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A novel er1 allele and the development and validation of its functional marker for breeding pea (Pisum sativum L.) resistance to powdery mildew

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A novel er1 allele, er1 -7, conferring pea powdery mildew resistance was characterized by a 10-bp deletion in PsMLO1 cDNA, and its functional marker was developed and validated in pea germplasms.

Abstract

Pea powdery mildew caused by Erysiphe pisi DC is a major disease worldwide. Pea cultivar ‘DDR-11’ is an elite germplasm resistant to E. pisi. To identify the gene conferring resistance in DDR-11, the susceptible Bawan 6 and resistant DDR-11 cultivars were crossed to produce F1, F2, and F2:3 populations. The phenotypic segregation patterns in the F2 and F2:3 populations fit the 3:1 (susceptible:resistant) and 1:2:1 (susceptible homozygotes:heterozygotes:resistant homozygotes) ratios, respectively, indicating that resistance was controlled by a single recessive gene. Analysis of er1-linked markers in the F2 population suggested that the recessive resistance gene in DDR-11 was an er1 allele, which was mapped between markers ScOPE16-1600 and c5DNAmet. To further characterize er1 allele, the cDNA sequences of PsMLO1 from the parents were obtained and a novel er1 allele in DDR-11 was identified and designated as er1-7, which has a 10-bp deletion in position 111–120. The er1-7 allele caused a frame-shift mutation, resulting in a premature termination of translation of PsMLO1 protein. A co-dominant functional marker specific for er1-7 was developed, InDel111–120, which co-segregated with E. pisi resistance in the mapping population. The marker was able to distinguish between pea germplasms with and without the er1-7. Of 161 pea germplasms tested by InDel111–120, seven were detected containing resistance allele er1-7, which was verified by sequencing their PsMLO1 cDNA. Here, a novel er1 allele was characterized and its an ideal functional marker was validated, providing valuable genetic information and a powerful tool for breeding pea resistance to powdery mildew.

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Acknowledgments

We sincerely thank Prof. Dongxu Xu (Zhangjiakou Academy of Agricultural Sciences) for providing the pea materials used in this study. This study was supported by the Modern Agro-industry Technology Research System (CARS-09) and the Crop Germplasm Conservation and Utilization Program (2014NWB030-14) from the Ministry of Agriculture of China and the Scientific Innovation Program of the Chinese Academy of Agricultural Sciences, and the Public Welfare Special Fund (1610092015002-01) from the Institute of Crop Science, Chinese Academy of Agricultural Sciences.

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  1. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China

    Suli Sun, Dong Deng, Zhongyi Wang, Canxing Duan, Xiaofei Wu, Xiaoming Wang, Xuxiao Zong & Zhendong Zhu

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  1. Suli Sun
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  2. Dong Deng
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  3. Zhongyi Wang
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Correspondence to Zhendong Zhu.

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The experiments were performed in accordance with all relevant Chinese laws.

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Communicated by D. A. Lightfoot.

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Sun, S., Deng, D., Wang, Z. et al. A novel er1 allele and the development and validation of its functional marker for breeding pea (Pisum sativum L.) resistance to powdery mildew. Theor Appl Genet 129, 909–919 (2016). https://doi.org/10.1007/s00122-016-2671-9

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