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Characterization of Sr9h, a wheat stem rust resistance allele effective to Ug99

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Abstract

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Wheat stem rust resistance gene SrWeb is an allele at the Sr9 locus that confers resistance to Ug99.

Abstract

Race TTKSK (Ug99) of Puccinia graminis f. sp. tritici, the causal fungus of stem rust, threatens global wheat production because of its broad virulence to current wheat cultivars. A recently identified Ug99 resistance gene from cultivar Webster, temporarily designated as SrWeb, mapped near the stem rust resistance gene locus Sr9. We determined that SrWeb is also present in Ug99 resistant cultivar Gabo 56 by comparative mapping and an allelism test. Analysis of resistance in a population segregating for both Sr9e and SrWeb demonstrated that SrWeb is an allele at the Sr9 locus, which subsequently was designated as Sr9h. Webster and Gabo 56 were susceptible to the Ug99-related race TTKSF+ from South Africa. Race TTKSF+ possesses unique virulence to uncharacterized Ug99 resistance in cultivar Matlabas. This result validated that resistance to Ug99 in Webster and Gabo 56 is conferred by the same gene: Sr9h. The emergence of pathogen virulence to several resistance genes that are effective to the original Ug99 race TTKSK, including Sr9h, suggests that resistance genes should be used in combinations in order to increase resistance durability.

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Acknowledgments

This research was part of the Durable Rust Resistance in Wheat project administrated by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International Development. Additional support was received from the United States Department of Agriculture, Agricultural Research Service National Plant Disease Recovery Plan. We acknowledge the Computational Genetics Laboratory of the University of Minnesota Supercomputing Institute for computational support. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products and vendors that might also be suitable.

Conflict of interest

The authors declare that there are no conflict of interest.

Ethical standard

The authors declare that the experiments comply with the current laws of the countries in which the experiments were performed.

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Authors and Affiliations

  1. Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), 1551 Lindig Street, St. Paul, MN, USA

    Matthew N. Rouse, Jayaveeramuthu Nirmala & Yue Jin

  2. Department of Plant Pathology, University of Minnesota, 1991 Upper Buford Circle, 495 Borlaug Hall, St. Paul, MN, 55108, USA

    Matthew N. Rouse & Yue Jin

  3. Biosciences Research Laboratory, USDA-ARS, 1605 Albrecht Blvd. N., Fargo, ND, 58102, USA

    Shiaoman Chao

  4. Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Road, Winnipeg, MB, R3T 2M9, Canada

    Thomas G. Fetch Jr. & Colin W. Hiebert

  5. Department of Plant Sciences, University of the Free State, Bloemfontein, 9300, South Africa

    Zacharias A. Pretorius

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  1. Matthew N. Rouse
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  2. Jayaveeramuthu Nirmala
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  3. Yue Jin
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  5. Thomas G. Fetch Jr.
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  7. Colin W. Hiebert
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Correspondence to Matthew N. Rouse.

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Communicated by Evans Lagudah.

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Rouse, M.N., Nirmala, J., Jin, Y. et al. Characterization of Sr9h, a wheat stem rust resistance allele effective to Ug99. Theor Appl Genet 127, 1681–1688 (2014). https://doi.org/10.1007/s00122-014-2330-y

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