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Theoretical and Applied Genetics

, Volume 126, Issue 7, pp 1783–1791 | Cite as

Genetic analysis and mapping of genes for resistance to multiple strains of Soybean mosaic virus in a single resistant soybean accession PI 96983

  • Yongqing Yang
  • Guijie Zheng
  • Lu Han
  • Wang Dagang
  • Xiaofeng Yang
  • Yuan Yuan
  • Saihua Huang
  • Haijian ZhiEmail author
Original Paper

Abstract

Soybean mosaic virus (SMV) is one of the most broadly distributed soybean (Glycine max (L.) Merr.) diseases and causes severe yield loss and seed quality deficiency. Multiple studies have proved that a single dominant gene can confer resistance to several SMV strains. Plant introduction (PI) 96983 has been reported to contain SMV resistance genes (e.g., Rsv1 and Rsc14) on chromosome 13. The objective of this study was to delineate the genetics of resistance to SMV in PI 96983 and determine whether one gene can control resistance to more than one Chinese SMV strain. In this study, PI 96983 was identified as resistant and Nannong 1138-2 was identified as susceptible to four SMV strains SC3, SC6, SC7, and SC17. Genetic maps based on 783 F2 individuals from the cross of PI 96983 × Nannong 1138-2 showed that the gene(s) conferring resistance to SC3, SC6, and SC17 were between SSR markers BARCSOYSSR_13_1114 and BARCSOYSSR_13_1136, whereas SC7 was between markers BARCSOYSSR_13_1140 and BARCSOYSSR_13_1185. The physical map based on 58 recombinant lines confirmed these results. The resistance gene for SC7 was positioned between BARCSOYSSR_13_1140 and BARCSOYSSR_13_1155, while the resistance gene(s) for SC3, SC6, and SC17 were between BARCSOYSSR_13_1128 and BARCSOYSSR_13_1136. We concluded that, there were two dominant resistance genes flanking Rsv1 or one of them at the reported genomic location of Rsv1. One of them (designated as “Rsc-pm”) conditions resistance for SC3, SC6, and SC17 and another (designated as “Rsc-ps”) confers resistance for SC7. The two tightly linked genes identified in this study would be helpful to cloning of resistance genes and breeding of multiple resistances soybean cultivars to SMV through marker-assisted selection (MAS).

Keywords

Simple Sequence Repeat Marker Plant Introduction Recombinant Line Soybean Mosaic Virus Pymetrozine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (GrantNo.30971815, 31171574), the National Soybean Industrial Technology System of China (No. CARS-004) and the Fund of Transgenic Breeding for Soybean Resistance to Soybean Mosaic Virus (No.2008ZX08004-004). Our sincere thanks also extended to Mr. Song Q.J. from Soybean Genomics and Improvement Laboratory, USDA-ARS for his providing the SSR markers primer sequences.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Yongqing Yang
    • 1
  • Guijie Zheng
    • 1
  • Lu Han
    • 2
  • Wang Dagang
    • 1
    • 4
  • Xiaofeng Yang
    • 1
    • 3
  • Yuan Yuan
    • 1
  • Saihua Huang
    • 1
  • Haijian Zhi
    • 1
    Email author
  1. 1.National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina
  2. 2.The Zhongbei College of Nanjing Normal UniversityNanjingChina
  3. 3.Hunan Crop Research InstituteChangshaChina
  4. 4.Anhui Key Lab of Crops Quality Improving/Crop Institute of Anhui Academy of Agricultural SciencesHefeiChina

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