Theoretical and Applied Genetics

, Volume 126, Issue 10, pp 2477–2484

Introgression of stem rust resistance genes SrTA10187 and SrTA10171 from Aegilops tauschii to wheat

  • Eric L. Olson
  • Matthew N. Rouse
  • Michael O. Pumphrey
  • Robert L. Bowden
  • Bikram S. Gill
  • Jesse A. Poland
Original Paper

DOI: 10.1007/s00122-013-2148-z

Cite this article as:
Olson, E.L., Rouse, M.N., Pumphrey, M.O. et al. Theor Appl Genet (2013) 126: 2477. doi:10.1007/s00122-013-2148-z

Abstract

Aegilops tauschii, the diploid progenitor of the wheat D genome, is a readily accessible germplasm pool for wheat breeding as genes can be transferred to elite wheat cultivars through direct hybridization followed by backcrossing. Gene transfer and genetic mapping can be integrated by developing mapping populations during backcrossing. Using direct crossing, two genes for resistance to the African stem rust fungus race TTKSK (Ug99), were transferred from the Ae. tauschii accessions TA10187 and TA10171 to an elite hard winter wheat line, KS05HW14. BC2 mapping populations were created concurrently with developing advanced backcross lines carrying rust resistance. Bulked segregant analysis on the BC2 populations identified marker loci on 6DS and 7DS linked to stem rust resistance genes transferred from TA10187 and TA10171, respectively. Linkage maps were developed for both genes and closely linked markers reported in this study will be useful for selection and pyramiding with other Ug99-effective stem rust resistance genes. The Ae. tauschii-derived resistance genes were temporarily designated SrTA10187 and SrTA10171 and will serve as valuable resources for stem rust resistance breeding.

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2013

Authors and Affiliations

  • Eric L. Olson
    • 1
    • 6
  • Matthew N. Rouse
    • 2
  • Michael O. Pumphrey
    • 3
  • Robert L. Bowden
    • 1
    • 4
  • Bikram S. Gill
    • 1
  • Jesse A. Poland
    • 4
    • 5
  1. 1.Department of Plant PathologyKansas State UniversityManhattanUSA
  2. 2.USDA-ARS Cereal Disease LaboratoryUniversity of MinnesotaSt. PaulUSA
  3. 3.Department of Crop and Soil SciencesWashington State UniversityPullmanUSA
  4. 4.Hard Winter Wheat Genetics Research UnitUSDA-ARSManhattanUSA
  5. 5.Department of AgronomyKansas State UniversityManhattanUSA
  6. 6.Department of Plant and Soil ScienceMichigan State UniversityEast LansingUSA

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