubMedCentralGoogle Scholar
  53. Seehalak W, Somta P, Sommanas W, Srinives P (2009) Microsatellite markers for mungbean developed from sequence database. Mol Ecol Resour 9:862–864CrossRefPubMedGoogle Scholar
  54. Serraj R, Sinclair TR (2002) Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant Cell Environ 25:333–341CrossRefPubMedGoogle Scholar
  55. Sholihin HDM (2002a) Molecular mapping of drought resistance in mungbean (Vigna radiata): 1. Linkage map in mungbean using AFLP markers. J Bioteknologi Pertanian 7:17–24Google Scholar
  56. Sholihin HDM (2002b) Molecular mapping of drought resistance in mungbean (Vigna radiata): 2. QTL linked to drought resistance. J Bioteknologi Pertanian 7:55–61Google Scholar
  57. Singh D, Singh CK, Taunk J, Tomar RSS (2016) Genetic analysis and molecular mapping of seedling survival drought tolerance gene in lentil (Lens culinaris Medikus). Mol Breed 36:58CrossRefGoogle Scholar
  58. Sompong U, Somta P, Raboy V, Srinives P (2012) Mapping of quantitative trait loci for phytic acid and phosphorus contents in seed and seedling of mungbean (Vigna radiata (L.) Wilczek). Breed Sci 62:87–92CrossRefPubMedPubMedCentralGoogle Scholar
  59. Somta P, Musch W, Kongsamai B, Chanprame S, Nakasathien S, Toojinda T, Sorajjapinun W, Seehalak W, Tragoonrung S, Srinives P (2008) New microsatellite markers isolated from mungbean (Vigna radiata(L.) Wilczek). Mol Ecol Resour 8:1155–1157CrossRefPubMedGoogle Scholar
  60. Somta P, Seehalak W, Srinives P (2009) Development, characterization and cross-species amplification of mungbean (Vigna radiata) genic microsatellite markers. Conserv Genet 10:1939–1943CrossRefGoogle Scholar
  61. Tangphatsornruang S, Somta P, Uthaipaisanwong P, Chanprasert J, Sangsrakru D, Seehalak W, Sommanas W, Tragoonrung S, Srinives P (2009) Characterization of microsatellites and gene contents from genome shotgun sequences of mungbean (Vigna radiata (L.) Wilczek). BMC Plant Biol 9:137CrossRefPubMedPubMedCentralGoogle Scholar
  62. Tomooka N, Vaughan DA, Moss H, Maxted N (2002) The Asian Vigna: genus Vigna subgenus ceratotropis genetic resources. Kluwer, DordrechtCrossRefGoogle Scholar
  63. Tuberosa R, Salvi S (2006) Genomics-based approaches to improve drought tolerance of crops. Trends Plant Sci 11:405–412CrossRefPubMedGoogle Scholar
  64. Varshney RK, Thudi M, Nayak SN, Gaur PM, Kashiwagi J, Krishnamurthy L, Jaganathan D, Koppolu J, Bohra A, Tripathi S, Rathore A, Jukanti AK, Jayalakshmi V, Vemula A, Singh SJ, Yasin M, Sheshshayee MS, Viswanatha KP (2014) Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.). Theor Appl Genet 127:445–462CrossRefPubMedGoogle Scholar
  65. Voorrips RE (2002) MapChart: software for the Graphical Presentation of Linkage Maps and QTLs. J Hered 93:77–78CrossRefPubMedGoogle Scholar
  66. Wang XW, Kaga A, Tomooka N, Vaughan DA (2004) The development of SSR markers by a new method in plants and their application to gene flow studies in azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi]. Theor Appl Genet 109:352–360PubMedGoogle Scholar
  67. Wang LF, Jing WU, Jing RL, Cheng XZ, Wang SM (2014) Drought resistance identification of mungbean germplasm resources at bud stage. J Plant Genet Resour 15:498–503Google Scholar
  68. Wang LF, Jing WU, Jing RL, Cheng XZ, Wang SM (2015a) Drought resistance identification of mungbean germplasm resources at seedlings stage. Acta Agron Sin 41:145–153CrossRefGoogle Scholar
  69. Wang LF, Jing WU, Jing RL, Cheng XZ, Wang SM (2015b) Identification of mungbean germplasm resources resistant to drought at adult stage. Acta Agron Sin 41:1287–1294CrossRefGoogle Scholar
  70. Wang LX, Elbaidouri M, Abernathy B, Chen HL, Wang SH, Lee SH, Jackson SA, Cheng XZ (2015c) Distribution and analysis of SSR in mungbean (Vigna radiata L.) genome based on an SSR-enriched library. Mol Breed 35:1–10CrossRefGoogle Scholar
  71. Wang LX, Kikuchi S, Muto C, Naito K, Isemura T, Ishimoto M, Cheng XZ, Kaga A, Tomooka N (2015d) Reciprocal translocation identified in Vigna angularis dominates the wild population in East Japan. J Plant Res 128:653–663CrossRefPubMedGoogle Scholar
  72. Wang LX, Wu CS, Zhong M, Zhao D, Mei L, Chen HL, Wang SH, Liu CJ, Cheng XZ (2016) Construction of an integrated map and location of a bruchid resistance gene in mungbean. Crop J 4:360–366CrossRefGoogle Scholar
  73. Wu CS, Wang LL, Wang SH, Chen HL, Wu JX, Cheng XZ, Yang XM (2014) Construction of a genetic linkage map in mungbean. Sci Agric Sin 47:2088–2098Google Scholar
  74. Yao Y, Chen F, Wang M, Wang J, Ren G (2008) Antidiabetic activity of mungbean extracts in diabetic KK-Ay mice. J Agric Food Chem 56:8869–8873CrossRefPubMedGoogle Scholar
  75. Young ND, Kumar L, Menancio-Hautea D, Danesh D (1992) RFLP mapping of a major bruchid resistance gene in mungbean (Vigna radiata, L. Wilczek). Theor Appl Genet 84:839–844PubMedGoogle Scholar
  76. Young ND, Danesh D, Menanciohautea D, Kumar L (1993) Mapping oligogenic resistance to powdery mildew in mungbean with RFLPs. Theor Appl Genet 87:243–249CrossRefPubMedGoogle Scholar
  77. Zhao D, Cheng XZ, Wang LX, Wang SH, Ma YL (2010) Integration of mungbean (Vigna radiata) genetic linkage map. Acta Agron Sin 36:932–939Google Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Changyou Liu
    • 1
    • 2
  • Jing Wu
    • 1
  • Lanfen Wang
    • 1
  • Baojie Fan
    • 2
  • Zhimin Cao
    • 2
  • Qiuzhu Su
    • 2
  • Zhixiao Zhang
    • 2
  • Yan Wang
    • 2
  • Jing Tian
    • 2
  • Shumin Wang
    • 1
  1. 1.Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture, The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Key Laboratory of Crop Genetics and Breeding of Hebei Province, Institute of Cereal and Oil CropsHebei Academy of Agricultural and Forestry SciencesShijiazhuangChina

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