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Theoretical and Applied Genetics

, Volume 129, Issue 8, pp 1519–1529 | Cite as

Molecular and genetic characterization of barley mutants and genetic mapping of mutant rpr2 required for Rpg1-mediated resistance against stem rust

  • Upinder GillEmail author
  • Robert Brueggeman
  • Jayaveeramuthu Nirmala
  • Yuan Chai
  • Brian Steffenson
  • Andris KleinhofsEmail author
Original Article

Abstract

Key message

This study describes the generation, screening, genetic and molecular characterization, and high-resolution mapping of barley mutants susceptible to stem rust ( Puccinia graminis f. sp. tritici ) races MCCF and HKHJ.

Abstract

A single gene, Rpg1, has protected barley cultivars against many races of stem rust pathogen (Puccinia graminis f. sp. tritici) for the last 70 years in the United States and Canada. To identify signaling components of protein product RPG1, we employed a mutagenesis approach. Using this approach, six mutants exhibiting susceptibility to Puccinia graminis f. sp. tritici races MCCF and HKHJ were identified in the gamma irradiated M2 population of resistant cultivar Morex, which carries Rpg1 on chromosome 7H. The mutants retained a functional Rpg1 gene and an apparently functional protein, suggesting that the mutated genes were required for downstream or upstream signaling. Selected mutants were non-allelic, hence each mutant represents a unique gene. Low and high-resolution genetic mapping of the rpr2 mutant identified chromosome 6H (bin 6) as the location of the mutated gene. The target region was reduced to 0.6 cM and gene content analyzed. Based on the published barley genomic sequence, the target region contains approximately 157 genes, including a set that encodes putative leucine-rich receptor-like protein kinases, which may be strong candidates for the gene of interest. Overall, this study presents a strong platform for future map-based cloning of genes identified in this mutant screen.

Keywords

Stem Rust Stem Rust Resistance Allelism Test Race TTKSK Stem Rust Race 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by the National Research Initiative of the United States Department of Agriculture, Cooperative State Research, Education and Extension Service Grant No. 2007-35301-18205 to AK and BJS.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2016_2721_MOESM1_ESM.pdf (333 kb)
Supplementary material 1 (PDF 332 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Upinder Gill
    • 1
    • 5
    Email author
  • Robert Brueggeman
    • 2
  • Jayaveeramuthu Nirmala
    • 1
    • 6
  • Yuan Chai
    • 3
  • Brian Steffenson
    • 3
  • Andris Kleinhofs
    • 1
    • 4
    Email author
  1. 1.Department of Crop and Soil SciencesWashington State UniversityPullmanUSA
  2. 2.Department of Plant PathologyNorth Dakota State UniversityFargoUSA
  3. 3.Department of Plant PathologyUniversity of MinnesotaSt. PaulUSA
  4. 4.School of Molecular BiosciencesWashington State UniversityPullmanUSA
  5. 5.Plant Biology DivisionThe Samuel Roberts Noble FoundationArdmoreUSA
  6. 6.Cereal Disease LaboratoryUSDA-ARSSaint PaulUSA

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