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Theoretical and Applied Genetics

, Volume 127, Issue 1, pp 167–177 | Cite as

Mapping and genomic targeting of the major leaf shape gene (L) in Upland cotton (Gossypium hirsutum L.)

  • Ryan J. Andres
  • Daryl T. Bowman
  • Baljinder Kaur
  • Vasu KuraparthyEmail author
Original Paper

Abstract

Key message

A major leaf shape locus (L) was mapped with molecular markers and genomically targeted to a small region in the D-genome of cotton. By using expression analysis and candidate gene mapping, two LMI1 -like genes are identified as possible candidates for leaf shape trait in cotton.

Abstract

Leaf shape in cotton is an important trait that influences yield, flowering rates, disease resistance, lint trash, and the efficacy of foliar chemical application. The leaves of okra leaf cotton display a significantly enhanced lobing pattern, as well as ectopic outgrowths along the lobe margins when compared with normal leaf cotton. These phenotypes are the hallmark characteristics of mutations in various known modifiers of leaf shape that culminate in the mis/over-expression of Class I KNOX genes. To better understand the molecular and genetic processes underlying leaf shape in cotton, a normal leaf accession (PI607650) was crossed to an okra leaf breeding line (NC05AZ21). An F2 population of 236 individuals confirmed the incompletely dominant single gene nature of the okra leaf shape trait in Gossypium hirsutum L. Molecular mapping with simple sequence repeat markers localized the leaf shape gene to 5.4 cM interval in the distal region of the short arm of chromosome 15. Orthologous mapping of the closely linked markers with the sequenced diploid D-genome (Gossypium raimondii) tentatively resolved the leaf shape locus to a small genomic region. RT-PCR-based expression analysis and candidate gene mapping indicated that the okra leaf shape gene (L o ) in cotton might be an upstream regulator of Class I KNOX genes. The linked molecular markers and delineated genomic region in the sequenced diploid D-genome will assist in the future high-resolution mapping and map-based cloning of the leaf shape gene in cotton.

Keywords

Simple Sequence Repeat Marker Leaf Shape Upland Cotton Normal Leaf Restriction Fragment Length Polymorphism Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Cotton Incorporated for supporting this work through its core research and doctoral fellowship programs. We thank Kim Howell, Jared Smith, and Sharon Williamson at the USDA Eastern Regional Small Grains Genotyping Laboratory for assistance in capillary-based analysis of molecular markers. We also thank Rich Tuttle for supplying primers for GAPDH-positive control used in the expression analysis.

Conflict of interest

The authors declare that there are no conflicts of interest in the reported research.

Ethical standards

The authors note that this research was performed and reported in accordance with ethical standards of the scientific conduct.

Supplementary material

122_2013_2208_MOESM1_ESM.docx (33 kb)
Supplementary material 1 (DOCX 32 kb)

