Skip to main content
Log in

Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript


Key message

Sclerotinia resistance was transferred into rapeseed from a wild relative of Brassica oleracea ( B. incana ) using hexaploids derived from crosses between B. incana and rapeseed as a bridge.


A high level of resistance against Sclerotinia sclerotiorum has been documented in wild Brassica oleracea, but not in cultivated rapeseed (Brassica napus). To transfer sclerotinia resistance from a wild relative into rapeseed, a strategy was proposed using hexaploids (AACCCC) derived from crosses between the wild B. oleracea-related B. incana genotype ‘C01’ and the Chinese rapeseed variety ‘Zhongshuang 9’ as a bridge. Progenies (BC1F1) generated by backcrossing the hexaploid to ‘Zhongshuang 9’ could be generated with a high crossability (average 18.3 seeds per pod). Seventy-three individuals in BC1F1 were firstly screened for resistance with five molecular markers linked to the major resistance QTL on chromosome C09 in ‘C01’, and 11 individuals harboring resistance loci were selected to develop vegetative clones. Of these, five exhibited significantly higher resistance than ‘Zhongshuang 9’ and the most resistant individual was chosen to develop the BC1F2 progeny. Finally, five individual genotypes with nearly twofold higher resistance than ‘Zhongshuang 9’ were found among 100 BC1F2 individuals by using marker-assisted selection and resistance evaluation. Hereof, one rapeseed-type individual with 38 chromosomes and good self-fertility (15.0 ± 3.56 seeds/pod) was identified. Our results indicate that the proposed strategy is effective for transferring sclerotinia resistance from a wild relative of B. oleracea into rapeseed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others


  • Bradley C, Hamey H (2005) Canola disease situation in North Dakota, USA, 1993–2004. Proceedings of 4th Australian Research Assembly on Brassicas, pp 33–34. Port Lincoln, Australia

  • Cui C, Ge X, Gautam M, Kang L, Li Z (2012) Cytoplasmic and genomic effects on meiotic pairing in Brassica hybrids and allotetraploids from pair crosses of three cultivated diploids. Genetics 191:725–738

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • del Río LE, Bradley CA, Henson RA, Endres GJ, Hanson BK, Mckay K, Halvorson M, Porter PM, Le Gare DG, Lamey HA (2007) Impact of Sclerotinia stem rot on yield of canola. Plant Dis 91:191–194

    Article  Google Scholar 

  • Diederichsen E, Sacristan MD (1996) Disease response of resynthesized Brassica napus L. lines carrying different combinations of resistance to Plasmodiophora brassicae Wor. Plant Breed 115:5–10

    Article  Google Scholar 

  • Ding YJ, Mei JQ, Li QF, Liu Y, Wan HF, Wang L, Becker HC, Qian W (2013) Improvement of Sclerotinia sclerotiorum resistance in Brassica napus by using B. oleracea. Genet Resour Crop Evol 60:1615–1619

    Article  Google Scholar 

  • Falak I, Primomo V, Tulsieram L (2011) Mapping of QTLs associated with Sclerotinia Stem Rot resistance in Spring Canola Brassica napus. 13th International Rapeseed Congress, pp 772–774. Prague, Czech

  • Friedt W, Snowdon RJ (2010) Oilseed rape. In: Vollmann J, Rajcan I (eds) Handbook of plant breeding, Oil crops, vol 4. Springer, New York, p 91

    Google Scholar 

  • Koch S, Dunker S, Kleinhenz B, Rohrig M, von Tiedemann A (2007) Crop loss-related forecasting model for Sclerotinia stem rot in winter oilseed rape. Phytopathology 97:1186–1194

    Article  CAS  PubMed  Google Scholar 

  • Li Y, Chen J, Bennett R, Kiddle G, Wallsgrove R, Huang Y, He Y (1999) Breeding, inheritance, and biochemical studies on Brassica napus cv. Zhougyou 821: tolerance to Sclerotinia sclerotiorum (stem rot). Proceedings of the 10th International Rapeseed Congress, pp 61, Canberra, Australia

  • Li M, Qian W, Meng J, Li Z (2004) Construction of novel Brassica napus genotypes through chromosomal substitution and elimination using interploid species hybridization. Chromosome Res 12:417–426

    Article  PubMed  Google Scholar 

  • Li QF, Mei JQ, Zhang YJ, Li JN, Ge XH, Li ZY, Qian W (2013) A large-scale introgression of genomic components of Brassica rapa into B. napus by the bridge of hexaploid derived from hybridization between B. napus and B. oleracea. Theor Appl Genet 126:2073–2080

    Article  CAS  PubMed  Google Scholar 

  • Mei J, Qian L, Disi JO, Yang X, Li Q, Li J, Frauen M, Cai D, Qian W (2011) Identification of resistant sources against Sclerotinia sclerotiorum in Brassica crops with emphasis on B. oleracea. Euphytica 177:393–400

    Article  Google Scholar 

  • Mei JQ, Wei DY, Disi JO, Ding YJ, Liu Y, Qian W (2012) Screening resistance against Sclerotinia sclerotiorum in Brassica crops with use of detached stem assay under controlled environment. Eur J Plant Pathol 134:599–604

    Article  Google Scholar 

  • Mei JQ, Ding YJ, Lu K, Wei DY, Liu Y, Disi JO, Li JN, Liu LZ, Liu SY, McKay J, Qian W (2013) Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea. Theor Appl Genet 126:549–556

    Article  CAS  PubMed  Google Scholar 

  • Nagaharu U (1935) Genomic analysis in Brassica with special reference to the experimental formation of B. napus and peculiar bode of fertilization. J Jpn Bot 7:389–452

    Google Scholar 

  • SAS Institute (1992) SAS technical report. SAS statistics software: changes and enhancements. Release 6.07

  • Wang H, Liu G, Zheng Y, Wang X, Yang Q (2004) Breeding of the Brassica napus cultivar Zhongshuang 9 with high resistance to Sclerotinia sclerotiorum and dynamics of its important defense enzyme activity. Sci Agri Sin 37(1):23–28

    CAS  Google Scholar 

  • Wen J, Tu JX, Li ZY, Fu TD, Ma CZ, Shen JX (2008) Improving ovary and embryo culture techniques for efficient resynthesis of Brassica napus from reciprocal crosses between yellow-seeded diploids B. rapa and B. oleracea. Euphytica 162:81–89

    Article  Google Scholar 

  • Yu B, Liu P, Hong D, He Q, Yang G (2010) Improvement of Sclerotinia resistance of a Polima CMS restorer line of rapeseed via phenotypic selection, marker-assisted background selection and microspore culture

Download references


We thank Elke Diederichsen (Free University of Berlin, Germany) for providing the S. sclerotiorum isolate, and Peter M. Gresshoff (the University of Queensland, Australia) for correcting the manuscript. This study was financially supported by “Forschungsund Entwicklungsfonds Raps’’ in Germany, the 973 Program (2015CB150201), the Key Projects in the National Science and Technology (2014BAD01B07), NSFC (31401861, 31401411, 31171585) and the Fundamental Research Funds for the Central Universities (XDJK2013A013, XDJK2014A015, SWU113106 and XDJK2014B036) in China.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations


Corresponding authors

Correspondence to Wei Qian or Wolfgang Friedt.

Additional information

Communicated by Richard G.F. Visser.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mei, J., Liu, Y., Wei, D. et al. Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step. Theor Appl Genet 128, 639–644 (2015).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: