Theoretical and Applied Genetics

, Volume 126, Issue 10, pp 2477–2484

Introgression of stem rust resistance genes SrTA10187 and SrTA10171 from Aegilops tauschii to wheat

  • Eric L. Olson
  • Matthew N. Rouse
  • Michael O. Pumphrey
  • Robert L. Bowden
  • Bikram S. Gill
  • Jesse A. Poland
Original Paper

Abstract

Aegilops tauschii, the diploid progenitor of the wheat D genome, is a readily accessible germplasm pool for wheat breeding as genes can be transferred to elite wheat cultivars through direct hybridization followed by backcrossing. Gene transfer and genetic mapping can be integrated by developing mapping populations during backcrossing. Using direct crossing, two genes for resistance to the African stem rust fungus race TTKSK (Ug99), were transferred from the Ae. tauschii accessions TA10187 and TA10171 to an elite hard winter wheat line, KS05HW14. BC2 mapping populations were created concurrently with developing advanced backcross lines carrying rust resistance. Bulked segregant analysis on the BC2 populations identified marker loci on 6DS and 7DS linked to stem rust resistance genes transferred from TA10187 and TA10171, respectively. Linkage maps were developed for both genes and closely linked markers reported in this study will be useful for selection and pyramiding with other Ug99-effective stem rust resistance genes. The Ae. tauschii-derived resistance genes were temporarily designated SrTA10187 and SrTA10171 and will serve as valuable resources for stem rust resistance breeding.

References

  1. Anugrahwati DR, Shepherd KW, Verlin DC, Zhang P, Mirzaghaderi G, Walker E, Francki MG, Dundas IS (2008) Isolation of wheat-rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin. Genome 51:341–349PubMedCrossRefGoogle Scholar
  2. Cox TS (1998) Deepening the wheat gene pool. J Crop Prod 1:1–25CrossRefGoogle Scholar
  3. Cox TS, Sears RG, Bequette RK (1995a) Use of winter wheat × Triticum tauschii backcross populations for germplasm evaluation. Theor Appl Genet 90:571–577CrossRefGoogle Scholar
  4. Cox TS, Sears RG, Bequette RK, Martin TJ (1995b) Germplasm enhancement in winter wheat × Triticum tauschii backcross populations. Crop Sci 35:913–919CrossRefGoogle Scholar
  5. Dundas IS, Anugrahwati DR, Verlin DC, Park RF, Bariana HS, Mago R, Islam AKMR (2007) New sources of rust resistance from alien species: meliorating linked defects and discovery. Aust J Agric Res 58:545–549CrossRefGoogle Scholar
  6. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158PubMedCrossRefGoogle Scholar
  7. Ghazvini H, Hiebert CW, Zegeye T, Liu S, Dilawari M, Tsilo T, Anderson JA, Rouse MN, Jin Y, Fetch T (2012) Inheritance of resistance to Ug99 stem rust in wheat cultivar Norin 40 and genetic mapping of Sr42. Theor Appl Genet 4:817–824CrossRefGoogle Scholar
  8. Gill BS, Raupp WJ (1987) Direct genetic transfers from Aegilops squarrosa L. to hexaploid wheat. Crop Sci 27:445–450CrossRefGoogle Scholar
  9. Hiebert CW, Fetch TG, Zegeye T, Thomas JB, Somers DJ, Humphreys DG, McCallum BD, Cloutier S, Singh D, Knott DR (2011) Genetics and mapping of seedling resistance to Ug99 stem rust in Canadian wheat cultivars ‘Peace’ and ‘AC Cadillac’. Theor Appl Genet 122:143–149PubMedCrossRefGoogle Scholar
  10. Jin Y, Singh RP, Ward RW, Wanyera R, Kinyua M, Njau P, Fetch T, Pretorius ZA, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 91:1096–1099CrossRefGoogle Scholar
  11. Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward R, Fetch T Jr (2008) Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 92:923–926CrossRefGoogle Scholar
  12. Kerber ER, Dyck PL (1979) Resistance to stem and leaf rust of wheat in Aegilops squarrosa and transfer of a gene for stem rust resistance to hexaploid wheat. In: Ramanjuam S (ed) Proceedings of the 5th international wheat genetics symposium. New Delhi, India, pp 358–364Google Scholar
  13. Klindworth DL, Niu Z, Chao S, Friesen TL, Jin Y, Faris JD, Cai X, Xu SS (2012) Introgression and characterization of a goatgrass gene for a high level of resistance to Ug99 stem rust in tetraploid wheat. G3 Bethesda 2:665–673PubMedCrossRefGoogle Scholar
  14. Kolmer J, Garvin DF, Jin Y (2011) Expression of a Thatcher wheat adult plant stem rust resistance QTL on chromosome arm 2BL is enhanced by Lr34. Crop Sci 51:526–533CrossRefGoogle Scholar
  15. Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363PubMedCrossRefGoogle Scholar
  16. Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Wolfgang Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898PubMedCrossRefGoogle Scholar
  17. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental an natural populations. Genomics 2:174–181CrossRefGoogle Scholar
  18. Liu W, Jin Y, Rouse M, Friebe B, Gill B, Pumphrey MO (2011a) Development and characterization of wheat-Ae. searsii Robertsonian translocations and a recombinant chromosome conferring resistance to stem rust. Theor Appl Genet 122:1537–1545PubMedCrossRefGoogle Scholar
  19. Liu W, Rouse M, Friebe B, Jin Y, Gill B, Pumphrey MO (2011b) Discovery and molecular mapping of a new gene conferring resistance to stem rust, Sr53, derived from Aegilops geniculata and characterization of spontaneous translocation stocks with reduced alien chromatin. Chromosome Res 19:669–682PubMedCrossRefGoogle Scholar
  20. Liu W, Danilova TV, Rouse M, Bowden RL, Friebe B, Gill BS, Pumphrey MO (2013) Development and characterization of a compensating wheat-Thinopyrum intermedium Robertsonian translocation with Sr44 resistance to stem rust (Ug99). Theor Appl Genet. doi:10.1007/s00122-013-2044-6 Google Scholar
  21. McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts, an atlas of resistance genes. CSIRO, East MelbourneCrossRefGoogle Scholar
  22. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  23. Niu Z, Klindworth DL, Friesen TL, Chao S, Jin Y, Cai X, Xu SS (2011) Targeted introgression of a wheat stem rust resistance gene by DNA marker-assisted chromosome engineering. Genetics 187:1011–1021PubMedCrossRefGoogle Scholar
  24. Olson EL, Brown-Guedira G, Marshall DS, Jin Y, Mergoum M, Lowe I, Dubcovsky J (2010a) Genotyping of US wheat germplasm for presence of stem rust resistance genes Sr24, Sr36 and Sr1RSAmigo. Crop Sci 50:668–675CrossRefGoogle Scholar
  25. Olson EL, Brown-Guedira G, Marshall D, Stack E, Bowden RL, Jin Y, Rouse MN, Pumphrey MO (2010b) Development of wheat lines having a small introgressed segment carrying stem rust resistance gene Sr22. Crop Sci 50:1823–1830CrossRefGoogle Scholar
  26. Olson EL, Rouse M, Pumphrey MO, Bowden R, Gill BS, Poland JA (2013) Simultaneous transfer, introgression, and genomic localization of genes for resistance to stem rust race TTKSK (Ug99) from Aegilops tauschii to wheat. Theor Appl Genet. doi:10.1007/s00122-013-2045-5 Google Scholar
  27. Pretorius Z, Singh R, Wagoire W, Payne T (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis. f. sp. tritici in Uganda. Plant Dis 84:203CrossRefGoogle Scholar
  28. Qi LL, Pumphrey MO, Friebe B, Zhang P, Qian C, Bowden RL, Rouse MN, Jin Y, Gill BS (2011) A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet 123:159–167PubMedCrossRefGoogle Scholar
  29. Roelfs AP, Martens JW (1987) An international system of nomenclature for Puccinia graminis f. sp. tritici. Phytopathology 78:526–533CrossRefGoogle Scholar
  30. Rosenquist CE (1927) An improved method of producing F1 hybrid seeds of wheat and barley. Agron J 19:968–971CrossRefGoogle Scholar
  31. Rouse MN, Olson EL, Gill BS, Pumphrey MO, Jin Y (2011) Stem rust resistance in Aegilops tauschii germplasm. Crop Sci 51:2074–2078CrossRefGoogle Scholar
  32. Rubiales D, Niks RE (1995) Characterisation of Lr34, a major gene conferring non-hypersensitive resistance to wheat leaf rust. Plant Dis 79:1208–1212CrossRefGoogle Scholar
  33. Sears ER (1948) The cytology and genetics of the wheats and their relatives. Adv Genet 2:239–270CrossRefGoogle Scholar
  34. Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Bhavani S, Njau P, Herrera-Foessel S, Singh PK, Singh S, Govindan V (2011) The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annu Rev Phytopathol 49:465–481PubMedCrossRefGoogle Scholar
  35. Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111:731–735PubMedCrossRefGoogle Scholar
  36. Stakman E, Steward D, Loegering W (1962) Identification of physiologic races of Puccinia graminis var. tritici. US Department of Agriculture, ARS, Washington, p E-617Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2013

Authors and Affiliations

  • Eric L. Olson
    • 1
    • 6
  • Matthew N. Rouse
    • 2
  • Michael O. Pumphrey
    • 3
  • Robert L. Bowden
    • 1
    • 4
  • Bikram S. Gill
    • 1
  • Jesse A. Poland
    • 4
    • 5
  1. 1.Department of Plant PathologyKansas State UniversityManhattanUSA
  2. 2.USDA-ARS Cereal Disease LaboratoryUniversity of MinnesotaSt. PaulUSA
  3. 3.Department of Crop and Soil SciencesWashington State UniversityPullmanUSA
  4. 4.Hard Winter Wheat Genetics Research UnitUSDA-ARSManhattanUSA
  5. 5.Department of AgronomyKansas State UniversityManhattanUSA
  6. 6.Department of Plant and Soil ScienceMichigan State UniversityEast LansingUSA

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