Abstract
Key message
Using map-based cloning, we delimited the Ms - cd1 gene responsible for the male sterile phenotype in B. oleracea to an approximately 39-kb fragment. Expression analysis suggests that a new predicted gene, a homolog of the Arabidopsis SIED1 gene, is a potential candidate gene.
Abstract
A dominant genic male sterile (DGMS) mutant 79-399-3 in Brassica oleracea (B. oleracea) is controlled by a single gene named Ms-cd1, which was genetically mapped on chromosome C09. The derived DGMS lines of 79-399-3 have been successfully applied in hybrid cabbage breeding and commercial hybrid seed production of several B. oleracea cultivars in China. However, the Ms-cd1 gene responsible for the DGMS has not been identified, and the molecular basis of the DGMS is unclear, which then limits its widespread application in hybrid cabbage seed production. In the present study, a large BC9 population with 12,269 individuals was developed for map-based cloning of the Ms-cd1 gene, and Ms-cd1 was mapped to a 39.4-kb DNA fragment between two InDel markers, InDel14 and InDel24. Four genes were identified in this region, including two annotated genes based on the available B. oleracea annotation database and two new predicted open reading frames (ORFs). Finally, a newly predicted ORF designated Bol357N3 was identified as the candidate of the Ms-cd1 gene. These results will be useful to reveal the molecular mechanism of the DGMS and develop more practical DGMS lines with stable male sterility for hybrid seed production in cabbage.
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Acknowledgments
This work was supported by funding from the National Program Fund for Key Basic Research Projects (the 973 Program: 2013CB127000), the National Natural Science Foundation of China (NSFC: 31301784), and the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences. This research work was conducted at the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China.
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Communicated by M. J. Havey.
J. Liang and Y. Ma have equally contributed to the study.
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122_2016_2792_MOESM1_ESM.pdf
Supplementary Fig. S1 The PCR products amplified by 9 two-marker combinations in parental lines and some BC9 individuals. P1: M20 + InDel28, P2: M20 + InDelN31, P3: M20L + InDel28, P4: M20L + InDelN31, P5: InDelN1 + InDelN31, P6: InDelN3 + InDelN31, P7: IndelN4 + IndelN31, P8: IndelN6 + IndelN31, P9: sf32-SSR6 + IndelN31. M: Marker, ck: recurrent parent, S: BC9 sterile individuals, F: BC9 fertile individuals (PDF 64 kb)
122_2016_2792_MOESM2_ESM.pdf
Supplementary Fig. S2 Five putative ORFs of the 39.4 kb (B. oleracea) region identified by Softberry website (PDF 76 kb)
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Liang, J., Ma, Y., Wu, J. et al. Map-based cloning of the dominant genic male sterile Ms-cd1 gene in cabbage (Brassica oleracea). Theor Appl Genet 130, 71–79 (2017). https://doi.org/10.1007/s00122-016-2792-1
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DOI: https://doi.org/10.1007/s00122-016-2792-1