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Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group

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The diploid wheat stem rust resistance gene Sr21 confers temperature-sensitive resistance to isolates of the Ug99 group and maps to the middle of the long arm of chromosome 2A m.

Abstract

A race of Puccinia graminis f. sp. tritici, the causal pathogen of stem rust of wheat, known as Ug99, and its variants, are virulent to plants carrying stem rust resistance genes currently deployed in most wheat cultivars worldwide. Therefore, identification, mapping and deployment of effective resistance genes are critical to reduce this threat. Resistance gene Sr21 identified in diploid wheat T. monococcum can be effective against races from the Ug99 race group, but both susceptible and partial resistant reactions have been reported in previous studies. To clarify this conflicting information we screened four monogenic lines with Sr21 and four susceptible controls with 16 Pgt isolates including five isolates of the Ug99 race group under three different temperatures and three different photoperiods. We observed that, temperature influences the interaction between monogenic lines with Sr21 and Ug99 race group isolates, and may be one source of previous inconsistencies. This result indicates that, although Sr21 confers partial resistance against Ug99, its effectiveness can be modulated by environmental conditions and should not be deployed alone. Using two large diploid wheat-mapping populations (total 3,788 F2 plants) we mapped Sr21 approximately 50 cM from the centromere on the long arm of chromosome 2Am within a 0.20 cM interval flanked by sequence-based markers FD527726 and EX594406. The closely linked markers identified in this study will be useful to reduce the T. monococcum segments introgressed into common wheat, accelerate Sr21 deployment in wheat breeding programs, and facilitate the map-based cloning of this gene.

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References

  • Bullrich L, Appendino M, Tranquilli G, Lewis S, Dubcovsky J (2002) Mapping of a thermo-sensitive earliness per se gene on Triticum monococcum chromosome 1Am. Theor Appl Genet 105:585–593

    Article  CAS  PubMed  Google Scholar 

  • Dubcovsky J, Luo M, Dvorak J (1995) Differentiation between homoeologous chromosomes 1A of wheat and 1Am of Triticum monococcum and its recognition by the wheat Ph1 locus. Proc Natl Acad Sci USA 92:6645–6649

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dubcovsky J, Luo M-C, Zhong G-Y, Bransteiter R, Desai A, Kilian A, Kleinhofs A, Dvorak J (1996) Genetic map of diploid wheat, Triticum monococcum L., and its comparison with maps of Hordeum vulgare L. Genetics 143:983–999

    PubMed Central  CAS  PubMed  Google Scholar 

  • Faricelli ME, Valárik M, Dubcovsky J (2010) Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium. Funct Integr Genomics 10:293–306

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Fetch TJ (2007) Virulence of stem rust race TTKS on Canadian wheat cultivars. Can J Plant Pathol 29:441

    Google Scholar 

  • Feuillet C, Travella S, Stein N, Albar L, Nublat A, Keller B (2003) Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat (Triticum aestivum L.) genome. Proc Natl Acad Sci USA 100:15253–15258

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Forsyth F (1956) Interaction of temperature and light on the seedling reaction of McMurachy wheat to race 15B of stem rust. Can J Bot 34:745–749

    Article  Google Scholar 

  • Huang L, Brooks SA, Li W, Fellers JP, Trick HN, Gill BS (2003) Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat. Genetics 164:655–664

    PubMed Central  CAS  PubMed  Google Scholar 

  • Jin Y, Singh R (2006) Resistance in US wheat to recent eastern African isolates of Puccinia graminis f. sp. tritici with virulence to resistance gene Sr31. Plant Dis 90:476–480

    Article  CAS  Google Scholar 

  • Jin Y, Singh R, Ward R, Wanyera R, Kinyua M, Njau P, Fetch T, Pretorius Z, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 91:1096–1099

    Article  Google Scholar 

  • Jin Y, Szabo L, Pretorius Z, Singh R, Ward R, Fetch T Jr (2008) Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 92:923–926

