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Theoretical and Applied Genetics

, Volume 127, Issue 1, pp 51–57 | Cite as

Genetic analysis and molecular mapping of low amylose gene du12(t) in rice (Oryza sativa L.)

  • Gilang Kiswara
  • Jong-Hee Lee
  • Yeon-Jae Hur
  • Jun-Hyeon Cho
  • Ji-Yoon Lee
  • Sang-Yeol Kim
  • Yeong-Bo Sohn
  • You-Chun Song
  • Min-Hee Nam
  • Byung-Wook Yun
  • Kyung-Min KimEmail author
Original Paper

Abstract

Key message

We obtained interesting results for genetic analysis and molecular mapping of the du12(t) gene.

Abstract

Control of the amylose content in rice is the major strategy for breeding rice with improved quality. In this study, we conducted genetic analysis and molecular mapping to identify the dull gene in the dull rice, Milyang262. A single recessive gene, tentatively designated as du12(t), was identified as the dull gene that leads to the low amylose character of Milyang262. To investigate the inheritance of du12(t), genetic analysis on an F2 population derived from a cross between the gene carrier, Milyang262, and a moderate amylose content variety, Junam, was conducted. A segregation ratio of 3:1 (χ 2 = 1.71, p = 0.19) was observed, suggesting that du12(t) is a single recessive factor that controls the dull character in Milyang262. Allelism tests confirmed that du12(t) is not allelic to other low amylose controlling genes, wx or du1. Recessive class analysis was performed to localize the du12(t) locus. Mapping of du12(t) was conducted on F2 and F3 populations of Baegokchal/Milyang262 cross. Linkage analysis of 120 F2 plants revealed that RM6926 and RM3509 flank du12(t) at a 2.38-Mb region. To refine the du12(t) locus position, 986 F2 and 289 F3 additional normal plants were screened by the flanking markers. Twenty-six recombinant plants were identified and later genotyped with four additional adjacent markers located between RM6926 and RM3509. Finally, du12(t) was mapped to an 840-kb region on the distal region of the long arm of chromosome 6, delimited by SSR markers RM20662 and RM412, and co-segregated by RM3765 and RM176.

Keywords

Simple Sequence Repeat Marker Amylose Amylose Content Ethyl Methane Sulfonate Allelism Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

du

Dull

RM

Rice microsatellite

Notes

Acknowledgments

This work was supported by a grant from the Next-Generation Biogreen 21 Program (PJ0080912013) of the Rural Development Administration, Republic of Korea. We sincerely thank Kyungpook National University for their donation (International Scholarship Scheme) to G. Kiswara.

Conflict of interest

None.

Supplementary material

122_2013_2200_MOESM1_ESM.pdf (154 kb)
Supplementary material 1 (PDF 154 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Gilang Kiswara
    • 1
    • 2
  • Jong-Hee Lee
    • 1
  • Yeon-Jae Hur
    • 1
  • Jun-Hyeon Cho
    • 1
  • Ji-Yoon Lee
    • 1
  • Sang-Yeol Kim
    • 1
  • Yeong-Bo Sohn
    • 1
  • You-Chun Song
    • 1
  • Min-Hee Nam
    • 1
  • Byung-Wook Yun
    • 2
  • Kyung-Min Kim
    • 2
    Email author
  1. 1.Department of Functional Crop ScienceNICS, RDAMilyangKorea
  2. 2.Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life ScienceKyungpook National UniversityDaeguKorea

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