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Characterization and fine mapping of the rice premature senescence mutant ospse1

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Abstract

Premature senescence can limit crop productivity by limiting the growth phase. In the present study, a spontaneous premature senescence mutant was identified in rice (Oryza sativa L.). Genetic analysis revealed that the premature senescence phenotype was controlled by a recessive mutation, which we named Oryza sativa premature senescence1 (ospse1). The ospse1 mutants showed premature leaf senescence from the booting stage and exhibited more severe symptoms during reproductive and ripening stages. Key yield-related agronomic traits such as 1,000-grain weight and seed-setting rate, but not panicle grain number, were significantly reduced in ospse1 plants. Chlorophyll content, net photosynthetic rate, and transpiration rate of ospse1 flag leaves were similar to the wild-type plants in vegetative stages, but these parameters decreased steeply in the mutant after the heading stage. Consistent with this, the senescence-associated genes OsNYC1 and OsSgr were up-regulated in ospse1 mutant during premature leaf senescence. The ospse1 locus was mapped to a 38-kb region on chromosome 1 and sequence analysis of this region identified a single-nucleotide deletion in the 3′ region of an open reading frame (ORF) encoding a putative pectate lyase, leading to a frame shift and a longer ORF. Our results suggested that the premature senescence of the ospse1 may be regulated by a novel mechanism mediated by pectate lyase.

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Abbreviations

ABA:

Abscisic acid

CC:

Chlorophyll content

MeJA:

Methyl jasmonate acid

NPR:

Net photosynthetic rate

ORF:

Open reading frame

SAG:

Senescence-associated gene

SPAD:

Soil plant analysis development

TR:

Transpiration rate

qRT-PCR:

Quantitative reverse transcription polymerase chain reaction

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Acknowledgments

This work was supported by a grant from the National High Technology Research and Development Program of China (2012AA10A303); and a grant from the Talent Introduction Projects of Guangdong Provincial Colleges and Universities (2011) to L. Chen.

Conflict of interest

The authors declare that they have no conflicts of interests.

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China

    Hai-Bin Wu, Bin Wang, Yuanling Chen, Yao-Guang Liu & Letian Chen

  2. Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China

    Letian Chen

  3. Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Hai-Bin Wu

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  1. Hai-Bin Wu
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  2. Bin Wang
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  3. Yuanling Chen
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  4. Yao-Guang Liu
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  5. Letian Chen
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Correspondence to Yao-Guang Liu or Letian Chen.

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Communicated by T. Sasaki.

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Wu, HB., Wang, B., Chen, Y. et al. Characterization and fine mapping of the rice premature senescence mutant ospse1 . Theor Appl Genet 126, 1897–1907 (2013). https://doi.org/10.1007/s00122-013-2104-y

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