References

  1. Blenda A, Fang DD, Rami JF, Garsmeur O, Luo F, Lacape JM (2012) A high density consensus genetic map of tetraploid cotton that integrates multiple component maps through molecular marker redundancy check. PLoS One 7:1–16CrossRefGoogle Scholar
  2. Chen ZJ, Scheffler BE, Dennis E, Triplett BA, Zhang T, Guo W, Chen X, Stelly DM, Rabinowicz PD, Town CD, Arioli T, Brubaker C, Cantrell RG, Lacape JM, Ulloa M, Chee P, Gingle AR, Haigler CH, Percy R, Saha S, Wilkins T, Wright RJ, Deynze AV, Zhu Y, Yu S, Abdurakhmonov I, Katageri I, Kumar PA, ur-Rhaman M, Zafar Y, Yu JZ, Kohel RJ, Wendel JF, Paterson AH (2007) Toward sequencing cotton (Gossypium) genomes. Plant Physiol 145:1303–1310PubMedCentralPubMedCrossRefGoogle Scholar
  3. Dolan L, Poethig RS (1991) Genetic analysis of leaf development in cotton. Development 1:39–46Google Scholar
  4. Endrizzi JE, Brown MS (1964) Identification of monosomes for six chromosomes in Gossypium hirsutum. Am J Bot 51:117–120CrossRefGoogle Scholar
  5. Endrizzi JE, Kohel RJ (1966) Use of telosomes in mapping three chromosomes in cotton. Genetics 54:535–550PubMedGoogle Scholar
  6. Faris JD, Fellers JP, Brooks SA, Gill BS (2003) A bacterial artificial chromosome contig spanning the major domestication locus Q in wheat and identification of a candidate gene. Genetics 164:311–321PubMedGoogle Scholar
  7. Gill KS, Gill BS, Endo TR, Boyko EV (1996) Identification and high-density mapping of gene rich regions in chromosome group 5 of wheat. Genetics 143:1001–1012PubMedGoogle Scholar
  8. Ha CM, Jun JH, Nam HG, Fletcher JC (2004) BLADE-ON-PETIOLE1 encodes BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana. Plant Cell Physiol 45(10):1361–1370PubMedCrossRefGoogle Scholar
  9. Hay A, Tsiantis M (2010) KNOX genes: versatile regulators of plant development and diversity. Development 137:3153–3165PubMedCrossRefGoogle Scholar
  10. Janssen BJ, Lund L, Sinha N (1998) Overexpression of a homeobox gene, LeT6, reveals indeterminate features in the tomato compound leaf. Plant Physiol 117:771–786PubMedCentralPubMedCrossRefGoogle Scholar
  11. Jiang C, Wright RJ, Woo SS, DelMonte TA, Paterson AH (2000) QTL analysis of leaf morphology in tetraploid Gossypium (cotton). Theor Appl Genet 100:409–418CrossRefGoogle Scholar
  12. Jones JE (1982) The present state of the art and science of cotton breeding for leaf-morphological types. Proceedings of the Beltwide Cotton Production Research Conferences, National Cotton Council of America, Memphis, TN pp 93–99Google Scholar
  13. Kuraparthy V, Bowman DT, Jones DC (2013) Registration of NC05AZ21 okra-leaf and NC05-11 normal-leaf Upland cotton germplasm. J Plant Registrat 7:334–338CrossRefGoogle Scholar
  14. Lin Z, Li X, Shannon LM, Yeh CT, Wang ML, Bai G, Peng Z, Li J, Trick HN, Clemente TE, Doebley J, Schnable PS, Tuinstra MR, Tesso TT, White F, Yu J (2012) Parallel domestication of the Shattering1 genes in cereals. Nat Genet 44:720–724PubMedCentralPubMedCrossRefGoogle Scholar
  15. Lincoln C, Long J, Yamaguchi J, Serikawa K, Hake S (1994) A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants. Plant Cell 6:1859–1876PubMedCentralPubMedGoogle Scholar
  16. Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436PubMedCrossRefGoogle Scholar
  17. Meredith WR (1984) Influence of leaf morphology on lint yield of cotton-enhancement by the sub okra trait. Crop Sci 24:855–857CrossRefGoogle Scholar
  18. Rahman HU, Bibi A, Latif M (2005) Okra-leaf accessions of the upland cotton (Gossypium hirsutum L.): genetic variability in agronomic and fibre traits. J Appl Genet 46:149–155Google Scholar
  19. Rong J, Abbey C, Bowers JE, Brubaker CL, Chang C, Chee PW, Delmonte TA, Ding X, Garza JJ, Marler BS, Park C, Pierce GJ, Rainey KM, Rastogi VK, Schulze SR, Trolinder NL, Wendel JF, Wilkins TA, Williams-Coplin TD, Wing RA, Wright RJ, Zhao X, Zhu L, Paterson AH (2004) A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics 166:389–417PubMedCrossRefGoogle Scholar
  20. Rosin FM, Hark JK, Horner HT, Davies PJ, Hannapel DJ (2003) Overexpression of a Knotted-like homeobox gene of potato alters vegetative development by decreasing gibberellin accumulation. Plant Physiol 132:1106–1117CrossRefGoogle Scholar
  21. Saddic LA, Huvermann B, Bezhani S, Su Y, Winter CM, Kwon CS, Collum RP, Wagner D (2006) The LEAFY target LMI1 is a meristem identity regulator and acts together with LEAFY to regulate expression of CAULIFLOWER. Development 133:1673–1682PubMedCrossRefGoogle Scholar
  22. Schuelke M (2000) An economic method of the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  23. Song XL, Guo WZ, Han ZG, Zhang TZ (2005) Quantitative trait loci mapping of leaf morphological traits and chlorophyll content in cultivated tetraploid cotton. J Integr Plant Biol 47:1382–1390CrossRefGoogle Scholar
  24. Tanaka-Ueguchi M, Itoh H, Oyama N, Koshioka M, Matsuoka M (1998) Over-expression of a tobacco homeobox gene, NTH15, decreases the expression of a gibberellin biosynthetic gene encoding GA-20 oxidase. Plant J 15:391–400PubMedCrossRefGoogle Scholar
  25. Tel-Zur N, Abbo S, Myslabodski D, Mizrahi Y (1999) Modified CTAB procedure for DNA isolation from epiphytic cacti of the genera Hylocereus and Selenicereus (Cactaceae). Plant Mol Biol Rep 17:249–254CrossRefGoogle Scholar
  26. Uchida N, Kimura S, Koenig D, Sinha N (2010) Coordination of leaf development via regulation of KNOX1 genes. J Plant Res 123:7–14PubMedCrossRefGoogle Scholar
  27. Wells R, Meredith WR Jr (1986) Normal vs. okra leaf yield interactions in cotton. II: analysis of vegetative and reproductive growth. Crop Sci 26:223–228CrossRefGoogle Scholar
  28. Wendel JF, Cronn RC (2003) Polyploidy and the evolutionary history of cotton. Adv Agron 78:139–186CrossRefGoogle Scholar
  29. Werner JE, Endo TR, Gill BS (1992) Toward a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci USA 89:11307–11311PubMedCrossRefGoogle Scholar
  30. Yan L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, SanMiguel P, Bennetzen JL, Echenique V, Dubcovsky J (2004) The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science 303:1640–1644PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ryan J. Andres
    • 1
  • Daryl T. Bowman
    • 1
  • Baljinder Kaur
    • 1
  • Vasu Kuraparthy
    • 1
    Email author
  1. 1.Crop Science DepartmentNorth Carolina State UniversityRaleighUSA

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