    Article  Google Scholar 

  • Jin Y, Szabo L, Rouse M, Fetch T Jr, Pretorius Z, Wanyera R, Njau P (2009) Detection of virulence to resistance gene Sr36 within the TTKS race lineage of Puccinia graminis f. sp. tritici. Plant Dis 93:367–370

    Article  CAS  Google Scholar 

  • Kellogg EA (2001) Evolutionary history of the grasses. Plant Phys 125:1198–1205

    Article  CAS  Google Scholar 

  • Knott D (1990) Near-isogenic lines of wheat carrying genes for stem rust resistance. Crop Sci 30:901–905

    Article  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Lijavetzky D, Muzzi G, Wicker T, Keller B, Wing R, Dubcovsky J (1999) Construction and characterization of a bacterial artificial chromosome (BAC) library for the A genome of wheat. Genome 42:1176–1182

    Article  CAS  PubMed  Google Scholar 

  • Liu R, Meng J (2003) MapDraw: a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data. Yi chuan = Hereditas/Zhongguo yi chuan xue hui bian ji 25:317–321

  • McIntosh R, Bennett FG (1979) Cytogenetical studies in wheat. IX. Monosomic analyses, telocentric mapping and linkage relationships of genes Sr21, Pm4 and Mle. Aust J Biol Sci 32:115–126

    Google Scholar 

  • Nazari K, Mafi M, Yahyaoui A, Singh RP, Park RF (2009) Detection of wheat stem rust (Puccinia graminis f. sp. tritici) race TTKSK (Ug99) in Iran. Plant Dis 93:317

    Article  Google Scholar 

  • Periyannan S, Moore J, Ayliffe M, Bansal U, Wang X, Huang L, Deal K, Luo M, Kong X, Bariana H (2013) The gene Sr33, an ortholog of barley Mla genes, encodes resistance to wheat stem rust race Ug99. Science 341:786–789

    Article  CAS  PubMed  Google Scholar 

  • Pretorius Z, Singh R, Wagoire W, Payne T (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Dis 84:203

    Article  Google Scholar 

  • Pretorius Z, Bender C, Visser B, Terefe T (2010) First report of a Puccinia graminis f. sp. tritici race virulent to the Sr24 and Sr31 wheat stem rust resistance genes in South Africa. Plant Dis 94:784

    Article  Google Scholar 

  • Pretorius ZA, Szabo LJ, Boshoff WHP, Herselman L, Visser B (2012) First report of a new TTKSF race of wheat stem rust (Puccinia graminis f. sp. tritici) in South Africa and Zimbabwe. Plant Dis 96:590

    Article  Google Scholar 

  • Roelfs A, McVey D (1979) Low infection types produced by Puccinia graminis f. sp. tritici and wheat lines with designated genes for resistance. Phytopathology 69:722–730

    Article  Google Scholar 

  • R Development Core Team. 2011. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/

  • Rouse M, Jin Y (2011a) Genetics of resistance to race TTKSK of Puccinia graminis f. sp. tritici in Triticum monococcum. Phytopathology 101:1418–1423

    Article  CAS  PubMed  Google Scholar 

  • Rouse M, Jin Y (2011b) Stem rust resistance in A-genome diploid relatives of wheat. Plant Dis 95:941–944

    Article  Google Scholar 

  • Rouse MN, Wanyera R, Njau P, Jin Y (2011) Sources of resistance to stem rust race Ug99 in spring wheat germplasm. Plant Dis 95:762–766

    Article  Google Scholar 

  • Rouse MN, Nirmala J, Jin Y, Chao S, Fetch TG Jr, Pretorius ZA, Heibert CW (2014) Characterization of Sr9 h, a wheat stem rust resistance allele effective to Ug99. Theor Appl Genet 127:1681–1688

    Article  CAS  PubMed  Google Scholar 

  • Rowell J (1984) Controlled infection by Puccinia graminis f. sp. tritici under artificial conditions. In: Bushnell WR, Roelfs AP (eds) The cereal rusts, origins, specificity, structure, and physiology, 1st edn. Academic Press, Orlando, pp 292–332

