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Markers for seedling and adult plant crown rot resistance in four partially resistant bread wheat sources

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QTL identified for seedling and adult plant crown rot resistance in four partially resistant hexaploid wheat sources. PCR-based markers identified for use in marker-assisted selection.

Abstract

Crown rot, caused by Fusarium pseudograminearum, is an important disease of wheat in many wheat-growing regions globally. Complete resistance to infection by F. pseudograminearum has not been observed in a wheat host, but germplasm with partial resistance to this pathogen has been identified. The partially resistant wheat hexaploid germplasm sources 2-49, Sunco, IRN497 and CPI133817 were investigated in both seedling and adult plant field trials to identify markers associated with the resistance which could be used in marker-assisted selection programs. Thirteen different quantitative trait loci (QTL) conditioning crown rot resistance were identified in the four different sources. Some QTL were only observed in seedling trials whereas others appeared to be adult plant specific. For example while the QTL on chromosomes 1AS, 1BS, and 4BS contributed by 2-49 and on 2BS contributed by Sunco were detected in both seedling and field trials, the QTL on 1DL present in 2-49 and the QTL on 3BL in IRN497 were only detected in seedling trials. Genetic correlations between field trials of the same population were strong, as were correlations between seedling trials of the same population. Low to moderate correlations were observed between seedling and field trials. Flanking markers, most of which are less than 10 cM apart, have now been identified for each of the regions associated with crown rot resistance.

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References

  1. Backhouse D, Abubakar AA, Burgess LW, Dennis JI, Hollaway GJ, Wildermuth GB, Wallwork H, Henry FJ (2004) Survey of Fusarium species associated with crown rot of wheat and barley in eastern Australia. Australas Plant Pathol 33:255–261

    Article  Google Scholar 

  2. Bovill WD, Ma W, Ritter K, Collard BCY, Davis M, Wildermuth GB, Sutherland MW (2006) Identification of novel QTL for resistance to crown rot in the doubled haploid wheat population ‘W21MMT70’ × ‘Mendos’. Plant Breed 125(6):538–543

    Article  CAS  Google Scholar 

  3. Bovill WD, Horne M, Herde D, Davis M, Wildermuth GB, Sutherland MW (2010) Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum). Theor Appl Genet 121(1):127–136

    Article  CAS  PubMed  Google Scholar 

  4. Bureau of Meteorology, Australian Government. http://www.bom.gov.au/jsp/ncc/cdio/weatherData/av?p_nccObsCode=139&p_display_type=dataFile&p_startYear=&p_c=&p_stn_num=041529. Accessed 14 Aug 2014

  5. Burgess LW, Backhouse D, Summerell BA, Swan LJ (2001) Crown rot of wheat. In: Summerell BA, Leslie JF, Backhouse D, Bryden WL, Burgess LW (eds) Fusarium. American Phytopathological Society, St. Paul

    Google Scholar 

  6. Butler DG, Cullis BR, Gilmour AR, Gogel BJ (2009) ASReml-R reference manual, release 3, technical report. Department of Primary Industries, Queensland

  7. Collard BCY, Grams RA, Bovill WD, Percy CD, Jolley R, Lehmensiek A, Wildermuth G, Sutherland MW (2005) Development of molecular markers for crown rot resistance in wheat: mapping of QTLs for seedling resistance in a ‘2-49’ × ‘Janz’ population. Plant Breed 124(6):532–537

    Article  CAS  Google Scholar 

  8. Collard BCY, Jolley R, Bovill WD, Grams RA, Wildermuth GB, Sutherland MW (2006) Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line ‘2-49’. Aust J Agric Res 57(9):967–973

    Article  CAS  Google Scholar 

  9. Cullis BR, Smith A, Coombes N (2006) On the design of early generation variety trials with correlated data. J Agric Biol Environ Stat 11:381–393

    Article  Google Scholar 

  10. Dodman RL, Wildermuth GB (1987) Inoculation methods for assessing resistance in wheat to crown rot caused by Fusarium graminearum Group 1. Aust J Agric Res 38:473–486

    Google Scholar 

  11. Gilmour AR, Cullis BR, Verbyla AP (1997) Accounting for natural and extraneous variation in the analysis of field experiments. J Agric Biol Environ Stat 2:269–293

