Development of 25 near-isogenic lines (NILs) with ten BPH resistance genes in rice (Oryza sativa L.): production, resistance spectrum, and molecular analysis

Abstract

Key message

A first set of 25 NILs carrying ten BPH resistance genes and their pyramids was developed in the background of indica variety IR24 for insect resistance breeding in rice.

Abstract

Brown planthopper (Nilaparvata lugens Stal.) is one of the most destructive insect pests in rice. Development of near-isogenic lines (NILs) is an important strategy for genetic analysis of brown planthopper (BPH) resistance (R) genes and their deployment against diverse BPH populations. A set of 25 NILs with 9 single R genes and 16 multiple R gene combinations consisting of 11 two-gene pyramids and 5 three-gene pyramids in the genetic background of the susceptible indica rice cultivar IR24 was developed through marker-assisted selection. The linked DNA markers for each of the R genes were used for foreground selection and confirming the introgressed regions of the BPH R genes. Modified seed box screening and feeding rate of BPH were used to evaluate the spectrum of resistance. BPH reaction of each of the NILs carrying different single genes was variable at the antibiosis level with the four BPH populations of the Philippines. The NILs with two- to three-pyramided genes showed a stronger level of antibiosis (49.3–99.0%) against BPH populations compared with NILs with a single R gene NILs (42.0–83.5%) and IR24 (10.0%). Background genotyping by high-density SNPs markers revealed that most of the chromosome regions of the NILs (BC3F5) had IR24 genome recovery of 82.0–94.2%. Six major agronomic data of the NILs showed a phenotypically comparable agronomic performance with IR24. These newly developed NILs will be useful as new genetic resources for BPH resistance breeding and are valuable sources of genes in monitoring against the emerging BPH biotypes in different rice-growing countries.

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Acknowledgements

We would like to thank Mr. Rodante Abas for assistance in BPH bioassay experiments in the glasshouse and Mr. Allan Trinidad for providing excellent technical assistance during this study. We also thank all members of Novel Gene Resources team of the PB Division, IRRI, for their support during this study. We are grateful to the Global Rice Science Partnership (GRiSP) program of IRRI for financial support (Grant no.: DRPC 2011-134) to carry out this study. We are thankful to IRRI Communication team for carefully editing the manuscript.

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Correspondence to Kshirod K. Jena.

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This study complied with the ethical standards of the Philippines where this research work was conducted.

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Communicated by Dr. Lizhong Xiong.

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Jena, K.K., Hechanova, S.L., Verdeprado, H. et al. Development of 25 near-isogenic lines (NILs) with ten BPH resistance genes in rice (Oryza sativa L.): production, resistance spectrum, and molecular analysis. Theor Appl Genet 130, 2345–2360 (2017). https://doi.org/10.1007/s00122-017-2963-8

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