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Quantitative trait loci that control the oil content variation of rapeseed (Brassica napus L.)

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This report describes an integrative analysis of seed-oil-content quantitative trait loci (QTL) in Brassica napus , using a high-density genetic map to align QTL among different populations.

Abstract

Rapeseed (Brassica napus) is an important source of edible oil and sustainable energy. Given the challenge involved in using only a few genes to substantially increase the oil content of rapeseed without affecting the fatty acid composition, exploitation of a greater number of genetic loci that regulate the oil content variation among rapeseed germplasm is of fundamental importance. In this study, we investigated variation in the seed-oil content among two related genetic populations of Brassica napus, the TN double-haploid population and its derivative reconstructed-F2 population. Each population was grown in multiple experiments under different environmental conditions. Mapping of quantitative trait loci (QTL) identified 41 QTL in the TN populations. Furthermore, of the 20 pairs of epistatic interaction loci detected, approximately one-third were located within the QTL intervals. The use of common markers on different genetic maps and the TN genetic map as a reference enabled us to project QTL from an additional three genetic populations onto the TN genetic map. In summary, we used the TN genetic map of the B. napus genome to identify 46 distinct QTL regions that control seed-oil content on 16 of the 19 linkage groups of B. napus. Of these, 18 were each detected in multiple populations. The present results are of value for ongoing efforts to breed rapeseed with high oil content, and alignment of the QTL makes an important contribution to the development of an integrative system for genetic studies of rapeseed.

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Acknowledgments

This work was supported by the National Basic Research and Development Program (2006CB101600), P. R. China.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Congcong Jiang, Jiaqin Shi, Ruiyuan Li, Yan Long & Jinling Meng

  2. Hybrid Rapeseed Research Centre of Shaanxi, Dali, 715105, People’s Republic of China

    Hao Wang & Dianrong Li

  3. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China

    Jianyi Zhao

Authors
  1. Congcong Jiang
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  2. Jiaqin Shi
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  3. Ruiyuan Li
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  4. Yan Long
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  5. Hao Wang
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  6. Dianrong Li
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  7. Jianyi Zhao
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  8. Jinling Meng
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Corresponding author

Correspondence to Jinling Meng.

Additional information

Communicated by C. F. Quiros.

Electronic supplementary material

Below is the link to the electronic supplementary material.

122_2014_2271_MOESM1_ESM.xlsx

Supplementary material 1. Temporal and spatial details of the 15 independent experiments that based on TN population (XLSX 10 kb)

Supplementary material 2. The TN genetic map that used in this study (XLSX 50 kb)

Supplementary material 3. Seed oil content QTL that identified in TN population (XLSX 20 kb)

122_2014_2271_MOESM4_ESM.xlsx

Supplementary material 4. Projected locations on TN genetic map of the QTL that identified in other populations (XLSX 10 kb)

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Jiang, C., Shi, J., Li, R. et al. Quantitative trait loci that control the oil content variation of rapeseed (Brassica napus L.). Theor Appl Genet 127, 957–968 (2014). https://doi.org/10.1007/s00122-014-2271-5

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  • DOI: https://doi.org/10.1007/s00122-014-2271-5

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