Identification and mapping of ts (tender spines), a gene involved in soft spine development in Cucumis sativus
Using map-based cloning of ts gene, we identified a new sort of gene involved in the initiation of multicellular tender spine in cucumber.
The cucumber (Cucumis sativus L.) fruit contains spines on the surface, which is an extremely valuable quality trait affecting the selection of customers. In this study, we elaborated cucumber line NC072 with wild type (WT) hard fruit spines and its spontaneous mutant NC073, possessing tender and soft spines on fruits. The mutant trait was named as tender spines (ts), which is controlled by a single recessive nuclear gene. We identified the gene ts by map-based cloning with an F2 segregating population of 721 individuals generated from NC073 and WT line SA419-2. It was located between two markers Indel6239679 and Indel6349344, 109.7 kb physical distance on chromosome 1 containing fifteen putative genes. With sequencing and quantitative reverse transcription-polymerase chain reaction analysis, the Csa1G056960 gene was considered as the most possible candidate gene of ts. In the mutant, Csa1G056960 has a nucleotide change in the 5′ splicing site of the second intron, which causes different splicing to delete the second exon, resulting in a N-terminal deletion in the predicted amino acid sequence. The gene encodes a C-type lectin receptor-like tyrosine-protein kinase which would play an important role in the formation of cucumber fruit. This is firstly reported of a receptor kinase gene regulating the development of multicellular spines/trichomes in plants. The ts allele could accelerate the molecular breeding of cucumber soft spines.
We thank Dr. Dehua Ma (Tianjin Derit Seeds Company Ltd) for kindly offering us the mutant material. We also thank Dr. Yi-qun Weng (Horticulture Department, University of Wisconsin-Madison, USA) for affording information of cucumber genetic map. This study was supported by The National Natural Science Foundation of China (no. 31672148), the Youth of Natural Science Foundation of Jiangsu Province(no: BK20150232), the Youth Fund of the Natural Science and Agri-X Project of Shanghai Jiao Tong University (Agri-X2015002); Shanghai Graduate Education and Innovation Program (Horticulture).
Compliance with ethical standards
The experiments comply with the current laws of the country in which we were performed.
Conflict of interest
The authors declare that they have no conflict of interest.
- Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci 88:9828–9832CrossRefPubMedPubMedCentralGoogle Scholar
- Suo J, Liang X, Pu L, Zhang Y, Xue Y (2003) Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (Gossypium hirsutum L.). Biochimica et Biophysica Acta (BBA) (Gene Structure and Expression) 1630:25–34CrossRefGoogle Scholar
- Walker AR, Davison PA, Bolognesiwinfield AC, James CM, Srinivasan N, Blundell TL, Esch JJ, Marks MD, Gray JC (1999) The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein. Plant Cell 11:1337–1350CrossRefPubMedPubMedCentralGoogle Scholar