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Comparative QTL analysis of root lesion nematode resistance in barley

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Abstract

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This study demonstrates for the first time that resistance to different root lesion nematodes ( P. neglectus and P. penetrans ) is controlled by a common QTL. A major resistance QTL ( Rlnnp6H ) has been mapped to chromosome 6H using two independent barley populations.

Abstract

Root lesion nematodes (Pratylenchus spp.) are important pests in cereal production worldwide. We selected two doubled haploid populations of barley (Igri × Franka and Uschi × HHOR 3073) and infected them with Pratylenchus penetrans and Pratylenchus neglectus. Nematode multiplication rates were measured 7 or 10 weeks after infection. In both populations, continuous phenotypic variations for nematode multiplication rates were detected indicating a quantitative inheritance of resistance. In the Igri × Franka population, four P. penetrans resistance QTLs were mapped with 857 molecular markers on four linkage groups (2H, 5H, 6H and 7H). In the Uschi × HHOR 3073 population, eleven resistance QTLs (P. penetrans and P. neglectus) were mapped with 646 molecular markers on linkage groups 1H, 3H, 4H, 5H, 6H and 7H. A major resistance QTL named Rlnnp6H (LOD score 6.42–11.19) with a large phenotypic effect (27.5–36.6 %) for both pests was mapped in both populations to chromosome 6H. Another resistance QTL for both pests was mapped on linkage group 5H (Igri × Franka population). These data provide first evidence for common resistance mechanisms against different root lesion nematode species. The molecular markers are a powerful tool for the selection of resistant barley lines among segregating populations because resistance tests are time consuming and laborious.

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Acknowledgments

The authors gratefully acknowledge Bärbel Wohnsen, Cay Kruse, Ines Schütt and Claudia Weißleder for their excellent technical assistance. This work was financially supported by the BLE grant No. 28-1-41.030-06. A. Galal is supported by a scholarship from the Egyptian Ministry of Higher Education and the Faculty of Agriculture, Kafrelsheikh University, Egypt.

Conflict of interest

I declare that there have been no involvements that might raise the question of bias in the work reported or in the conclusions, implications, or opinions stated.

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Author notes

  1. Shiveta Sharma & Shailendra Sharma

    Present address: 1101 Institute for Genomic Biology, University of Illinois, 1206 W. Gregory Avenue, Urbana, IL, 61801, USA

Authors and Affiliations

  1. Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany

    Ahmed Galal, Shiveta Sharma, Salah Fatouh Abou-Elwafa, Shailendra Sharma, Friedrich Kopisch-Obuch & Christian Jung

  2. Nordsaat Saatzucht GmbH, Hofweg 8, 23899, Segrahn, Germany

    Eberhard Laubach

  3. Institute for Resistance Research and Stress Tolerance, Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Dragan Perovic & Frank Ordon

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Correspondence to Christian Jung.

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Communicated by A. Graner.

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Galal, A., Sharma, S., Abou-Elwafa, S.F. et al. Comparative QTL analysis of root lesion nematode resistance in barley. Theor Appl Genet 127, 1399–1407 (2014). https://doi.org/10.1007/s00122-014-2307-x

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  • DOI: https://doi.org/10.1007/s00122-014-2307-x

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