Abstract
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Six quantitative trait loci (QTL) for Gibberella ear rot resistance in maize were tested in two different genetic backgrounds; three QTL displayed an effect in few near isogenic line pairs.
Abstract
Few quantitative trait loci (QTL) mapping studies for Gibberella ear rot (GER) have been conducted, but no QTL have been verified so far. QTL validation is prudent before their implementation into marker-assisted selection (MAS) programs. Our objectives were to (1) validate six QTL for GER resistance, (2) evaluate the QTL across two genetic backgrounds, (3) investigate the genetic background outside the targeted introgressions. Pairs of near isogenic lines (NILs) segregating for a single QTL (Qger1, Qger2, Qger10, Qger13, Qger16, or Qger21) were developed by recurrent backcross until generation BC3S2. Donor parents (DP) carrying QTL were backcrossed to a susceptible (UH009) and a moderately resistant (UH007) recurrent parent. MAS was performed using five SNP markers covering a region of 40 cM around each QTL. All NILs were genotyped with the MaizeSNP50 assay and phenotyped for GER severity and deoxynivalenol and zearalenone content. Traits were significantly (P < 0.001) intercorrelated. Out of 34 NIL pairs with the UH009 genetic background, three pairs showed significant differences in at least one trait for three QTL (Qger1, Qger2, Qger13). Out of 25 NIL pairs with the UH007 genetic background, five pairs showed significant differences in at least one trait for two QTL (Qger2, Qger21). However, Qger16, Qger10 and Qger13 were most likely false positives. The genetic background possibly affected NIL pairs comparisons due to linkage drag and/or epistasis with residual loci from the DP in non-target regions. In conclusion, validation rates were disappointingly low, which further indicates that GER resistance is controlled by many low-effect QTL.
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Acknowledgments
This research was funded by the Deutsche Forschungsgemeinschaft (DFG), Bonn (Grant ME2260/6-1). The DH lines were produced by KWS SAAT SE (Einbeck, Germany) which also genotyped the NILs and provided the two field locations Gondelsheim and Chartres. We highly acknowledge the help of J. Möhring with the statistical modeling. We are indebted to Sen Han, Jochen Jesse and Birgit Schilling at University of Hohenheim, Dorothee Seyfang (KWS SAAT SE, Gondelsheim), Tierry Crapeau and Christelle Jacquemard (KWS France S.A.R.L., Champhol) for conducting the field trials for this study. We also thank Willem Molenaar for critical reading and suggestions to improve the content and English language of the manuscript.
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Brauner, P.C., Melchinger, A.E., Schrag, T.A. et al. Low validation rate of quantitative trait loci for Gibberella ear rot resistance in European maize. Theor Appl Genet 130, 175–186 (2017). https://doi.org/10.1007/s00122-016-2802-3
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DOI: https://doi.org/10.1007/s00122-016-2802-3