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Identification of molecular variants of the nonrestoring restorer-of-fertility 1 allele in sugar beet (Beta vulgaris L.)

Theoretical and Applied Genetics volume 129, pages 675–688 (2016)Cite this article

An Erratum to this article was published on 22 April 2017

Abstract

Key message

Only three variants of nonrestoring alleles for sugar beet Rf1 were found from the US maintainer lines which were the selections from a broad range of genetic resources.

Abstract

Cytoplasmic male sterility is widely used for hybrid breeding of sugar beets. Specific genotypes with a nonsterility-inducing cytoplasm and a nonrestoring allele of restorer-of-fertility gene (rf) are called maintainers. The infrequent occurrence of the maintainer genotype evokes the need to diagnose rf alleles. Molecular analysis of Rf1, one of the sugar beet Rfs, revealed a high level of nucleotide sequence diversity, but three variants were tightly associated with maintainer selection in Japan. The question was raised whether this small number of variants would be seen in cases where a wider range of genetic resources was used for maintainer selection. Fifty-seven accessions registered as maintainers in the USDA germplasm collection were characterized in this study. Mitochondrial DNA types (mitotypes) of 551 plants were diagnosed based on minisatellite polymorphism. A mitotype associated with sterility-inducing (S) cytoplasm was identified in 58 plants, indicating S-cytoplasm contamination. The organization of rf1 was investigated by two PCR markers and DNA gel blot analysis. Eight haplotypes were found among the US maintainers, but subsequently two haplotypes were judged as restoring alleles after a test cross and another haplotype was not inherited by the progeny. Nucleotide sequences of rf1 regions in the remaining five haplotypes were compared, and despite the sequence diversity of the gene-flanking regions, the gene-coding regions were identified to be three types. Therefore, there are three rf1 variants in US maintainers, the same number as in the Japanese sugar beet germplasm collection. The implications of having a small repertoire of rf1 variants are discussed.

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Acknowledgments

This work was supported in part by JSPS KAKENHI Grant Number 25292001.

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Affiliations

  1. Research Faculty of Agriculture, Hokkaido University, N-9, W-9, Kita-Ku, Sapporo, 060-8589, Japan

    Takashi Ohgami, Daisuke Uchiyama, Sachiyo Ue, Rika Yui-Kurino, Yu Yoshida, Yoko Kamei & Tomohiko Kubo

  2. Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Memuro, 082-0081, Japan

    Yosuke Kuroda & Kazunori Taguchi

Authors
  1. Takashi Ohgami
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  2. Daisuke Uchiyama
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  3. Sachiyo Ue
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  4. Rika Yui-Kurino
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  5. Yu Yoshida
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  6. Yoko Kamei
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  7. Yosuke Kuroda
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  8. Kazunori Taguchi
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  9. Tomohiko Kubo
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Correspondence to Tomohiko Kubo.

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The authors declare that there is no conflict of interest.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s00122-017-2907-3.

Communicated by B. Hulke.

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Ohgami, T., Uchiyama, D., Ue, S. et al. Identification of molecular variants of the nonrestoring restorer-of-fertility 1 allele in sugar beet (Beta vulgaris L.). Theor Appl Genet 129, 675–688 (2016). https://doi.org/10.1007/s00122-015-2656-0

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  • DOI: https://doi.org/10.1007/s00122-015-2656-0

Keywords

  • Sugar Beet
  • Cytoplasmic Male Sterility
  • Marker Type
  • Fertility Restoration
  • Maintainer Line