    Article  Google Scholar 

  12. Knight NL, Sutherland MW (2013) Histopathological assessment of wheat seedling tissues infected by Fusarium pseudograminearum. Plant Pathol 62(3):679–687

    Article  CAS  Google Scholar 

  13. Knight NL, Sutherland MW, Martin A, Herde DJ (2012) Assessment of infection by Fusarium pseudograminearum in wheat seedling tissues using quantitative PCR and a Visual Discoloration Scale. Plant Dis 96(11):1661–1669

    Article  CAS  Google Scholar 

  14. Li HB, Xie GQ, Ma J, Liu GR, Wen SM, Ban T, Chakraborty S, Liu CJ (2010) Genetic relationships between resistances to Fusarium head blight and crown rot in bread wheat (Triticum aestivum L.). Theor Appl Genet 121(5):941–950

    Article  PubMed  Google Scholar 

  15. Ma J, Li HB, Zhang CY, Yang XM, Liu YX, Yan GJ, Liu CJ (2010) Identification and validation of a major QTL conferring crown rot resistance in hexaploid wheat. Theor Appl Genet 120(6):1119–1128

    Article  CAS  PubMed  Google Scholar 

  16. Patterson HD, Thompson R (1971) Recovery of interblock information when block sizes are unequal. Biometrika 31:100–109

    Google Scholar 

  17. Percy CD, Wildermuth GB, Sutherland MW (2012) Symptom development proceeds at different rates in susceptible and partially resistant cereal seedlings infected with Fusarium pseudograminearum. Australas Plant Pathol 41(6):621–631

    Article  Google Scholar 

  18. Poole GJ, Smiley RW, Paulitz TC, Walker CA, Carter AH, See DR, Garland-Campbell K (2012) Identification of quantitative trait loci (QTL) for resistance to Fusarium crown rot (Fusarium pseudograminearum) in multiple assay environments in the Pacific Northwestern US. Theor Appl Genet 125(1):91–107

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. R Development Core Team (2013) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org

  20. Smiley RW, Gourlie JA, Easley SA, Patterson L-M, Whittaker RG (2005) Crop damage estimates for crown rot of wheat and barley in the Pacific Northwest. Plant Dis 89:595–604

    Article  Google Scholar 

  21. Smith AB, Cullis BR, Thompson R (2001) Analyzing variety by environment data using multiplicative mixed models and adjustments for spatial field trends. Biometrics 57:1138–1147

    Article  CAS  PubMed  Google Scholar 

  22. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  CAS  PubMed  Google Scholar 

  23. Wallwork H, Butt M, Cheong JPE, Williams KJ (2004) Resistance to crown rot in wheat identified through an improved method for screening adult plants. Australas Plant Pathol 33(1):1–7

    Article  Google Scholar 

  24. Wildermuth GB, McNamara RB (1994) Testing wheat seedlings for resistance to crown rot caused by Fusarium graminearum Group 1. Plant Dis 78:949–953

    Article  Google Scholar 

  25. Wildermuth GB, Morgan JM (2004) Genotypic differences in partial resistance to crown rot caused by Fusarium pseudograminearum in relation to an osmoregulation gene in wheat. Australas Plant Pathol 33(1):121–123

    Article  CAS  Google Scholar 

  26. Wildermuth GB, McNamara RB, Quick JS (2001) Crown depth and susceptibility to crown rot in wheat. Euphytica 122(2):397–405

    Article  Google Scholar 

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Acknowledgments

This research was funded by the Grains Research and Development Corporation.

Conflict of interest

The authors declare that we have no conflict of interest.

Ethical standards

The authors declare that all experiments complied with the ethical standards in the country in which they were performed.

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Correspondence to A. Martin.

Additional information

Communicated by Xianchun Xia.

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Martin, A., Bovill, W.D., Percy, C.D. et al. Markers for seedling and adult plant crown rot resistance in four partially resistant bread wheat sources. Theor Appl Genet 128, 377–385 (2015). https://doi.org/10.1007/s00122-014-2437-1

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Keywords

  • Quantitative Trait Locus
  • Field Trial
  • Doubled Haploid
  • Quantitative Trait Locus Region
  • Flank Marker