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Mapping stripe rust resistance gene YrZH22 in Chinese wheat cultivar Zhoumai 22 by bulked segregant RNA-Seq (BSR-Seq) and comparative genomics analyses

Theoretical and Applied Genetics volume 130, pages 2191–2201 (2017)Cite this article

Abstract

Key message

A stripe rust resistance gene YrZH22 was mapped by combined BSR-Seq and comparative genomics analyses to a 5.92 centimorgan (cM) genetic interval spanning a 4 Mb physical genomic region on wheat chromosome 4BL1.

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most destructive diseases of wheat and severely threatens wheat production worldwide. The widely grown Chinese wheat cultivar Zhoumai 22 is highly resistant to the current prevailing PST race CYR34 (V26). Genetic analysis of F5:6 and F6:7 recombinant inbred line (RIL) populations indicated that adult-plant stripe rust resistance in Zhoumai 22 is controlled by a single gene, temporarily designated YrZH22. By applying bulked segregant RNA-Seq (BSR-Seq), 7 SNP markers were developed and SNP mapping showed that YrZH22 is located between markers WGGB105 and WGGB112 on chromosome arm 4BL. The corresponding genomic regions of the Chinese Spring 4BL genome assembly and physical map of Aegilops tauschii 4DL were selected for comparative genomics analyses to develop nine new polymorphic markers that were used to construct a high-resolution genetic linkage map of YrZH22. YrZH22 was delimited in a 5.92 cM genetic interval between markers WGGB133 and WGGB146, corresponding to 4.1 Mb genomic interval in Chinese Spring 4BL and a 2.2 Mb orthologous genomic region in Ae. tauschii 4DL. The genetic linkage map of YrZH22 will be valuable for fine mapping and positional cloning of YrZH22, and can be used for marker-assisted selection in wheat breeding.

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (2016YFD0101802).

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Author notes

  1. Yong Wang and Jingzhong Xie contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China

    Yong Wang, Huaizhi Zhang, Bingmin Guo, Miaomiao Li, Deyun Zhang, Guanghao Guo & Yan Zhang

  2. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Jingzhong Xie, Yongxing Chen, Ping Lu, Qiuhong Wu & Zhiyong Liu

  3. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Shunzong Ning & Dengcai Liu

  4. Zhoukou Academy of Agriculture Sciences, Zhoukou, 466001, Henan, China

    Shaokui Zou, Jianwei Tang & Guihong Yin

  5. Wheat Institute, Henan Academy of Agriculture Sciences, Zhengzhou, 450002, Henan, China

    Hong Zhao, Xicheng Wang & Tingjie Cao

  6. Crop Research Institute, Sichuan Academy of Agriculture Sciences, Chengdu, 610066, China

    Jun Li & Wuyun Yang

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  1. Yong Wang
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Correspondence to Tingjie Cao, Guihong Yin or Zhiyong Liu.

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Communicated by Beat Keller.

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Wang, Y., Xie, J., Zhang, H. et al. Mapping stripe rust resistance gene YrZH22 in Chinese wheat cultivar Zhoumai 22 by bulked segregant RNA-Seq (BSR-Seq) and comparative genomics analyses. Theor Appl Genet 130, 2191–2201 (2017). https://doi.org/10.1007/s00122-017-2950-0

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  • DOI: https://doi.org/10.1007/s00122-017-2950-0