Inheritance of soybean aphid resistance in 21 soybean plant introductions
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The Rag2 region was frequently identified among 21 F 2 populations evaluated for soybean aphid resistance, and dominant gene action and single-gene resistance were also commonly identified.
The soybean aphid [Aphis glycines Matsumura (Hemiptera: Aphididae)] is one of the most important insect pests of soybean [Glycine max (L.) Merr] in the northern USA and southern Canada, and four resistance loci (Rag1–rag4) have been discovered since the pest was identified in the USA in 2000. The objective of this research was to determine whether resistance expression in recently identified soybean aphid-resistant plant introductions (PIs) was associated with the four Rag loci using a collection of 21 F2 populations. The F2 populations were phenotyped with soybean aphid biotype 1, which is avirulent on plants having any of the currently identified Rag genes, using choice tests in the greenhouse and were tested with genetic markers linked to the four Rag loci. The phenotyping results indicate that soybean aphid resistance is controlled by a single dominant gene in 14 PIs, by two genes in three PIs, and four PIs had no clear Mendelian inheritance patterns. Genetic markers flanking Rag2 were significantly associated with aphid resistance in 20 PIs, the Rag1 region was significantly identified in five PIs, and the Rag3 region was identified in one PI. These results show that single dominant gene action at the Rag2 region may be a major source for aphid resistance in the USDA soybean germplasm collection.
KeywordsSimple Sequence Repeat Marker Single Nucleotide Polymorphism Marker Plant Introduction Single Dominant Gene Aphid Resistance
Bulked segregant analysis
Polymerase chain reaction
Single nucleotide polymorphism
Simple sequence repeats
This work was supported by soybean check-off funding from the Illinois Soybean Association to B.D. and funding from the United Soybean Board to C.F. We also thank Laura Crull and Sarah Schultz for experiment assistance and support.
Conflict of interest
The authors declare that they have no conflict of interest.
The authors state that the experiments comply with the current laws of the country in which they were performed (USA).
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