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Genetic analysis of single-locus and epistatic QTLs for seed traits in an adapted × nuña RIL population of common bean (Phaseolus vulgaris L.)

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Abstract

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The QTLs analyses here reported demonstrate the significant role of both individual additive and epistatic effects in the genetic control of seed quality traits in the Andean common bean.

Abstract

Common bean shows considerable variability in seed size and coat color, which are important agronomic traits determining farmer and consumer acceptability. Therefore, strategies must be devised to improve the genetic base of cultivated germplasm with new alleles that would contribute positively to breeding programs. For that purpose, a population of 185 recombinant inbred lines derived from an Andean intra-gene pool cross, involving an adapted common bean (PMB0225 parent) and an exotic nuña bean (PHA1037 parent), was evaluated under six different—short and long-day—environmental conditions for seed dimension, weight, color, and brightness traits, as well as the number of seed per pod. A multi-environment Quantitative Trait Loci (QTL) analysis was carried out and 59 QTLs were mapped on all linkage groups, 18 of which had only individual additive effects, while 27 showed only epistatic effects and 14 had both individual additive and epistatic effects. Multivariate models that included significant QTL explained from 8 to 68  % and 2 to 15 % of the additive and epistatic effects, respectively. Most of these QTLs were consistent over environment, though interactions between QTLs and environments were also detected. Despite this, QTLs with differential effect on long-day and short-day environments were not found. QTLs identified were positioned in cluster, suggesting that either pleiotropic QTLs control several traits or tightly linked QTLs for different traits map together in the same genomic regions. Overall, our results show that digenic epistatic interactions clearly play an important role in the genetic control of seed quality traits in the Andean common bean.

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Acknowledgments

The research has been funded by grant AGL2011-25562 from the Ministerio de Economía y Competitividad (Spain). We also thank Campus de Excelencia Internacional Agroalimentario (CeiA3) for supporting in part this work.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

Experiments conducted for this study comply with the current laws of Spain.

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Authors and Affiliations

  1. Centro de Investigación en Biotecnología Agroalimentaria (BITAL). Campus de Excelencia Internacional Agroalimentario, CeiA3, Universidad de Almería, 04120, Almería, Spain

    Fernando J. Yuste-Lisbona, Carmen Capel, Manuel García-Alcázar, Juan Capel & Rafael Lozano

  2. Grupo de Biología de Agrosistemas, Departamento de Recursos Fitogenéticos, Misión Biológica de Galicia-CSIC, P.O. Box 28, 36080, Pontevedra, Spain

    Ana M. González, Antonio M. De Ron & Marta Santalla

  3. Department Biología y Geología, Edificio CITE II-B, Universidad de Almería, Carretera de Sacramento s/n, 04120, Almería, Spain

    Rafael Lozano

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  1. Fernando J. Yuste-Lisbona
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  2. Ana M. González
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  3. Carmen Capel
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  4. Manuel García-Alcázar
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  5. Juan Capel
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  6. Antonio M. De Ron
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  7. Rafael Lozano
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  8. Marta Santalla
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Corresponding author

Correspondence to Rafael Lozano.

Additional information

Communicated by B. Diers.

F. J. Yuste-Lisbona and A. M. González should be regarded as joint first authors.

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Yuste-Lisbona, F.J., González, A.M., Capel, C. et al. Genetic analysis of single-locus and epistatic QTLs for seed traits in an adapted × nuña RIL population of common bean (Phaseolus vulgaris L.). Theor Appl Genet 127, 897–912 (2014). https://doi.org/10.1007/s00122-014-2265-3

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