    Google Scholar 

  • Saintenac C, Zhang W, Salcedo A, Rouse MN, Trick HN, Akhunov E, Dubcovsky J (2013) Identification of wheat gene Sr35 that confers resistance to Ug99 stem rust race group. Science 381:783–786

    Article  Google Scholar 

  • Singh RP, Hodson DP, Jin Y, Huerta-Espino J, Kinyua MG, Wanyera R, Njau P, Ward RW (2006) Current status, likely migration and strategies to mitigate the threat to wheat production from race Ug99 (TTKS) of stem rust pathogen. CAB Rev: Perspect Agric Vet Sci Nutr Nat Resour 1:1–13

    Article  CAS  Google Scholar 

  • Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Njau P, Wanyera R, Herrera-Foessel SA, Ward RW (2008) Will stem rust destroy the world’s wheat crop? Adv Agron 98:271–309

    Article  CAS  Google Scholar 

  • Stakman E, Stewart D, Loegering W (1962) Identification of physiologic races of Puccinia graminis var. tritici. USDA. Agricultural Research Service, Washinton, DC, E 617

  • The T (1973) Chromosome location of genes conditioning stem rust resistance transferred from diploid to hexaploid wheat. Nature New Biol 241:256

    Article  CAS  PubMed  Google Scholar 

  • The Brachypodium Consortium Initiative (2010) Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature 463:763–768

    Article  Google Scholar 

  • Todd E, Ronald PC (2000) The evolution of disease resistance genes. Plant Mol Biol 42:195–204

    Article  Google Scholar 

  • Visser B, Herselman L, Park RF, Karaglu H, Bender CM, Pretorius ZA (2011) Characterization of two new Puccinia graminis f. sp. tritici races within the Ug99 lineage in South Africa. Euphytica 179:119–127

    Article  Google Scholar 

  • Watson I, Luig N (1963) The classification of Puccinia graminis var. tritici in relation to breeding resistant varieties. Proc Linn Soc NSW, pp 5–258

  • Watson I, Luig N (1968) Progressive increase in virulence in Puccinia graminis f. sp. tritici. Phytopathology 58:3

    Google Scholar 

  • Zhang W, Olson E, Saintenac C, Rouse M, Abate Z, Jin Y, Akhunov E, Pumphrey M, Dubcovsky J (2010) Genetic maps of stem rust resistance gene Sr35 in diploid and hexaploid wheat. Crop Sci 50:2464–2474

    Article  CAS  Google Scholar 

  • Zhang D, Bowden R, Bai G (2011) A method to linearize Stakman infection type ratings for statistical analysis. Borlaug Global Rust Initiative 2011 Technical workshop, Saint Paul

Download references

Acknowledgments

This project was supported by the Borlaug Global Rust Initiative, by the National Research Initiative Competitive Grants 2011-68002-30029 (Triticeae-CAP) and 2012-67013-19401 from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA), USDA-Agricultural Research Service appropriated project 3640-21220-021-00, USDA National Plant Disease Recovery System, and by support from the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. We thank Mariana Padilla for excellent technical support.

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The authors declare that they have no conflict of interest.

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Correspondence to Jorge Dubcovsky.

Additional information

Communicated by Evans Lagudah.

S. Chen, M. N. Rouse and W. Zhang contributed equally to this work.

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122_2015_2460_MOESM1_ESM.pdf

Supplementary material 1 (PDF 105 kb) Supplementary Table S1 Seedling infection types of wheat lines with and without Sr21 to diverse isolates (and corresponding races) of Puccinia graminis f. sp. tritici at various temperatures and photoperiods

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Chen, S., Rouse, M.N., Zhang, W. et al. Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group. Theor Appl Genet 128, 645–656 (2015). https://doi.org/10.1007/s00122-015-2460-x

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