Mutations in chicory FEH genes are statistically associated with enhanced resistance to post-harvest inulin depolymerization
- 386 Downloads
Nucleotidic polymorphisms were identified in fructan exohydrolases genes which are statistically associated with enhanced susceptibility to post-harvest inulin depolymerization.
Industrial chicory (Cichorium intybus L.) root is the main commercial source of inulin, a linear fructose polymer used as dietary fiber. Post-harvest, inulin is depolymerized into fructose which drastically increases processing cost. To identify genetic variations associated with enhanced susceptibility to post-harvest inulin depolymerization and related free sugars content increase, we used a candidate-gene approach focused on inulin and sucrose synthesis and degradation genes, all members of the family 32 of glycoside hydrolases (GH32). Polymorphism in these genes was first investigated by carrying out EcoTILLING on two groups of chicory breeding lines exhibiting contrasted response to post-harvest inulin depolymerization. This allowed the identification of polymorphisms significantly associated with depolymerization in three fructan exohydrolase genes (FEH). This association was confirmed on a wider panel of 116 unrelated families in which the FEH polymorphism explained 35 % of the post-harvest variance for inulin content, 36 % of variance for sucrose content, 18 % for inulin degree of polymerization, 23 % for free fructose content and 22 % for free glucose content. These polymorphisms were associated with significant post-harvest changes of inulin content, inulin chain length and free sugars content.
KeywordsInulin Fructan Free Sugar Free Glucose Cell Wall Invertase
The authors acknowledge the Walloon Region (DGARNE-Belgium) for supporting this research (Grant D31-1221).
Conflict of interest
O. M. and C. N. are members of Cosucra-Group Warcoing S.A.
The authors acknowledge that the experiments described in this paper comply with the current laws of the country in which they were performed.
- CGPDB (2013) Lettuce Genome Resource. https://lgr.genomecenter.ucdavis.edu/Home.php. Accessed online 2013/05/20
- Closa-Monasterolo R, Gispert-Llaurado M, Luque V, Ferre N, Rubio-Torrents C, Zaragoza-Jordana M, Escribano J (2013) Safety and efficacy of inulin and oligofructose supplementation in infant formula: results from a randomized clinical trial. Clin NutrGoogle Scholar
- De Mendiburu F (2010) Agricolae: Statistical Procedures for Agricultural Research Using R (Online) http://tarwi.lamolina.edu.pe/~fmendiburu/. Accessed online 2013/03/05
- Di Bartolomeo F, Startek JB, Van den Ende W (2013) Prebiotics to fight diseases: reality or fiction? Phytother ResGoogle Scholar
- Maroufi A, Van Bockstaele E, De Loose M (2012) Differential expression of fructan 1-exohydrolase genes involved in inulin biodegradation in chicory (Cichorium intybus) cultivars. Aust J Crop Sci 6:1362–1368Google Scholar
- Muys C, Thienpont C-N, Dauchot N, Maudoux O, Draye X, Van Cutsem P (2013) Integration of AFLPs, SSRs and SNPs markers into a new genetic map of industrial chicory (Cichorium intybus L. va. sativum). Plant Breed. doi: 10.1111/pbr.12113
- R Development Core Team (2013) R: A language and environment for statistical coputing. R Foundation for Statistical Computing, ViennaGoogle Scholar
- SPW-Portail de l’Agriculture wallonne (2013) Catalogues nationaux des variétés d’espèces de plantes agricoles et des variétés d’espèces de légumes. http://agriculture.wallonie.be/apps/spip_wolwin/article.php3?id_article=68. Accessed online 2013/05/31
- van den Brink L, Wilting P (2012) Rassenbulletin cichorei. http://www.kennisakker.nl/kenniscentrum/document/rassenbulletin-cichorei. Accessed online 2013/05/31
- Van Waes C, Baert J, Carlier L, Van Bockstaele E (1998) A rapid determination of the total sugar content and the average inulin chain length in roots of chicory (Cichorium intybus L). J Sci Food Agric 76(1):107–110Google Scholar
- Vlaanderen-Landbouw en Visserij (2013) Industriële cichorei: resultaten van rassenproeven. http://lv.vlaanderen.be/nlapps/docs/default.asp?fid=153. Accessed online 2013/05/31