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Theoretical and Applied Genetics

, Volume 127, Issue 1, pp 43–50 | Cite as

Inheritance of soybean aphid resistance in 21 soybean plant introductions

  • Carolyn M. FoxEmail author
  • Ki-Seung Kim
  • Perry B. Cregan
  • Curtis B. Hill
  • Glen L. Hartman
  • Brian W. Diers
Original Paper

Abstract

Key Message

The Rag2 region was frequently identified among 21 F 2 populations evaluated for soybean aphid resistance, and dominant gene action and single-gene resistance were also commonly identified.

Abstract

The soybean aphid [Aphis glycines Matsumura (Hemiptera: Aphididae)] is one of the most important insect pests of soybean [Glycine max (L.) Merr] in the northern USA and southern Canada, and four resistance loci (Rag1rag4) have been discovered since the pest was identified in the USA in 2000. The objective of this research was to determine whether resistance expression in recently identified soybean aphid-resistant plant introductions (PIs) was associated with the four Rag loci using a collection of 21 F2 populations. The F2 populations were phenotyped with soybean aphid biotype 1, which is avirulent on plants having any of the currently identified Rag genes, using choice tests in the greenhouse and were tested with genetic markers linked to the four Rag loci. The phenotyping results indicate that soybean aphid resistance is controlled by a single dominant gene in 14 PIs, by two genes in three PIs, and four PIs had no clear Mendelian inheritance patterns. Genetic markers flanking Rag2 were significantly associated with aphid resistance in 20 PIs, the Rag1 region was significantly identified in five PIs, and the Rag3 region was identified in one PI. These results show that single dominant gene action at the Rag2 region may be a major source for aphid resistance in the USDA soybean germplasm collection.

Keywords

Simple Sequence Repeat Marker Single Nucleotide Polymorphism Marker Plant Introduction Single Dominant Gene Aphid Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BSA

Bulked segregant analysis

MG

Maturity group

PCR

Polymerase chain reaction

PI

Plant introduction

SNP

Single nucleotide polymorphism

SSR

Simple sequence repeats

Notes

Acknowledgments

This work was supported by soybean check-off funding from the Illinois Soybean Association to B.D. and funding from the United Soybean Board to C.F. We also thank Laura Crull and Sarah Schultz for experiment assistance and support.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The authors state that the experiments comply with the current laws of the country in which they were performed (USA).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Carolyn M. Fox
    • 1
    Email author
  • Ki-Seung Kim
    • 1
    • 4
  • Perry B. Cregan
    • 3
  • Curtis B. Hill
    • 1
  • Glen L. Hartman
    • 1
    • 2
  • Brian W. Diers
    • 1
  1. 1.Department of Crop SciencesUniversity of IllinoisUrbanaUSA
  2. 2.USDA-ARSUrbanaUSA
  3. 3.Soybean Genomics and Improvement LaboratoryUSDA-ARSBeltsvilleUSA
  4. 4.The Samuel Roberts Noble FoundationArdmoreUSA

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